Abstract
Aim. To investigate the resistance to water deficit of transgenic plants of new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene under physiological and stress conditions. Methods. Genetic transformation by the in planta method, physiological and biochemical, mathematical statistics. Results. In transgenic T3 plants, it was confirmed that the increased activity of the ornithine-δ-aminotransferase enzyme does not significantly affect the content of free proline either under control conditions or under water deficit conditions. It was confirmed that overexpression of the oat gene promotes more active root development in genetically modified plants both under normal and drought conditions: in terms of root length, they exceeded the plants of the original genotypes by 3.9–4.5 cm under normal irrigation, and by 3.3–3.8 cm under drought conditions. Under soil drought conditions, biotechnological plants outperformed the original ones by 14–19 % in terms of grain quantity per plant, and by 10–16 % in terms of grain mass per plant. Conclusions. It was shown that transgenic T3 plants with overexpression of the oat gene are characterized by relatively higher resistance to soil drought conditions than control plants. Activation of growth processes, which is manifested in an increase in stem height and better development of the root system, improves adaptive plasticity and yield of genetically modified plants.
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Claims 8
Claim · model-written summary
Increased ornithine-δ-aminotransferase activity did not significantly affect free proline content under control or water deficit conditions.
- Taxon
- winter soft wheate0
- Treatment
- overexpression of the proline synthesis genee0
- Gene
- ornithine-δ-aminotransferasee1
- Outcome
- content of free prolinee1
- Direction
- no_changeinferrede1
Not reportedComparator
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e1“In transgenic T3 plants, it was confirmed that the increased activity of the ornithine-δ-aminotransferase enzyme does not significantly affect the content of free proline either under control conditions or under water deficit conditions”
Claim · model-written summary
Overexpression of the oat gene increased root length by 3.9–4.5 cm relative to original genotypes under normal irrigation.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e2“overexpression of the oat gene promotes more active root development in genetically modified plants both under normal and drought conditions: in terms of root length, they exceeded the plants of the original genotypes by 3.9–4.5 cm under normal irrigation, and by 3.3–3.8 cm under drought conditions”
Claim · model-written summary
Overexpression of the oat gene increased root length by 3.3–3.8 cm relative to original genotypes under drought conditions.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e2“overexpression of the oat gene promotes more active root development in genetically modified plants both under normal and drought conditions: in terms of root length, they exceeded the plants of the original genotypes by 3.9–4.5 cm under normal irrigation, and by 3.3–3.8 cm under drought conditions”
Claim · model-written summary
Under soil drought, transgenic plants outperformed the original ones by 14–19% in grain quantity per plant.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e3“Under soil drought conditions, biotechnological plants outperformed the original ones by 14–19 % in terms of grain quantity per plant, and by 10–16 % in terms of grain mass per plant”
Claim · model-written summary
Under soil drought, transgenic plants outperformed the original ones by 10–16% in grain mass per plant.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e3“Under soil drought conditions, biotechnological plants outperformed the original ones by 14–19 % in terms of grain quantity per plant, and by 10–16 % in terms of grain mass per plant”
Claim · model-written summary
Transgenic plants with oat gene overexpression had relatively higher resistance to soil drought than control plants.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e4“transgenic T3 plants with overexpression of the oat gene are characterized by relatively higher resistance to soil drought conditions than control plants”
Claim · model-written summary
Activation of growth processes improves adaptive plasticity and yield of genetically modified wheat.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e5“Activation of growth processes, which is manifested in an increase in stem height and better development of the root system, improves adaptive plasticity and yield of genetically modified plants”
Claim · model-written summary
Genetically modified wheat showed an increase in stem height.
Verbatim
e0“new genotypes of winter soft wheat of the T3 seed generation with overexpression of the proline synthesis gene”
e5“Activation of growth processes, which is manifested in an increase in stem height and better development of the root system, improves adaptive plasticity and yield of genetically modified plants”
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proline · physiology: osmolytes · Triticum aestivum (wheat)Positive and negative results
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.