Abstract
Excessive nitrogen (N) fertilization exacerbates rice blast disease caused by Magnaporthe oryzae, yet the underlying microbiological mechanisms remain unclear. Through integrated field surveys and controlled experiments, we demonstrate that high-N application worsens disease severity by disrupting microbiome communities in the phyllosphere of rice (Oryza sativa) plants. We identified microorganisms in the phyllosphere through 16S rDNA sequencing. Low-N conditions were associated with a greater Massilia bacterial population, which was negatively correlated with pathogen abundance and disease index. In vitro and in vivo experiments indicated that Massilia enhances rice resistance to rice blast disease. We explored how Massilia confers resistance. Massilia strain z571 directly inhibited M. oryzae spore germination and hyphal invasion while also activating host immunity, including elevated levels of salicylic acid and jasmonic acid, enhanced expression of defense-related genes, and increased activity of antioxidant defense enzymes. Moreover, Massilia exhibited broad-spectrum antagonism against multiple pathogens. Our findings unveil a microbiome-mediated mechanism linking N fertilization to disease susceptibility and highlight Massilia as a potential biocontrol agent, offering a sustainable strategy for managing rice blast through optimized fertilization practices and microbial interventions.
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Claims 13
Claim · model-written summary
High-N application worsened rice blast disease severity.
- Taxon
- rice
Oryza sativae0 - Treatment
- high-N applicatione0
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaee1pathogen - Outcome
- disease severitye0
- Direction
- worsense0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-N application worsens disease severity by disrupting microbiome communities in the phyllosphere of rice (Oryza sativa) plants”
e1“rice blast disease caused by Magnaporthe oryzae”
Claim · model-written summary
High-N application disrupted rice phyllosphere microbiome communities.
- Taxon
- riceOryza sativae0
- Treatment
- high-N applicatione0
- Outcome
- microbiome communities in the phyllospheree0
- Direction
- disruptinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-N application worsens disease severity by disrupting microbiome communities in the phyllosphere of rice (Oryza sativa) plants”
Claim · model-written summary
Low-N conditions were associated with a greater Massilia bacterial population in rice phyllosphere.
- Taxon
- riceOryza sativae1
- Treatment
- Low-N conditionse0
- Outcome
- Massilia bacterial populatione0
- Direction
- greatere0
MassiliaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-N conditions were associated with a greater Massilia bacterial population”
e1“phyllosphere of rice (Oryza sativa) plants”
Claim · model-written summary
Massilia population was negatively correlated with pathogen abundance.
- Taxon
- riceOryza sativae1
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Outcome
- pathogen abundancee0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low-N conditions were associated with a greater Massilia bacterial population, which was negatively correlated with pathogen abundance and disease index.”
e1“phyllosphere of rice (Oryza sativa) plants”
e2“Magnaporthe oryzae”
Claim · model-written summary
Massilia population was negatively correlated with rice blast disease index.
- Taxon
- riceOryza sativae1
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Outcome
- disease indexe0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia bacterial population, which was negatively correlated with pathogen abundance and disease index”
e1“phyllosphere of rice (Oryza sativa) plants”
e2“Magnaporthe oryzae”
Claim · model-written summary
Massilia enhanced rice resistance to rice blast disease.
- Taxon
- riceOryza sativae0
- Treatment
- Massiliae0
- Outcome
- rice resistance to rice blast diseasee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia enhances rice resistance to rice blast disease”
Claim · model-written summary
Massilia strain z571 directly inhibited M. oryzae spore germination.
- Taxon
- riceOryza sativae1
- Treatment
- Massilia strain z571e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Second interactor
- Massilia strain z571e0biocontrol_agent
- Outcome
- M. oryzae spore germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia strain z571 directly inhibited M. oryzae spore germination and hyphal invasion”
e1“rice (Oryza sativa) plants”
Claim · model-written summary
Massilia strain z571 directly inhibited M. oryzae hyphal invasion.
- Taxon
- riceOryza sativae1
- Treatment
- Massilia strain z571e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Second interactor
- Massilia strain z571e0biocontrol_agent
- Outcome
- hyphal invasione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia strain z571 directly inhibited M. oryzae spore germination and hyphal invasion”
e1“rice (Oryza sativa) plants”
Claim · model-written summary
Massilia strain z571 elevated rice host salicylic acid and jasmonic acid levels.
- Taxon
- riceOryza sativae1
- Treatment
- Massilia strain z571e0
- Interactor
- Massilia strain z571e0biocontrol_agent
- Outcome
- levels of salicylic acid and jasmonic acide0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia strain z571 directly inhibited M. oryzae spore germination and hyphal invasion while also activating host immunity, including elevated levels of salicylic acid and jasmonic acid”
e1“rice (Oryza sativa) plants”
Claim · model-written summary
Massilia strain z571 enhanced host defense-related gene expression.
- Taxon
- riceOryza sativae2
- Treatment
- Massilia strain z571e0
- Interactor
- Massilia strain z571e0biocontrol_agent
- Outcome
- expression of defense-related genese1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia strain z571”
e1“enhanced expression of defense-related genes”
e2“rice (Oryza sativa) plants”
Claim · model-written summary
Massilia strain z571 increased antioxidant defense enzyme activity in the host.
- Taxon
- riceOryza sativae2
- Treatment
- Massilia strain z571e0
- Interactor
- Massilia strain z571e0biocontrol_agent
- Outcome
- activity of antioxidant defense enzymese1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia strain z571”
e1“increased activity of antioxidant defense enzymes”
e2“rice (Oryza sativa) plants”
Claim · model-written summary
Massilia showed broad-spectrum antagonism against multiple pathogens.
- Taxon
- riceOryza sativae1
- Outcome
- broad-spectrum antagonism against multiple pathogense0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Massilia exhibited broad-spectrum antagonism against multiple pathogens”
e1“rice (Oryza sativa) plants”
Claim · model-written summary
The authors present optimized fertilization and microbial intervention as a sustainable rice blast management strategy.
- Taxon
- riceOryza sativae1
- Outcome
- managing rice blast through optimized fertilization practices and microbial interventionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“offering a sustainable strategy for managing rice blast through optimized fertilization practices and microbial interventions”
e1“rice (Oryza sativa) plants”
The same questions in other papers
nitrogen · Pyricularia oryzae · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
nitrogen · soil: biology · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 19 in the opposite direction
- positive
- positive
- positive
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.