Abstract
High temperature stress poses a significant threat to the normal growth of maize seedlings, and key heat-resistant gene mining is the molecular basis for breeding new heat-resistant maize varieties. Through transcriptome sequencing of heat-tolerant hybrid ZD819 and its parental lines (ZD819-F, ZD819-M) under high-temperature stress, we identified 12 HSF (Heat shock transcription factors, HSFs) transcription factors from 12,442 differentially expressed genes. The results indicate that the maize hybrid ZD819 has stronger heat tolerance compared to its parent varieties (ZD819-F, ZD819-M). Transcriptome data identified 12 HSFs transcription factors, among which ZmHSF10 had the highest differential expression fold of 1279.40 before and after high-temperature treatment. The heat tolerance function of ZmHSF10 was studied by creating Arabidopsis thaliana materials overexpressing ZmHSF10 and obtaining ZmHSF10 silenced maize materials using VIGS technology. Genetic experiments have shown that overexpression of ZmHSF10 can stabilize the cell membrane stability of Arabidopsis plants under high temperature stress and improve their survival rate under high temperature treatment. Reducing the expression level of ZmHSF10 leads to a decrease in chlorophyll content, poor cell membrane stability, and lower relative water content in maize leaves under high temperature stress. These results preliminarily demonstrate that ZmHSF10 plays an important role in regulating heat tolerance in plants, providing genetic resources for enhancing heat tolerance in maize seedlings.
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Claims 6
Claim · model-written summary
Maize hybrid ZD819 showed stronger heat tolerance than its parent varieties ZD819-F and ZD819-M.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maize hybrid ZD819 has stronger heat tolerance compared to its parent varieties (ZD819-F, ZD819-M)”
Claim · model-written summary
Transcriptome sequencing under high-temperature stress identified 12 HSF transcription factors among 12,442 differentially expressed genes in maize.
- Outcome
- 12 HSF (Heat shock transcription factors, HSFs) transcription factorse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“we identified 12 HSF (Heat shock transcription factors, HSFs) transcription factors from 12,442 differentially expressed genes”
Claim · model-written summary
Among the 12 HSFs, ZmHSF10 had the highest differential expression fold, 1279.40, before versus after high-temperature treatment.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmHSF10 had the highest differential expression fold of 1279.40 before and after high-temperature treatment”
Claim · model-written summary
Overexpression of ZmHSF10 in Arabidopsis stabilized cell membrane stability and improved survival rate under high temperature stress.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of ZmHSF10e0
- Gene
- ZmHSF10e0
- Outcome
- cell membrane stabilitye0
- Direction
- stabilizee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of ZmHSF10 can stabilize the cell membrane stability of Arabidopsis plants under high temperature stress and improve their survival rate under high temperature treatment”
Claim · model-written summary
Reducing ZmHSF10 expression in maize (VIGS-silenced) decreased chlorophyll content, cell membrane stability and relative water content in leaves under high temperature stress.
- Organ / stage
- leavese0
- Treatment
- Reducing the expression level of ZmHSF10e0
- Gene
- ZmHSF10e0
- Outcome
- chlorophyll content, poor cell membrane stability, and lower relative water contente0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reducing the expression level of ZmHSF10 leads to a decrease in chlorophyll content, poor cell membrane stability, and lower relative water content in maize leaves under high temperature stress”
Claim · model-written summary
The authors conclude preliminarily that ZmHSF10 plays an important role in regulating heat tolerance in plants.
- Taxon
- plantsViridiplantaee0
- Gene
- ZmHSF10e0
- Outcome
- heat tolerancee0
Verbatim
e0“ZmHSF10 plays an important role in regulating heat tolerance in plants”