Abstract
SUMMARY Salinity represents a major abiotic stressor that significantly impairs soybean growth and yield. Although jasmonic acid (JA) has been firmly established as a key regulator of plant defense against salt stress, the precise functions of lipoxygenase ( LOX ) genes responsible for initiating JA biosynthesis remain poorly defined. Here, a comprehensive genome‐wide analysis of the soybean LOX gene family was performed, and a detailed functional characterization of GmLOX6 was carried out. Subcellular localization confirmed that GmLOX6 is targeted to chloroplasts, while enzymatic assays demonstrated that it acts as a 13‐LOX enzyme with a strong preference for α‐linolenic acid as substrate. To clarify its role under salt stress, we generated both overexpression and CRISPR/Cas9‐mediated knockout lines of soybean. Phenotypic and molecular evaluations revealed that GmLOX6 facilitates JA production under salt stress, thereby contributing to enhanced JA accumulation. This elevation in JA levels was associated with improved salt tolerance through multiple physiological adaptations, including the activation of antioxidant enzymes for the detoxification of reactive oxygen species (ROS), enhanced Na + extrusion to preserve ionic balance, and reinforced membrane stability. Moreover, GmRWP‐RK11 was identified as a transcriptional repressor of GmLOX6 . Functional disruption of GmRWP‐RK11 via CRISPR/Cas9 conferred greater salt tolerance, further supporting its negative regulatory role. Collectively, these findings uncover a novel regulatory axis in which GmLOX6 ‐mediated JA biosynthesis enhances soybean resistance to salinity through modulation of ROS homeostasis and Na + transport. These insights provide an expanded understanding of the transcriptional and biochemical mechanisms underpinning JA‐driven stress adaptation in soybean.
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Claims 9
Claim · model-written summary
GmLOX6 acts as a 13-LOX enzyme with a strong preference for alpha-linolenic acid.
- Taxon
- soybean
Glycine maxt0 - Gene
- GmLOX6e1
- Compound
- α‐linolenic acide2
- Outcome
- acts as a 13‐LOX enzymee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e1“Subcellular localization confirmed that GmLOX6 is targeted to chloroplasts”
e2“enzymatic assays demonstrated that it acts as a 13‐LOX enzyme with a strong preference for α‐linolenic acid as substrate”
Claim · model-written summary
GmLOX6 is localized to chloroplasts.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmLOX6e1
- Outcome
- Subcellular localizatione1
- Qualifier
- targeted to chloroplastse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e1“Subcellular localization confirmed that GmLOX6 is targeted to chloroplasts”
Claim · model-written summary
GmLOX6 facilitates JA production under salt stress.
- Taxon
- soybeanGlycine maxt0
- Treatment
- salt stresse3
- Gene
- GmLOX6e3
- Outcome
- JA productione3
- Direction
- facilitatese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e3“revealed that GmLOX6 facilitates JA production under salt stress”
Claim · model-written summary
The elevation in JA levels was associated with improved salt tolerance in soybean.
- Taxon
- soybeanGlycine maxt0
- Compound
- JAe4
- Outcome
- salt tolerancee4
- Direction
- improvede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e4“This elevation in JA levels was associated with improved salt tolerance”
Claim · model-written summary
Salt stress increased Na+ extrusion in soybean with enhanced GmLOX6 function.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Na + extrusione5
- Qualifier
- enhancede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e5“enhanced Na + extrusion to preserve ionic balance”
Claim · model-written summary
Activation of antioxidant enzymes detoxified reactive oxygen species in soybean under salt stress.
- Taxon
- soybeanGlycine maxt0
- Outcome
- detoxification of reactive oxygen speciese6
- Qualifier
- activation of antioxidant enzymese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e6“including the activation of antioxidant enzymes for the detoxification of reactive oxygen species (ROS)”
Claim · model-written summary
GmLOX6 reinforces membrane stability in soybean under salt stress.
- Taxon
- soybeanGlycine maxt0
- Outcome
- membrane stabilitye7
- Qualifier
- reinforcede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e7“reinforced membrane stability”
Claim · model-written summary
GmRWP-RK11 is a transcriptional repressor of GmLOX6.
- Taxon
- soybeanGlycine maxt0
- Gene
- GmRWP‐RK11e7
- Outcome
- transcriptional repressor of GmLOX6e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e7“GmRWP‐RK11 was identified as a transcriptional repressor of GmLOX6”
Claim · model-written summary
Functional disruption of GmRWP-RK11 by CRISPR/Cas9 conferred greater salt tolerance in soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Functional disruption of GmRWP‐RK11 via CRISPR/Cas9e7
- Gene
- GmRWP‐RK11e7
- Outcome
- salt tolerancee7
- Direction
- conferred greatere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“salt stress tolerance in soybean”
e7“Functional disruption of GmRWP‐RK11 via CRISPR/Cas9 conferred greater salt tolerance”
The same questions in other papers
salinity · yield · Glycine max (soybean)Results differ
2 papers in the same direction as this paper · 42 in the opposite direction
- negative
- negative
- negative
41 papers more in this group
See all 45 papers →loss of function · tolerance: abiotic · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.