Paper · 2025 · International Journal of Phytoremediation

Zinc sulfide nanoparticles regulate cadmium toxicity in maize ( Zea mays L.) by improving Cd/Zn homeostasis

Laiba Asghar; Almas Jahan; Fahad Shafiq; et al.

13
claims
29
verbatim quotes
0
citations
1
research areas
✓
Open access · cc-by

Abstract

) using completely randomized design (CRD). After 20 d of nZnS supplementation, growth and biochemical responses were recorded. The study highlights the critical issue of Cd stress in maize and investigates how nZnS influences plant tolerance mechanisms. We explored the novelty of application of RZ and FS of nZnS, focusing on its role in Cd/Zn homeostasis, growth and stress resilience. The RZ supplementation with nZnS recovered growth and enhanced maize protein and phenolic content under Cd stress. Exogenous application of nZnS improved chlorophyll under Cd stress. Whereas, exposure to Cd stress markedly reduced Zn in shoots (0.0015 mg/g DW; -99.1%) compared to the control (0.186 mg/g DW). The nZnS application enhanced Zn fraction in shoots (2.03 mg/g DW by RZ; and 1.46 mg/g DW by FS), especially in Cd-stressed plants (2.68 mg/g DW by RZ; and 2.36 mg/g DW) by FS. Exposure to Cd stress resulted in an increased shoot Cd fraction (0.376 mg/g DW), which was further increased by root zone nZnS supply (0.673 mg/g DW; +44.0% compared to Cd-stressed plants. Foliar application of nZnS also enhanced the Cd concentration in the shoots (0.579 mg/g DW; +35.0% compared to the Cd-stressed plants). Although the Cd fraction was synergistically increased due to nZnS supply which can be explained based on Cd/Zn homeostasis reinforced by sulfur (S) supply. In conclusion, the use of nZnS alleviated Cd stress in maize, suggesting that it is an effective strategy for improving Cd tolerance in crops like maize.

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Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Root-zone nZnS supplementation recovered growth of maize under Cd stress.

Taxon
maizeZea mayse1
Treatment
RZ supplementation with nZnSe1
Outcome
growthe1
Direction
recoverede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e1

“The RZ supplementation with nZnS recovered growth and enhanced maize protein and phenolic content under Cd stress”

✓ located verbatim in the source · abstract, characters 389–502 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Root-zone nZnS supplementation enhanced protein and phenolic content of maize under Cd stress.

Taxon
maizeZea mayse1
Treatment
RZ supplementation with nZnSe1
Outcome
protein and phenolic contente1
Direction
enhancede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e1

“The RZ supplementation with nZnS recovered growth and enhanced maize protein and phenolic content under Cd stress”

✓ located verbatim in the source · abstract, characters 389–502 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Exogenous nZnS improved chlorophyll under Cd stress.

Taxon
maizeZea mayst0
Treatment
Exogenous application of nZnSe2
Outcome
chlorophylle2
Direction
improvede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e2

“Exogenous application of nZnS improved chlorophyll under Cd stress”

✓ located verbatim in the source · abstract, characters 504–570 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cd stress markedly reduced Zn in maize shoots compared with the control.

Taxon
maizeZea mayst0
Treatment
Cd stresse3
Outcome
Zn in shootse3
Direction
reducede3
Value
0.0015 mg/g DW; -99.1%e3
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e3

“exposure to Cd stress markedly reduced Zn in shoots (0.0015 mg/g DW; -99.1%) compared to the control (0.186 mg/g DW)”

✓ located verbatim in the source · abstract, characters 581–697 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Root-zone nZnS enhanced the shoot Zn fraction of maize.

Taxon
maizeZea mayst0
Treatment
nZnS applicatione4
Outcome
Zn fraction in shootse4
Direction
enhancede4
Value
2.03 mg/g DWe4
Qualifier
by RZe4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e4

“The nZnS application enhanced Zn fraction in shoots (2.03 mg/g DW by RZ; and 1.46 mg/g DW by FS)”

✓ located verbatim in the source · abstract, characters 699–795 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar nZnS enhanced the shoot Zn fraction of maize.

Taxon
maizeZea mayst0
Treatment
nZnS applicatione4
Outcome
Zn fraction in shootse4
Direction
enhancede4
Value
1.46 mg/g DWe4
Qualifier
by FSe4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e4

“The nZnS application enhanced Zn fraction in shoots (2.03 mg/g DW by RZ; and 1.46 mg/g DW by FS)”

✓ located verbatim in the source · abstract, characters 699–795 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

In Cd-stressed maize, root-zone nZnS raised the shoot Zn fraction to 2.68 mg/g DW.

Taxon
maizeZea mayst0
Outcome
Zn fraction in shootse4
Value
2.68 mg/g DWe5
Qualifier
Cd-stressed plantse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e4

“The nZnS application enhanced Zn fraction in shoots (2.03 mg/g DW by RZ; and 1.46 mg/g DW by FS)”

✓ located verbatim in the source · abstract, characters 699–795 · show in text ↑
e5

“especially in Cd-stressed plants (2.68 mg/g DW by RZ; and 2.36 mg/g DW) by FS”

✓ located verbatim in the source · abstract, characters 797–874 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

In Cd-stressed maize, foliar nZnS raised the shoot Zn fraction to 2.36 mg/g DW.

Taxon
maizeZea mayst0
Outcome
Zn fraction in shootse4
Value
2.36 mg/g DWe5
Qualifier
Cd-stressed plantse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e4

“The nZnS application enhanced Zn fraction in shoots (2.03 mg/g DW by RZ; and 1.46 mg/g DW by FS)”

✓ located verbatim in the source · abstract, characters 699–795 · show in text ↑
e5

“especially in Cd-stressed plants (2.68 mg/g DW by RZ; and 2.36 mg/g DW) by FS”

✓ located verbatim in the source · abstract, characters 797–874 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cd stress increased the shoot Cd fraction of maize.

Taxon
maizeZea mayst0
Treatment
Cd stresse6
Outcome
shoot Cd fractione6
Direction
increasede6
Value
0.376 mg/g DWe6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e6

“Exposure to Cd stress resulted in an increased shoot Cd fraction (0.376 mg/g DW)”

✓ located verbatim in the source · abstract, characters 876–956 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Root-zone nZnS further increased the shoot Cd fraction compared with Cd-stressed plants.

Taxon
maizeZea mayst0
Treatment
root zone nZnS supplye7
Outcome
shoot Cd fractione6
Direction
further increasede7
Value
0.673 mg/g DW; +44.0%e7
Comparator
Cd-stressed plantse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e6

“Exposure to Cd stress resulted in an increased shoot Cd fraction (0.376 mg/g DW)”

✓ located verbatim in the source · abstract, characters 876–956 · show in text ↑
e7

“which was further increased by root zone nZnS supply (0.673 mg/g DW; +44.0% compared to Cd-stressed plants”

✓ located verbatim in the source · abstract, characters 958–1064 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Foliar nZnS enhanced the shoot Cd concentration compared with Cd-stressed plants.

Taxon
maizeZea mayst0
Treatment
Foliar application of nZnSe1
Outcome
Cd concentration in the shootse1
Direction
enhancede1
Value
0.579 mg/g DW; +35.0%e1
Comparator
Cd-stressed plantse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e1

“Foliar application of nZnS also enhanced the Cd concentration in the shoots (0.579 mg/g DW; +35.0% compared to the Cd-stressed plants)”

✓ located verbatim in the source · abstract, characters 1066–1200 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The synergistic increase in Cd fraction under nZnS supply is explained by Cd/Zn homeostasis reinforced by sulfur supply.

Taxon
maizeZea mayst0
Treatment
nZnS supplye1
Outcome
Cd fractione1
Qualifier
Cd/Zn homeostasis reinforced by sulfur (S) supplye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e1

“Although the Cd fraction was synergistically increased due to nZnS supply which can be explained based on Cd/Zn homeostasis reinforced by sulfur (S) supply”

✓ located verbatim in the source · abstract, characters 1202–1357 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The use of nZnS is an effective strategy for improving Cd tolerance in maize.

Taxon
maizeZea mayse1
Treatment
the use of nZnSe1
Outcome
improving Cd tolerancee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cadmium toxicity in maize”

✓ located verbatim in the source · title, characters 36–61 · show in text ↑
e1

“the use of nZnS alleviated Cd stress in maize, suggesting that it is an effective strategy for improving Cd tolerance in crops like maize”

✓ located verbatim in the source · abstract, characters 1374–1511 · show in text ↑

cadmium atom · nutrient: content · Zea mays (maize)Positive and negative results

46 papers: 6 positive, 40 negative or no effect

39 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

42 papers more in this group

See all 46 papers →

application · nutrient: content · Zea mays (maize)Positive and negative results

161 papers: 151 positive, 10 negative or no effect

9 papers in the same direction as this paper · 151 in the opposite direction

  1. positive
  2. positive
  3. positive

157 papers more in this group

See all 161 papers →

heavy metal · nutrient: content · Zea mays (maize)Results agree

13 papers: 0 positive, 13 negative or no effect

12 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.