Paper · 2025 · Scientific Reports

Integrating ryegrass-maize intercropping systems for mitigating Cd-Pb contamination: enhancing ecosystem services and habitat sustainability

Shenglan Ye; Yan Niu; Bei Zhang

12
claims
27
verbatim quotes
2
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

In agricultural lands, cadmium (Cd) and lead (Pb) co-contamination poses a significant threat to soil health and crop safety. This study evaluates the ryegrass-maize intercropping system as a sustainable approach to mitigate these environmental challenges. A 120-day pot experiment was conducted, revealing that the intercropping system significantly reduced soil Cd and Pb, achieving residual levels below national risk thresholds. Maize grains were confirmed safe, while ryegrass acted as an efficient bioaccumulator. The system enhanced soil ecosystem services, evidenced by a significant increase in key soil enzyme activities and nutrient cycling indicators. Specifically, the intercropping system boosted the activities of catalase, alkaline protease, and urease, which are crucial for redox regulation and nitrogen cycling. Notably, the activity of alkaline protease, responsible for organic nitrogen decomposition, increased by approximately 18.5 times in the intercropping group. Furthermore, the system increased soil organic matter by 5.89%, a core indicator of soil fertility and carbon sequestration, a key supporting service. Habitat sustainability was substantiated through the analysis of microbial community structure and diversity. Although Cd-Pb contamination initially reduced microbial diversity, the intercropping system (C2) mitigated this decline more effectively than monoculture (C1). More importantly, the intercropping system enriched metal-tolerant and functionally critical microbial taxa, such as Actinobacteriota, whose relative abundance reached 43.99% in the C2 group after the experiment. Despite observed phosphorus depletion, which suggests the need for targeted fertilization, the findings support ryegrass-maize intercropping as a viable, nature-based solution for soil remediation. This scalable strategy not only addresses contamination but also supports sustainable land management, contributing to the resilience and restoration of degraded farmlands.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Ryegrass-maize intercropping significantly reduced soil Cd and Pb in a pot experiment.

Taxon
maizeZea mayse0
Treatment
intercropping systeme1
Outcome
soil Cd and Pbe1
Direction
reducede1
Setting
pot experimente2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e1

“the intercropping system significantly reduced soil Cd and Pb, achieving residual levels below national risk thresholds”

✓ located verbatim in the source · abstract, characters 312–431 · show in text ↑
e2

“A 120-day pot experiment was conducted”

✓ located verbatim in the source · abstract, characters 257–295 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Maize grains were confirmed safe for consumption.

Taxon
maizeZea mayse0
Organ / stage
grainse3
Outcome
safee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e3

“Maize grains were confirmed safe, while ryegrass acted as an efficient bioaccumulator”

✓ located verbatim in the source · abstract, characters 433–518 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Ryegrass acted as an efficient bioaccumulator in the intercropping system.

Taxon
ryegrassLoliume0
Outcome
bioaccumulatore3
Qualifier
efficiente3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lolium
Lolium · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e3

“Maize grains were confirmed safe, while ryegrass acted as an efficient bioaccumulator”

✓ located verbatim in the source · abstract, characters 433–518 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Intercropping significantly increased key soil enzyme activities.

Taxon
maizeZea mayse0
Treatment
intercropping systeme1
Outcome
key soil enzyme activitiese4
Direction
increasee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e1

“the intercropping system significantly reduced soil Cd and Pb, achieving residual levels below national risk thresholds”

✓ located verbatim in the source · abstract, characters 312–431 · show in text ↑
e4

“The system enhanced soil ecosystem services, evidenced by a significant increase in key soil enzyme activities”

✓ located verbatim in the source · abstract, characters 520–630 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping boosted the activities of catalase, alkaline protease and urease in soil.

Taxon
maizeZea mayse0
Treatment
intercropping systeme5
Outcome
activities of catalase, alkaline protease, and ureasee5
Direction
boostede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e5

“Specifically, the intercropping system boosted the activities of catalase, alkaline protease, and urease”

✓ located verbatim in the source · abstract, characters 664–768 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Alkaline protease activity increased about 18.5 times under intercropping.

Taxon
maizeZea mayse0
Treatment
intercropping groupe6
Outcome
activity of alkaline proteasee6
Direction
increasede6
Value
approximately 18.5 timese6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e6

“the activity of alkaline protease, responsible for organic nitrogen decomposition, increased by approximately 18.5 times in the intercropping group”

✓ located verbatim in the source · abstract, characters 840–987 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping increased soil organic matter by 5.89%.

Taxon
maizeZea mayse0
Treatment
intercropping systeme1
Outcome
soil organic mattere7
Direction
increasede7
Value
5.89%e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e1

“the intercropping system significantly reduced soil Cd and Pb, achieving residual levels below national risk thresholds”

✓ located verbatim in the source · abstract, characters 312–431 · show in text ↑
e7

“the system increased soil organic matter by 5.89%”

✓ located verbatim in the source · abstract, characters 1002–1051 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cd-Pb contamination initially reduced soil microbial diversity.

Taxon
maizeZea mayse0
Treatment
Cd-Pb contaminatione5
Outcome
microbial diversitye5
Direction
reducede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e5

“Although Cd-Pb contamination initially reduced microbial diversity, the intercropping system (C2) mitigated this decline more effectively than monoculture (C1)”

✓ located verbatim in the source · abstract, characters 1250–1409 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping mitigated the decline in microbial diversity more effectively than monoculture.

Taxon
maizeZea mayse0
Treatment
intercropping system (C2)e5
Outcome
microbial diversitye5
Direction
mitigatede5
Comparator
monoculture (C1)e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e5

“Although Cd-Pb contamination initially reduced microbial diversity, the intercropping system (C2) mitigated this decline more effectively than monoculture (C1)”

✓ located verbatim in the source · abstract, characters 1250–1409 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Intercropping enriched metal-tolerant and functionally critical microbial taxa, such as Actinobacteriota, whose relative abundance reached 43.99% in the C2 group.

Taxon
maizeZea mayse0
Treatment
intercropping systeme4
Outcome
relative abundancee4
Direction
enrichede4
Value
43.99%e4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e4

“the intercropping system enriched metal-tolerant and functionally critical microbial taxa, such as Actinobacteriota, whose relative abundance reached 43.99% in the C2 group after the experiment”

✓ located verbatim in the source · abstract, characters 1429–1622 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Observed phosphorus depletion occurred in the Cd-Pb co-contaminated soil under intercropping.

Taxon
maizeZea mayse0
Outcome
phosphoruse3
Direction
depletione3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e3

“Despite observed phosphorus depletion”

✓ located verbatim in the source · abstract, characters 1624–1661 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Ryegrass-maize intercropping is a viable, nature-based solution for soil remediation.

Taxon
ryegrassLoliume3
Outcome
soil remediatione3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lolium
Lolium · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study evaluates the ryegrass-maize intercropping system”

✓ located verbatim in the source · abstract, characters 126–186 · show in text ↑
e3

“the findings support ryegrass-maize intercropping as a viable, nature-based solution for soil remediation”

✓ located verbatim in the source · abstract, characters 1715–1820 · show in text ↑

cadmium atom · soil: biology · Zea mays (maize)Positive and negative results

6 papers: 1 positive, 5 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

2 papers more in this group

See all 6 papers →

intercropping · physiology: antioxidant enzymes · Zea mays (maize)Positive and negative results

7 papers: 6 positive, 1 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

3 papers more in this group

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intercropping · pest · Zea mays (maize)Positive and negative results

38 papers: 31 positive, 7 negative or no effect

30 papers in the same direction as this paper · 7 in the opposite direction

  1. negative
  2. no effect
  3. no effect

34 papers more in this group

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intercropping · soil: chemistry · Zea mays (maize)Positive and negative results

42 papers: 37 positive, 5 negative or no effect

36 papers in the same direction as this paper · 3 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

38 papers more in this group

See all 42 papers →

intercropping · nutrient: content · Zea mays (maize)Positive and negative results

141 papers: 122 positive, 19 negative or no effect

121 papers in the same direction as this paper · 15 in the opposite direction · 4 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

137 papers more in this group

See all 141 papers →

intercropping · soil: biology · Zea mays (maize)Results agree

27 papers: 27 positive, 0 negative or no effect

26 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

23 papers more in this group

See all 27 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.