Paper · 2009 · American-Asian-Journal of agricultural & environmental sciences/American-Eurasian journal of agricultural & environmental sciences

The effects of carbohydrate source and concentration on somatic embryogenesis of strawberry (Fragaria × ananassa Duch.).

Mohammad Gerdakaneh; Ali-Akbar Mozafari; A Khalighi; et al.

8
claims
16
verbatim quotes
19
citations
2
research areas
—
Not open access

Abstract

This experiment was performed to evaluate the effects of different types and concentrations of carbohydrate source on callus fresh weight, number of somatic embryos per embryonic explant and percentage of globular embryos developing into cotyledonary embryos from the leaf explants of three strawberry cultivars (Kurdistan, Paros and Camarosa). MS medium containing 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l 6-benzyladenine (BA) supplemented with three types of carbohydrates (sucrose, glucose and fructose) at concentration of 1.5%, 3%, 6%, 9% and 12% separately were tested. Among the different sugars tested, 6% sucrose was found superior not only for giving optimum embryo induction of embryonic culture but also a uniform embryo developmental stages. Maximum number of 11.68, 12.68 and 13.35 globular embryos per explant, 81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively. Sucrose also was the best for proliferation of embryonic tissue with an optimal concentration of 3%.

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Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Six-percent sucrose gave optimal embryo induction and uniform embryo stages.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Treatment
6% sucrosee0
Outcome
optimum embryo induction of embryonic culture but also a uniform embryo developmental stagese0
Direction
superiore0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“6% sucrose was found superior not only for giving optimum embryo induction of embryonic culture but also a uniform embryo developmental stages”

✓ located verbatim in the source · abstract, characters 631–773 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Kurdistan produced 11.68 globular embryos per explant.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Kurdistane0
Outcome
globular embryos per explante0
Direction
Maximume0
Value
11.68e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum number of 11.68, 12.68 and 13.35 globular embryos per explant, 81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 775–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Paros produced 12.68 globular embryos per explant.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Parose0
Outcome
globular embryos per explante0
Direction
Maximume0
Value
12.68e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum number of 11.68, 12.68 and 13.35 globular embryos per explant, 81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 775–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Camarosa produced 13.35 globular embryos per explant.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Camarosae0
Outcome
globular embryos per explante0
Direction
Maximume0
Value
13.35e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum number of 11.68, 12.68 and 13.35 globular embryos per explant, 81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 775–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Kurdistan had 81.53% globular-to-cotyledonary embryo development.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Kurdistane0
Outcome
globular embryos developing into cotyledonary embryose0
Value
81.53e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 846–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Paros had 87.65% globular-to-cotyledonary embryo development.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Parose0
Outcome
globular embryos developing into cotyledonary embryose0
Value
87.65e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 846–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Camarosa had 86.35% globular-to-cotyledonary embryo development.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Material
Camarosae0
Outcome
globular embryos developing into cotyledonary embryose0
Value
86.35%e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“81.53, 87.65 and 86.35% of globular embryos developing into cotyledonary embryos obtained for the cultivars of Kurdistan, Paros and Camarosa, respectively”

✓ located verbatim in the source · abstract, characters 846–1000 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Three-percent sucrose was optimal for embryonic tissue proliferation.

Taxon
strawberry (Fragaria × ananassa Duch.)Fragaria ×ananassat0
Treatment
3%e0
Outcome
proliferation of embryonic tissuee0
Direction
beste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Sucrose also was the best for proliferation of embryonic tissue with an optimal concentration of 3%”

✓ located verbatim in the source · abstract, characters 1002–1101 · show in text ↑
t0

“strawberry (Fragaria × ananassa Duch.)”

✓ located verbatim in the source · title, characters 81–119 · show in text ↑

sucrose · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.