Abstract
Strawberry is an extensively planted horticulture crop with multiple economic values. Vacuolar phosphate transporter (FaVPT1) can promote phosphate and sugar accumulation and improve strawberry fruit quality, however, its transcription regulatory mechanism remains unknown. Here, we report a GRAS superfamily transcription factor (TF) SCARECROW-LIKE8 (FaSCL8) which regulates FaVPT1 expression to control strawberry fruit ripening and quality. The promoter of FaVPT1 was used as a bait to screen the octoploid strawberry cDNA library by yeast one hybrid to obtain the candidate TFs including FaSCL8. FaSCL8 was located in the nucleus and was mainly expressed in fruit, its expression level was increased rapidly during ripening, and induced by exogenous phytohormones including ABA, GA, IAA, and ethylene, as well as sucrose. FaSCL8 can directly combine with the FaVPT1 promoter and regulate its expression in vitro and in vivo. The results of Agrobacterium-mediated transient infection of strawberry fruits showed that overexpression of FaSCL8 could increase soluble sugar, anthocyanin, and phosphorus content, promoting fruit ripening, while decreasing expression of FaSCL8 showed the opposite phenotype. The expression levels of fruit ripening and quality-related genes including FaVPT1, were improved in the over-expressed fruits of FaSCL8, while receded in the FaSCL8-silenced fruits. In conclusion, this study found that FaSCL8 can regulate FaVPT1 expression to improve phosphorus and sugar accumulation in strawberry fruits and promote fruit ripening, providing a new regulation mechanism of strawberry fruit ripening by phosphorus, a macroelement key to fruit quality improvement.
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Claims 12
Claim · model-written summary
FaSCL8 was identified among candidate transcription factors binding the FaVPT1 promoter.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- FaSCL8e0
- Outcome
- candidate TFs including FaSCL8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The promoter of FaVPT1 was used as a bait to screen the octoploid strawberry cDNA library by yeast one hybrid to obtain the candidate TFs including FaSCL8”
Claim · model-written summary
FaSCL8 was nuclear and mainly expressed in fruit.
- Taxon
- strawberryFragaria ×ananassae1
- Organ / stage
- fruite0
- Gene
- FaSCL8e0
- Outcome
- located in the nucleus and was mainly expressed in fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaSCL8 was located in the nucleus and was mainly expressed in fruit”
e1“octoploid strawberry cDNA library”
Claim · model-written summary
FaSCL8 expression rose rapidly during ripening.
- Taxon
- strawberryFragaria ×ananassae1
- Organ / stage
- ripeninge0
- Gene
- FaSCL8e2
- Outcome
- expression levele0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression level was increased rapidly during ripening”
e1“octoploid strawberry cDNA library”
e2“FaSCL8 was located in the nucleus”
Claim · model-written summary
ABA, GA and IAA induced FaSCL8 expression.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- exogenous phytohormones including ABA, GA, IAAe0
- Gene
- FaSCL8e2
- Outcome
- expression levele3
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced by exogenous phytohormones including ABA, GA, IAA, and ethylene, as well as sucrose”
e1“octoploid strawberry cDNA library”
e2“FaSCL8 was located in the nucleus”
e3“its expression level was increased rapidly during ripening”
Claim · model-written summary
Ethylene induced FaSCL8 expression.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- ethylenee0
- Gene
- FaSCL8e2
- Outcome
- expression levele3
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced by exogenous phytohormones including ABA, GA, IAA, and ethylene, as well as sucrose”
e1“octoploid strawberry cDNA library”
e2“FaSCL8 was located in the nucleus”
e3“its expression level was increased rapidly during ripening”
Claim · model-written summary
Sucrose induced FaSCL8 expression.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- sucrosee0
- Gene
- FaSCL8e2
- Outcome
- expression levele3
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced by exogenous phytohormones including ABA, GA, IAA, and ethylene, as well as sucrose”
e1“octoploid strawberry cDNA library”
e2“FaSCL8 was located in the nucleus”
e3“its expression level was increased rapidly during ripening”
Claim · model-written summary
FaSCL8 directly bound the FaVPT1 promoter and regulated its expression in vitro and in vivo.
- Taxon
- strawberryFragaria ×ananassae1
- Gene
- FaSCL8e0
- Outcome
- FaVPT1 promotere0
- Qualifier
- in vitro and in vivoe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaSCL8 can directly combine with the FaVPT1 promoter and regulate its expression in vitro and in vivo”
e1“octoploid strawberry cDNA library”
Claim · model-written summary
FaSCL8 overexpression increased soluble sugar, anthocyanin and phosphorus content.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- overexpression of FaSCL8e0
- Gene
- FaSCL8e0
- Outcome
- soluble sugar, anthocyanin, and phosphorus contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of FaSCL8 could increase soluble sugar, anthocyanin, and phosphorus content”
e1“octoploid strawberry cDNA library”
Claim · model-written summary
FaSCL8 overexpression promoted fruit ripening.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- overexpression of FaSCL8e2
- Gene
- FaSCL8e0
- Outcome
- fruit ripeninge0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting fruit ripening, while decreasing expression of FaSCL8 showed the opposite phenotype”
e1“octoploid strawberry cDNA library”
e2“overexpression of FaSCL8 could increase”
Claim · model-written summary
Reducing FaSCL8 expression gave the opposite fruit phenotype to overexpression.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- decreasing expression of FaSCL8e0
- Gene
- FaSCL8e0
- Outcome
- opposite phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreasing expression of FaSCL8 showed the opposite phenotype”
e1“octoploid strawberry cDNA library”
Claim · model-written summary
FaSCL8 overexpression raised expression of fruit ripening and quality genes including FaVPT1.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- over-expressed fruits of FaSCL8e0
- Gene
- FaVPT1e0
- Outcome
- expression levels of fruit ripening and quality-related genese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels of fruit ripening and quality-related genes including FaVPT1, were improved in the over-expressed fruits of FaSCL8”
e1“octoploid strawberry cDNA library”
Claim · model-written summary
FaSCL8 silencing reduced expression of fruit ripening and quality genes.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- FaSCL8-silenced fruitse0
- Gene
- FaSCL8e0
- Outcome
- expression levels of fruit ripening and quality-related genese2
- Direction
- recedede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“receded in the FaSCL8-silenced fruits”
e1“octoploid strawberry cDNA library”
e2“The expression levels of fruit ripening and quality-related genes”
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