Paper · 2011 · Acta Horticulturae Sinica

Expression changes of ABA receptor gene FaABAR/CHLH in strawberry and its affection factors.

Haifeng Jia; Yemao Chai; Chunli Li; et al.

9
claims
16
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

To investigate the signalling perception mechanism of ABA regulation on strawberry fruit ripening,experiment with'Beinongxiang'strawberry fruit during 1-4 weeks after anthesis,the mRNA expression level of FaABAR/CHLH gene in developing fruit and its affection factors were detected via real-time PCR and fruit-tissue discs.The results showed that a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily.During the early developmental stage of frui(tsmall green,large green,and light green),the FaABAR/CHLH gene expressed highly.Coupled with fruit de-greening,the transcription level of the gene rapidly decreased,and then up to the lowest level at white stage.On the beginning of fruit red-coloring,the transcription level of the gene rapidly increased.Both ABA and pH could markedly promote the mRNA expression level of the gene.In contrast,sucrosesignificantly inhibited the gene transcripts.The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening.

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

A 4 325 bp cDNA encoded a 1 381 amino acid strawberry ABA receptor protein.

Taxon
strawberryFragaria ×ananassae0
Gene
FaABAR/CHLHe0
Outcome
encoded a strawberry ABA receptor FaABAR/CHLH proteine0
Value
4 325 bpe0
Qualifier
1 381 amino acid sequencese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily”

✓ located verbatim in the source · abstract, characters 347–534 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaABAR/CHLH had a conserved metal ion chelatase H subunit domain.

Taxon
strawberryFragaria ×ananassat0
Gene
FaABAR/CHLHe0
Outcome
conserved domain of metal ion chelatase H subunit superfamilye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily”

✓ located verbatim in the source · abstract, characters 406–534 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

FaABAR/CHLH expression was high in early fruit developmental stages.

Taxon
strawberryFragaria ×ananassat0
Organ / stage
early developmental stagee0
Gene
FaABAR/CHLHe0
Outcome
FaABAR/CHLH gene expressed highlye0
Direction
highlye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“During the early developmental stage of frui(tsmall green,large green,and light green),the FaABAR/CHLH gene expressed highly”

✓ located verbatim in the source · abstract, characters 535–659 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

FaABAR/CHLH transcription declined with fruit de-greening to its minimum at white stage.

Taxon
strawberryFragaria ×ananassat0
Organ / stage
white stagee0
Outcome
transcription level of the genee0
Direction
decreasede0
Qualifier
fruit de-greeninge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Coupled with fruit de-greening,the transcription level of the gene rapidly decreased,and then up to the lowest level at white stage”

✓ located verbatim in the source · abstract, characters 660–791 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gene transcription rose rapidly at the start of fruit red coloring.

Taxon
strawberryFragaria ×ananassat0
Organ / stage
fruit red-coloringe0
Outcome
transcription level of the genee0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“On the beginning of fruit red-coloring,the transcription level of the gene rapidly increased”

✓ located verbatim in the source · abstract, characters 792–884 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ABA markedly promoted gene mRNA expression.

Taxon
strawberryFragaria ×ananassat0
Treatment
ABAe0
Outcome
mRNA expression level of the genee0
Direction
promotee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both ABA and pH could markedly promote the mRNA expression level of the gene”

✓ located verbatim in the source · abstract, characters 885–961 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

pH markedly promoted gene mRNA expression.

Taxon
strawberryFragaria ×ananassat0
Treatment
pHe0
Outcome
mRNA expression level of the genee0
Direction
promotee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both ABA and pH could markedly promote the mRNA expression level of the gene”

✓ located verbatim in the source · abstract, characters 885–961 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Sucrose significantly inhibited gene transcripts.

Taxon
strawberryFragaria ×ananassat0
Treatment
sucrosee0
Outcome
gene transcriptse0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“sucrosesignificantly inhibited the gene transcripts”

✓ located verbatim in the source · abstract, characters 974–1025 · show in text ↑
t0

“strawberry”

✓ located verbatim in the source · title, characters 55–65 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Expression changes suggest a role for ABA in fruit ripening.

Taxon
strawberryFragaria ×ananassae0
Gene
FaABA/CHLHe0
Outcome
regulation of fruit ripeninge0
Qualifier
important role of ABAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening”

✓ located verbatim in the source · abstract, characters 1026–1208 · show in text ↑

sucrose · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

(+)-abscisic acid · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results

10 papers: 8 positive, 2 negative or no effect

7 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.