Abstract
To investigate the signalling perception mechanism of ABA regulation on strawberry fruit ripening,experiment with'Beinongxiang'strawberry fruit during 1-4 weeks after anthesis,the mRNA expression level of FaABAR/CHLH gene in developing fruit and its affection factors were detected via real-time PCR and fruit-tissue discs.The results showed that a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily.During the early developmental stage of frui(tsmall green,large green,and light green),the FaABAR/CHLH gene expressed highly.Coupled with fruit de-greening,the transcription level of the gene rapidly decreased,and then up to the lowest level at white stage.On the beginning of fruit red-coloring,the transcription level of the gene rapidly increased.Both ABA and pH could markedly promote the mRNA expression level of the gene.In contrast,sucrosesignificantly inhibited the gene transcripts.The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening.
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Claims 9
Claim · model-written summary
A 4 325 bp cDNA encoded a 1 381 amino acid strawberry ABA receptor protein.
- Taxon
- strawberry
Fragaria ×ananassae0 - Gene
- FaABAR/CHLHe0
- Outcome
- encoded a strawberry ABA receptor FaABAR/CHLH proteine0
- Value
- 4 325 bpe0
- Qualifier
- 1 381 amino acid sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily”
Claim · model-written summary
FaABAR/CHLH had a conserved metal ion chelatase H subunit domain.
- Taxon
- strawberry
Fragaria ×ananassat0 - Gene
- FaABAR/CHLHe0
- Outcome
- conserved domain of metal ion chelatase H subunit superfamilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily”
t0“strawberry”
Claim · model-written summary
FaABAR/CHLH expression was high in early fruit developmental stages.
- Taxon
- strawberry
Fragaria ×ananassat0 - Organ / stage
- early developmental stagee0
- Gene
- FaABAR/CHLHe0
- Outcome
- FaABAR/CHLH gene expressed highlye0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“During the early developmental stage of frui(tsmall green,large green,and light green),the FaABAR/CHLH gene expressed highly”
t0“strawberry”
Claim · model-written summary
FaABAR/CHLH transcription declined with fruit de-greening to its minimum at white stage.
- Taxon
- strawberry
Fragaria ×ananassat0 - Organ / stage
- white stagee0
- Outcome
- transcription level of the genee0
- Direction
- decreasede0
- Qualifier
- fruit de-greeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Coupled with fruit de-greening,the transcription level of the gene rapidly decreased,and then up to the lowest level at white stage”
t0“strawberry”
Claim · model-written summary
Gene transcription rose rapidly at the start of fruit red coloring.
- Taxon
- strawberry
Fragaria ×ananassat0 - Organ / stage
- fruit red-coloringe0
- Outcome
- transcription level of the genee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“On the beginning of fruit red-coloring,the transcription level of the gene rapidly increased”
t0“strawberry”
Claim · model-written summary
ABA markedly promoted gene mRNA expression.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- ABAe0
- Outcome
- mRNA expression level of the genee0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Both ABA and pH could markedly promote the mRNA expression level of the gene”
t0“strawberry”
Claim · model-written summary
pH markedly promoted gene mRNA expression.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- pHe0
- Outcome
- mRNA expression level of the genee0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Both ABA and pH could markedly promote the mRNA expression level of the gene”
t0“strawberry”
Claim · model-written summary
Sucrose significantly inhibited gene transcripts.
- Taxon
- strawberry
Fragaria ×ananassat0 - Treatment
- sucrosee0
- Outcome
- gene transcriptse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“sucrosesignificantly inhibited the gene transcripts”
t0“strawberry”
Claim · model-written summary
Expression changes suggest a role for ABA in fruit ripening.
- Taxon
- strawberry
Fragaria ×ananassae0 - Gene
- FaABA/CHLHe0
- Outcome
- regulation of fruit ripeninge0
- Qualifier
- important role of ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening”
The same questions in other papers
sucrose · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
(+)-abscisic acid · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.