Abstract
The effects of some selected heavy metals (zinc, copper, cadmium, nickel and control) on DNA content in sunflower plants was investigated using flow cytometry. The LD50 estimated concentration for each of these four heavy metals were applied and tolerant seedlings were selected. The treated and untreated sunflower leaf nuclei were isolated and were assayed using (FACS, Calibur, Becton Dickinson, USA). The obtained profiles represented a typical distribution for a diploid DNA content, where most of the cells (85.81%) grouped at the 2C DNA values of G0 and G1 phases, only few nuclei (1.16%) corresponded to the 4C DNA values of G2 and M phases, the rest (13.04%) represented the S phase with C values ranging between 2C and 4C. According to the present findings, it can be concluded that sunflower plants tolerance to these four heavy metals: i.e. Zn, Cu, Cd, and Ni, do not modify the cell cycle phases or DNA synthesis. Only for Ni-tolerant plants, signs of cell necrosis have been noticed without interference with cell cycle phases. This could be attributed to the physiological and enzymatic role of phytochelatins and related metabolisms in the process of heavy metal tolerance in plants.
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Claims 6
Claim · model-written summary
Most sunflower nuclei were at 2C DNA content in G0/G1.
- Taxon
- sunflower plants
Helianthus annuust0 - Outcome
- 2C DNA values of G0 and G1 phasese0
- Value
- 85.81%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the cells (85.81%) grouped at the 2C DNA values of G0 and G1 phases”
t0“sunflower plants”
Claim · model-written summary
Few sunflower nuclei were at 4C DNA content in G2/M.
- Taxon
- sunflower plantsHelianthus annuust0
- Outcome
- 4C DNA values of G2 and M phasese0
- Value
- 1.16%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only few nuclei (1.16%) corresponded to the 4C DNA values of G2 and M phases”
t0“sunflower plants”
Claim · model-written summary
The remaining sunflower cells were in S phase.
- Taxon
- sunflower plantsHelianthus annuust0
- Outcome
- S phasee0
- Value
- 13.04%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rest (13.04%) represented the S phase with C values ranging between 2C and 4C”
t0“sunflower plants”
Claim · model-written summary
Tolerance to Zn, Cu, Cd and Ni did not modify sunflower cell cycle phases or DNA synthesis.
- Taxon
- sunflower plantsHelianthus annuuse0
- Treatment
- Zn, Cu, Cd, and Nie0
- Outcome
- cell cycle phases or DNA synthesise0
- Direction
- do not modifye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower plants tolerance to these four heavy metals: i.e. Zn, Cu, Cd, and Ni, do not modify the cell cycle phases or DNA synthesis”
Claim · model-written summary
Necrosis signs occurred in Ni-tolerant sunflower plants without cell cycle interference.
- Taxon
- sunflower plantsHelianthus annuust0
- Treatment
- Ni-tolerante0
- Outcome
- signs of cell necrosise0
- Direction
- have been noticede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only for Ni-tolerant plants, signs of cell necrosis have been noticed without interference with cell cycle phases”
t0“sunflower plants”
Claim · model-written summary
Authors suggest phytochelatins and related metabolism may explain heavy metal tolerance.
- Taxon
- sunflower plantsHelianthus annuust0
- Compound
- phytochelatinse0
- Outcome
- heavy metal tolerancee0
- Qualifier
- could be attributede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This could be attributed to the physiological and enzymatic role of phytochelatins and related metabolisms in the process of heavy metal tolerance in plants”
t0“sunflower plants”
The same questions in other papers
zinc atom · molecular: metabolites · Helianthus annuus (common sunflower)Positive and negative results
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See all 7 papers →copper atom · molecular: metabolites · Helianthus annuus (common sunflower)Results agree
2 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.