Abstract
Blueberry ( Vaccinium ashei ) is highly characterized by its nutritional value, with an extremely high anthocyanin content, and rabbiteye blueberry is widely grown across China. However, molecular regulatory mechanisms underlying the high anthocyanin accumulation during the fruit development and colouration of rabbiteye blueberry fruit, have not yet been fully clarified so far. The fruit anthocyanin content of rabbiteye blueberry in the karstic area of Guizhou Province, China, is much higher compared to that in other regions, and the fruit colour is highly affected by anthocyanin accumulation. Currently, the untargeted metabolomics and HPLC assays have been carried out using rabbiteye blueberry fruit at various stages, and it was investigated that cyanidin (Cy) and pelargonidin (Pg) reached their peaks at the red fruit (RF) stage, whereas delphinidin (Dp), petudinin (Pt), malvidin (Mv), and peonidin (Pn) got their ceilings at the mature fruit (MF) stage. Transcriptome and co-expression network analyses showed that 27 differentially expressed genes (DEGs) were associated with anthocyanin content, among which VdMYB56, belonging to the R2R3-MYB family, was markedly up-regulated during the development and colouration of fruit, and was significantly higher in the skin than in the pulp. Furthermore, VdMYB56 -overexpressing tomato fruits demonstrated a substantial elevation in anthocyanin content on the 35 th day after flowering (DAF). It was worth noting that VdMYB56 could directly bind to the promoter of Vd3GT to enhance its expression, thereby further strengthening the anthocyanin accumulation. Meantime, multiple assays verified that VdMYB69, an R2R3-MYB member, might interact with VdMYB56, leading to the promotion of VdMYB56 expression. Conclusively, the VdMYB56-VdMYB69 module is a positive regulator of anthocyanin biosynthesis in rabbiteye blueberry, which may provide new insights into high-anthocyanin breeding, particularly for the southern karstic regions.
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Claims 10
Claim · model-written summary
Fruit anthocyanin content was higher in Guizhou karst than other regions.
Verbatim
e0“The fruit anthocyanin content of rabbiteye blueberry in the karstic area of Guizhou Province, China, is much higher compared to that in other regions”
Claim · model-written summary
Fruit colour was strongly affected by anthocyanin accumulation.
Not reportedDirection
Verbatim
e0“the fruit colour is highly affected by anthocyanin accumulation”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
Cyanidin and pelargonidin peaked at the red fruit stage.
Verbatim
e0“cyanidin (Cy) and pelargonidin (Pg) reached their peaks at the red fruit (RF) stage”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
Delphinidin, petudinin, malvidin and peonidin peaked at mature fruit stage.
Verbatim
e0“delphinidin (Dp), petudinin (Pt), malvidin (Mv), and peonidin (Pn) got their ceilings at the mature fruit (MF) stage”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
Twenty-seven differentially expressed genes were associated with anthocyanin content.
- Taxon
- rabbiteye blueberryt0
- Gene
- differentially expressed genes (DEGs)e0
- Outcome
- anthocyanin contente0
- Value
- 27e0
Not reportedDirection
Verbatim
e0“27 differentially expressed genes (DEGs) were associated with anthocyanin content”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
VdMYB56 was upregulated during fruit development and colouration.
Verbatim
e0“VdMYB56, belonging to the R2R3-MYB family, was markedly up-regulated during the development and colouration of fruit”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
VdMYB56 expression was higher in skin than pulp.
Verbatim
e0“was significantly higher in the skin than in the pulp”
e1“VdMYB56, belonging to the R2R3-MYB family”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
VdMYB56 overexpression increased anthocyanin in tomato fruit at 35 days after flowering.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- tomato fruitse0
- Treatment
- VdMYB56 -overexpressinge0
- Gene
- VdMYB56e0
- Outcome
- anthocyanin contente0
- Direction
- elevatione0
- Qualifier
- 35 th day after flowering (DAF)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VdMYB56 -overexpressing tomato fruits demonstrated a substantial elevation in anthocyanin content on the 35 th day after flowering (DAF)”
Claim · model-written summary
VdMYB56 bound the Vd3GT promoter and enhanced its expression and anthocyanin accumulation.
Verbatim
e0“VdMYB56 could directly bind to the promoter of Vd3GT to enhance its expression, thereby further strengthening the anthocyanin accumulation”
t0“rabbiteye blueberry (Vaccinium ashei)”
Claim · model-written summary
VdMYB69 may interact with VdMYB56 and promote VdMYB56 expression.
Verbatim
e0“VdMYB69, an R2R3-MYB member, might interact with VdMYB56, leading to the promotion of VdMYB56 expression”
t0“rabbiteye blueberry (Vaccinium ashei)”