Paper · 2026 · Food Science & Nutrition

Synergistic Effect of Organic Waste Products and Microbial Inocula on Iron and Zinc Biofortification in Cowpea [ Vigna unguiculata (L.) Walp.]

Emmanuel Noumsi‐Foamouhoue; Samuel Legros; Paula Fernandes; et al.

8
claims
10
verbatim quotes
0
citations
2
research areas
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Open access · cc-by

Abstract

ABSTRACT Micronutrient deficiencies affect over 2 billion people worldwide, with iron (Fe) and zinc (Zn) deficiencies prevalent in Senegal. These deficiencies result from the low Fe and Zn contents in food crop products. This study examined the effects of micronutrient‐rich organic waste products (OWPs), in combination with local beneficial microorganisms and mycorrhizal fungi, on Fe and Zn concentrations in cowpea ( Vigna unguiculata ) grains and haulms. Cowpea trials were conducted over two contrasting consecutive seasons (wet and dry). A factorial block design with four replications was used. The main results showed significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control. In addition, the observed effects on the yield and Fe and Zn concentrations depended on the type of OWPs and MIs used. These results validate our initial hypothesis regarding the significant increase in Fe and Zn content in cowpea grains and haulms with the combined application of OWPs, which supply micronutrients, and MIs that facilitate the solubilization and transfer of these micronutrients to the plant. Our findings provide novel insights into the agro‐biofortification of cowpeas and can be further developed to guide the selection of OWPs and MIs for use in agroecological biofortification systems.

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Claims 8

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Application of organic waste products with microbial inocula increased cowpea grain yield by up to 2.4-fold compared with the control.

Organ / stage
grainse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
cowpea yielde1
Direction
increasese1
Value
2.4e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Application of organic waste products with microbial inocula increased cowpea haulm yield by up to 3.2-fold compared with the control.

Organ / stage
haulmse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
cowpea yielde1
Direction
increasese1
Value
3.2e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe concentrations in cowpea grains rose by up to 48% with organic waste products and microbial inocula compared with the control.

Organ / stage
grainse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
Fe concentrationse1
Direction
increaseinferrede1
Value
+48%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fe concentrations in cowpea haulms rose by up to 259% with organic waste products and microbial inocula compared with the control.

Organ / stage
haulmse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
Fe concentrationse1
Direction
increaseinferrede1
Value
+259%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn concentrations in cowpea grains rose by up to 28% with organic waste products and microbial inocula compared with the control.

Organ / stage
grainse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
Zn concentrationse1
Direction
increaseinferrede1
Value
+28%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Zn concentrations in cowpea haulms rose by up to 265% with organic waste products and microbial inocula compared with the control.

Organ / stage
haulmse1
Treatment
application of OWP combined with microbial inoculae1
Outcome
Zn concentrationse1
Direction
increaseinferrede1
Value
+265%e1
Comparator
controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The observed effects on yield and Fe and Zn concentrations depended on the type of organic waste products and microbial inocula used.

Treatment
type of OWPs and MIse2
Outcome
observed effects on the yield and Fe and Zn concentrationse2

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
e2

“In addition, the observed effects on the yield and Fe and Zn concentrations depended on the type of OWPs and MIs used”

✓ located verbatim in the source · abstract, characters 948–1065 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Microbial inocula facilitate the solubilization and transfer of micronutrients to cowpea.

Outcome
solubilization and transfer of these micronutrientse3
Qualifier
MIs that facilitatee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“significant increases in cowpea yield (up to 2.4‐fold for grains and 3.2‐fold for haulms), Fe concentrations (up to +48% in grains and +259% in haulms), and Zn concentrations (up to +28% in grains and +265% in haulms) with the application of OWP combined with microbial inocula (MIs), compared with those in the control”

✓ located verbatim in the source · abstract, characters 627–946 · show in text ↑
e3

“MIs that facilitate the solubilization and transfer of these micronutrients to the plant”

✓ located verbatim in the source · abstract, characters 1269–1357 · show in text ↑

inoculation · yield · Vigna unguiculata (cowpea)Results differ

116 papers: 99 positive, 17 negative or no effect

98 papers in the same direction as this paper · 16 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

112 papers more in this group

See all 116 papers →

inoculation · nutrient: content · Vigna unguiculata (cowpea)Positive and negative results

43 papers: 41 positive, 2 negative or no effect

40 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

39 papers more in this group

See all 43 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.