Paper · 2026 · International Journal of Agronomy

Nutritional Status in Grapevine–Alfalfa Intercropping System on Agricultural and Heavy Metal‐Contaminated Soils

Sabrine Jeder; Issam Nouairi; Fadwa Melki; et al.

12
claims
24
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one. In the present work, we observed that in the agricultural soil, grapevine intercropped with the alfalfa–rhizobium combination exhibited a significant increase in whole plant biomass compared to plants grown in monoculture, reaching values from 26 to 38 g/plant, respectively. However, in the contaminated soil, intercropping had no significant effect on plant biomass (approximately 26 g/plant). In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture. Nevertheless, root Zn, Ca, and Fe concentrations (0.5, 80, and 10 g/kg·DW, respectively) were significantly lower than in grapevines cultivated alone. This suggests that grapevine competes with alfalfa for mineral uptake. In the contaminated soil, a significant reduction in root Fe and Ca concentrations was also observed in intercropped plants compared with monocultured grapevines—approximately 35% and 64% lower, respectively. It is likely that intercropping improves nutrient use efficiency while decreasing trace metal accumulation in intercropped grapevines. In fact, compared with monoculture, intercropping with alfalfa and rhizobia did not enhance mineral nutrient levels in grapevines.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Grapevine intercropped with alfalfa–rhizobium increased whole plant biomass compared with monoculture in agricultural soil.

Taxon
grapevineVitise2
Treatment
alfalfa–rhizobium combinatione1
Interactor
rhizobiumRhizobiume1symbiont
Outcome
whole plant biomasse1
Direction
increasee1
Value
26 to 38 g/plante1
Comparator
monoculturee1
Setting
agricultural soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗
Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“in the agricultural soil, grapevine intercropped with the alfalfa–rhizobium combination exhibited a significant increase in whole plant biomass compared to plants grown in monoculture, reaching values from 26 to 38 g/plant, respectively”

✓ located verbatim in the source · abstract, characters 204–440 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Intercropping had no significant effect on grapevine plant biomass in contaminated soil.

Taxon
grapevineVitise2
Treatment
intercroppinge1
Outcome
plant biomasse1
Direction
no significant effecte1
Value
approximately 26 g/plante1
Setting
contaminated soile1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“However, in the contaminated soil, intercropping had no significant effect on plant biomass (approximately 26 g/plant)”

✓ located verbatim in the source · abstract, characters 442–560 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Shoot Zn, Mg and Ca concentrations of intercropped grapevine did not differ from monoculture in agricultural soil.

Taxon
grapevineVitise2
Organ / stage
shoote1
Treatment
intercroppede1
Outcome
shoot Zn, Mg, Cae1
Direction
no significant differencee1
Comparator
those grown in monoculturee1
Setting
agricultural soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture”

✓ located verbatim in the source · abstract, characters 562–728 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Shoot Fe concentration of intercropped grapevine did not differ from monoculture in agricultural soil.

Taxon
grapevineVitise2
Organ / stage
shoote1
Treatment
intercroppede1
Outcome
Fe concentrationse1
Direction
no significant differencee1
Comparator
those grown in monoculturee1
Setting
agricultural soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture”

✓ located verbatim in the source · abstract, characters 562–728 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Root Zn concentration of intercropped grapevine was significantly lower than in grapevine grown alone in agricultural soil.

Taxon
grapevineVitise3
Organ / stage
roote1
Treatment
intercroppede2
Outcome
root Zn, Ca, and Fe concentrationse1
Direction
lowere1
Value
0.5e1
Comparator
grapevines cultivated alonee1
Setting
agricultural soile2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Nevertheless, root Zn, Ca, and Fe concentrations (0.5, 80, and 10 g/kg·DW, respectively) were significantly lower than in grapevines cultivated alone”

✓ located verbatim in the source · abstract, characters 730–879 · show in text ↑
e2

“In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture”

✓ located verbatim in the source · abstract, characters 562–728 · show in text ↑
e3

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Root Ca concentration of intercropped grapevine was significantly lower than in grapevine grown alone in agricultural soil.

Taxon
grapevineVitise3
Organ / stage
roote1
Treatment
intercroppede2
Outcome
root Zn, Ca, and Fe concentrationse1
Direction
lowere1
Value
80e1
Comparator
grapevines cultivated alonee1
Setting
agricultural soile2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Nevertheless, root Zn, Ca, and Fe concentrations (0.5, 80, and 10 g/kg·DW, respectively) were significantly lower than in grapevines cultivated alone”

✓ located verbatim in the source · abstract, characters 730–879 · show in text ↑
e2

“In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture”

✓ located verbatim in the source · abstract, characters 562–728 · show in text ↑
e3

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Root Fe concentration of intercropped grapevine was significantly lower than in grapevine grown alone in agricultural soil.

Taxon
grapevineVitise3
Organ / stage
roote1
Treatment
intercroppede2
Outcome
root Zn, Ca, and Fe concentrationse1
Direction
lowere1
Value
10 g/kg·DWe1
Comparator
grapevines cultivated alonee1
Setting
agricultural soile2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“Nevertheless, root Zn, Ca, and Fe concentrations (0.5, 80, and 10 g/kg·DW, respectively) were significantly lower than in grapevines cultivated alone”

✓ located verbatim in the source · abstract, characters 730–879 · show in text ↑
e2

“In the agricultural soil, intercropped grapevines showed no significant difference in shoot Zn, Mg, Ca, and Fe concentrations compared with those grown in monoculture”

✓ located verbatim in the source · abstract, characters 562–728 · show in text ↑
e3

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Root Fe concentration of intercropped grapevine was about 35% lower than in monocultured grapevine in contaminated soil.

Taxon
grapevineVitise2
Organ / stage
roote1
Treatment
intercropped plantse1
Outcome
root Fe and Ca concentrationse1
Direction
lowere1
Value
35%e1
Comparator
monocultured grapevinese1
Setting
contaminated soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“In the contaminated soil, a significant reduction in root Fe and Ca concentrations was also observed in intercropped plants compared with monocultured grapevines—approximately 35% and 64% lower, respectively”

✓ located verbatim in the source · abstract, characters 952–1159 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Root Ca concentration of intercropped grapevine was about 64% lower than in monocultured grapevine in contaminated soil.

Taxon
grapevineVitise2
Organ / stage
roote1
Treatment
intercropped plantse1
Outcome
root Fe and Ca concentrationse1
Direction
lowere1
Value
64%e1
Comparator
monocultured grapevinese1
Setting
contaminated soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“In the contaminated soil, a significant reduction in root Fe and Ca concentrations was also observed in intercropped plants compared with monocultured grapevines—approximately 35% and 64% lower, respectively”

✓ located verbatim in the source · abstract, characters 952–1159 · show in text ↑
e2

“Wild grapevine was cultivated either alone or in association with Medicago sativa– rhizobia symbiosis on two soil types: an agricultural soil and a contaminated one”

✓ located verbatim in the source · abstract, characters 0–164 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativebasis n/a

Claim · model-written summary

Grapevine competes with alfalfa for mineral uptake when intercropped.

Taxon
grapevineVitise1
Interactor
alfalfaMedicago sativae1other
Outcome
mineral uptakee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗
Medicago sativa
Medicago sativa · InteractorDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“This suggests that grapevine competes with alfalfa for mineral uptake”

✓ located verbatim in the source · abstract, characters 881–950 · show in text ↑
Associationhypothesisoriginal resultpositivebasis n/a

Claim · model-written summary

Intercropping likely decreases trace metal accumulation in grapevine.

Taxon
grapevineVitise1
Treatment
intercroppinge1
Outcome
trace metal accumulatione1
Direction
decreasinge1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“It is likely that intercropping improves nutrient use efficiency while decreasing trace metal accumulation in intercropped grapevines”

✓ located verbatim in the source · abstract, characters 1161–1294 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Intercropping with alfalfa and rhizobia did not enhance mineral nutrient levels in grapevine compared with monoculture.

Taxon
grapevinesVitise1
Treatment
intercropping with alfalfa and rhizobiae1
Outcome
mineral nutrient levelse1
Direction
did not enhancee1
Comparator
monoculturee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vitis
Vitis · Taxon · picture of the genus (one of its species)Victor M. Vicente Selvas · Public domain · Wikimedia Commons ↗

Verbatim

e1

“In fact, compared with monoculture, intercropping with alfalfa and rhizobia did not enhance mineral nutrient levels in grapevines”

✓ located verbatim in the source · abstract, characters 1296–1425 · show in text ↑

intercropping · growth · VitisPositive and negative results

7 papers: 5 positive, 2 negative or no effect

1 paper in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

intercropping · nutrient: content · VitisPositive and negative results

16 papers: 9 positive, 7 negative or no effect

6 papers in the same direction as this paper · 9 reporting no difference from an alternative

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.