Abstract
A good potential for in vitro regeneration is needed to produce useful plants in genetic improvement programs. Various protocols have been used to improve specifically soybean and cowpea cultivars regeneration. In this study, one cultivar of soybean, “Doko”, and four cowpea cultivars (Epace 10, João Paulo II, Setentão and Pitiuba) served to provide explants for in vitro culture. Zygotic embryos and cotyledons extracted from seeds and segments of hypocotyls taken from germinated seedlings have been cultivated in supplemented environment with different growth regulators (2,4-D, TDZ and BA). MS environment containing BA and 2, 4-D (5 mM) results on a better callus and bud formation on zygotic embryos and cotyledons explants of the cultivar “Doko”. Bud formation from zygotic explants of this cultivar was higher in average in relation to the four cultivars of cowpea cultured in a supplemented environment with BA (6. 6 mM) and sucrose (3%). These in vitro morphogenetic responses don’t allow a further utilization of these materials for genetic improvement.
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Claims 3
Claim · model-written summary
MS medium with BA and 2,4-D improved callus and bud formation in Doko embryos and cotyledons.
- Taxon
- soybean
Glycine maxe1 - Material
- Dokoe0
- Organ / stage
- zygotic embryos and cotyledonse0
- Treatment
- MS environment containing BA and 2, 4-D (5 mM)e0
- Outcome
- callus and bud formatione0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS environment containing BA and 2, 4-D (5 mM) results on a better callus and bud formation on zygotic embryos and cotyledons explants of the cultivar “Doko”.”
e1“soybean and cowpea cultivars”
Claim · model-written summary
Doko had higher bud formation than four cowpea cultivars in BA and sucrose medium.
- Taxon
- soybeanGlycine maxe1
- Material
- Dokoe1
- Outcome
- Bud formatione0
- Direction
- highere0
- Comparator
- four cultivars of cowpeae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bud formation from zygotic explants of this cultivar was higher in average in relation to the four cultivars of cowpea cultured in a supplemented environment with BA (6. 6 mM) and sucrose (3%).”
e1“one cultivar of soybean, “Doko””
Claim · model-written summary
The morphogenetic responses were inadequate for genetic improvement use.
- Taxon
- soybeanGlycine maxe1
- Outcome
- further utilization of these materials for genetic improvemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These in vitro morphogenetic responses don’t allow a further utilization of these materials for genetic improvement.”
e1“soybean and cowpea cultivars”
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