Paper · 2026 · LWT

Effects of iodine, selenium, and zinc biofortification on wheat flour and dough properties for bread making

Hind Belarbi; Fassil Kebede; Ingrid De Leyn; et al.

17
claims
27
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by-nc

Abstract

Micronutrient deficiencies, or hidden hunger, remain a critical global nutritional challenge. Agronomic biofortification of staple crops like wheat with iodine (I), selenium (Se), and zinc (Zn) represents a promising solution to address this issue. This study assessed the impact of foliar-applied I, Se, and Zn, individually and in combination, on flour and bread quality in two wheat cultivars. In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively. Co-application of nutrients reduced protein content by ∼10% and slightly decreased dough gas retention (77% to 75%) yet had minimal effect on bread volume. Individually, Zn biofortification reduced loaf volume, whereas Se and I treatments maintained it. Moreover, pan loaf bread preserved more micronutrient concentrations than flatbread. Despite variations in micronutrient retention among bread types, biofortified wheat presents a sustainable approach to enhancing dietary micronutrient intake without compromising bread-making quality. • Foliar I, Se, and Zn application increased micronutrients in grain, flour, and bread. • White flour retained 32% I, 74% Se, and 23% Zn of whole grain concentrations. • Pan loaf baking preserved more I, Se, and Zn than flatbread baking. • Bread volume was unaffected, except under Zn treatment, which reduced loaf size. • A 100 g serving of biofortified bread provides up to 43% I, 56% Se, and 41% Zn RDA.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 17

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar application of I, Se and Zn increased micronutrients in wheat grain, flour and bread.

Organ / stage
grain, flour, and breade1
Treatment
Foliar I, Se, and Zn applicatione1
Outcome
micronutrientse1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e1

“Foliar I, Se, and Zn application increased micronutrients in grain, flour, and bread”

✓ located verbatim in the source · abstract, characters 1098–1182 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg iodine.

Material
Bezostaja-1e0
Organ / stage
whole wheat floure0
Outcome
Ie0
Value
619 μg/kge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In Bezostaja-1, biofortified whole wheat flour reached 623 μg/kg selenium.

Material
Bezostaja-1e0
Organ / stage
whole wheat floure0
Outcome
See0
Value
623 μg/kge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In Bezostaja-1, biofortified whole wheat flour reached 75 mg/kg zinc.

Material
Bezostaja-1e0
Organ / stage
whole wheat floure0
Outcome
Zne0
Value
75 mg/kge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

White flour retained 32% of the whole wheat flour iodine.

Material
Bezostaja-1e0
Organ / stage
white floure0
Outcome
Ie0
Value
32%e0
Comparator
biofortified whole wheat floure0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

White flour retained 74% of the whole wheat flour selenium.

Material
Bezostaja-1e0
Organ / stage
white floure0
Outcome
See0
Value
74%e0
Comparator
biofortified whole wheat floure0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

White flour retained 23% of the whole wheat flour zinc.

Material
Bezostaja-1e0
Organ / stage
white floure0
Outcome
Zne0
Value
23%e0
Comparator
biofortified whole wheat floure0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In Bezostaja-1, biofortified whole wheat flour reached 619 μg/kg I, 623 μg/kg Se, and 75 mg/kg Zn, with white flour retaining 32%, 74%, and 23%, respectively”

✓ located verbatim in the source · abstract, characters 397–554 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Co-application of the nutrients reduced wheat protein content by about 10%.

Treatment
Co-application of nutrientse2
Outcome
protein contente2
Direction
reducede2
Value
∼10%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e2

“Co-application of nutrients reduced protein content by ∼10% and slightly decreased dough gas retention (77% to 75%) yet had minimal effect on bread volume”

✓ located verbatim in the source · abstract, characters 556–710 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Co-application of the nutrients slightly decreased dough gas retention, from 77% to 75%.

Treatment
Co-application of nutrientse2
Outcome
dough gas retentione2
Direction
decreasede2
Value
77% to 75%e2
Qualifier
slightlye2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e2

“Co-application of nutrients reduced protein content by ∼10% and slightly decreased dough gas retention (77% to 75%) yet had minimal effect on bread volume”

✓ located verbatim in the source · abstract, characters 556–710 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Co-application of the nutrients had a minimal effect on wheat bread volume.

Treatment
Co-application of nutrientse2
Outcome
bread volumee2
Direction
no_changeinferrede2
Qualifier
minimal effecte2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e2

“Co-application of nutrients reduced protein content by ∼10% and slightly decreased dough gas retention (77% to 75%) yet had minimal effect on bread volume”

✓ located verbatim in the source · abstract, characters 556–710 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Zn biofortification reduced wheat loaf volume.

Treatment
Zn biofortificatione3
Outcome
loaf volumee3
Direction
reducede3
Qualifier
Individuallye3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e3

“Individually, Zn biofortification reduced loaf volume, whereas Se and I treatments maintained it”

✓ located verbatim in the source · abstract, characters 712–808 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Se and I biofortification maintained wheat loaf volume.

Treatment
Se and I treatmentse3
Outcome
loaf volumee3
Direction
maintainede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e3

“Individually, Zn biofortification reduced loaf volume, whereas Se and I treatments maintained it”

✓ located verbatim in the source · abstract, characters 712–808 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pan loaf bread preserved more wheat micronutrient concentrations than flatbread.

Treatment
pan loaf breade4
Outcome
micronutrient concentrationse4
Direction
preserved moree4
Comparator
flatbreade4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e4

“Moreover, pan loaf bread preserved more micronutrient concentrations than flatbread”

✓ located verbatim in the source · abstract, characters 810–893 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A 100 g serving of biofortified wheat bread provides up to 43% of the RDA for iodine.

Outcome
Ie5
Value
43%e5
Qualifier
RDAe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e5

“A 100 g serving of biofortified bread provides up to 43% I, 56% Se, and 41% Zn RDA”

✓ located verbatim in the source · abstract, characters 1419–1501 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A 100 g serving of biofortified wheat bread provides up to 56% of the RDA for selenium.

Outcome
See5
Value
56%e5
Qualifier
RDAe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e5

“A 100 g serving of biofortified bread provides up to 43% I, 56% Se, and 41% Zn RDA”

✓ located verbatim in the source · abstract, characters 1419–1501 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A 100 g serving of biofortified wheat bread provides up to 41% of the RDA for zinc.

Outcome
Zne5
Value
41%e5
Qualifier
RDAe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“on flour and bread quality in two wheat cultivars”

✓ located verbatim in the source · abstract, characters 346–395 · show in text ↑
e5

“A 100 g serving of biofortified bread provides up to 43% I, 56% Se, and 41% Zn RDA”

✓ located verbatim in the source · abstract, characters 1419–1501 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Biofortified wheat is a sustainable approach to enhancing dietary micronutrient intake without compromising bread-making quality, according to the authors.

Outcome
dietary micronutrient intakee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“biofortified wheat presents a sustainable approach to enhancing dietary micronutrient intake without compromising bread-making quality”

✓ located verbatim in the source · abstract, characters 960–1094 · show in text ↑

zinc atom · molecular: metabolites · Triticum aestivum (wheat)Results differ

111 papers: 85 positive, 26 negative or no effect

84 papers in the same direction as this paper · 25 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

107 papers more in this group

See all 111 papers →

selenium atom · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

27 papers: 22 positive, 5 negative or no effect

21 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. negative
  3. no effect

23 papers more in this group

See all 27 papers →

application · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

115 papers: 97 positive, 18 negative or no effect

96 papers in the same direction as this paper · 18 in the opposite direction

  1. positive
  2. positive
  3. negative

111 papers more in this group

See all 115 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.