Paper · 2025 · Current Agronomy

Effects of the foliar application of humic biostimulant on alfalfa and cucumber – case studies

Jacek Sosnowski; Robert Rosa; Krzysztof Pakuła; et al.

9
claims
13
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Abstract The aim of the research was to evaluate the effect of the new humic biostimulants preparation containing fulvic acids on the growth and quality of the yield of alfalfa ( Medicago × varia T. Martyn) and cucumber ( Cucumis sativus L.). In 2025, two experiments were conducted: 1. with alfalfa – a grow room experiment, and 2. with cucumber – a field experiment. Experimental factors: I – biostimulant, II – number of applications (2 and 4 sprays). The concentration of the preparation and the number and timing of treatments are recommended by the manufacturer. Morphometric characteristics (alfalfa), marketable yield (cucumber) and chemical composition (ICP-AES method) were determined in the plant material. Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot. It also resulted in an increase of up to 61% in the dry weight of plants compared to the control. Moreover, it increased the concentration of potassium, magnesium, and phosphorus in the dry matter of the plant. Foliar application of the tested biostimulant to cucumbers resulted in a statistically significant increase in fruit yield of gherkin cucumbers by 24% and in total marketable yield by 12.5% compared to the control. No significant changes were observed in the dry matter, protein, phosphorus, potassium, or magnesium content in cucumber fruit. The research conducted is a preliminary step towards expanding the assessment of the effects of fulvic acid use on the characteristics of tested plants under various growing conditions.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The tested biostimulant increased the length of stalks, number of shoots per plant and leaves per shoot in alfalfa.

Taxon
alfalfaMedicago sativae1
Treatment
Application of tested biostimulante1
Outcome
the length of stalks, number of shoots per plant and leaves per shoote1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot”

✓ located verbatim in the source · abstract, characters 718–991 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The tested biostimulant increased root collar diameter and shoot and root system weight per plant in alfalfa.

Taxon
alfalfaMedicago sativae1
Treatment
Application of tested biostimulante1
Outcome
diameter of root collar, weight of shoot and root system per plante1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot”

✓ located verbatim in the source · abstract, characters 718–991 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The tested biostimulant increased leaf and inflorescence weight per shoot in alfalfa.

Taxon
alfalfaMedicago sativae1
Treatment
Application of tested biostimulante1
Outcome
weight of leaves and inflorescence per shoote1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot”

✓ located verbatim in the source · abstract, characters 718–991 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The tested biostimulant increased alfalfa dry weight by up to 61% compared with the control.

Taxon
alfalfaMedicago sativae1
Treatment
tested biostimulante1
Outcome
dry weight of plantse2
Direction
increasee2
Value
up to 61%e2
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot”

✓ located verbatim in the source · abstract, characters 718–991 · show in text ↑
e2

“It also resulted in an increase of up to 61% in the dry weight of plants compared to the control”

✓ located verbatim in the source · abstract, characters 993–1089 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The tested biostimulant increased potassium, magnesium and phosphorus concentration in alfalfa dry matter.

Taxon
alfalfaMedicago sativae1
Treatment
tested biostimulante1
Outcome
concentration of potassium, magnesium, and phosphoruse3
Direction
increasede3
Qualifier
in the dry matter of the plante3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Application of tested biostimulant increased the value of morphometric traits of alfalfa: the length of stalks, number of shoots per plant and leaves per shoot, diameter of root collar, weight of shoot and root system per plant, weight of leaves and inflorescence per shoot”

✓ located verbatim in the source · abstract, characters 718–991 · show in text ↑
e3

“Moreover, it increased the concentration of potassium, magnesium, and phosphorus in the dry matter of the plant”

✓ located verbatim in the source · abstract, characters 1091–1202 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar application of the tested biostimulant increased gherkin cucumber fruit yield by 24% compared with the control.

Taxon
cucumbersCucumis sativuse4
Material
gherkin cucumberse4
Treatment
Foliar application of the tested biostimulante4
Outcome
fruit yielde4
Direction
increasee4
Value
24%e4
Comparator
controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“Foliar application of the tested biostimulant to cucumbers resulted in a statistically significant increase in fruit yield of gherkin cucumbers by 24% and in total marketable yield by 12.5% compared to the control”

✓ located verbatim in the source · abstract, characters 1204–1417 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar application of the tested biostimulant increased total marketable cucumber yield by 12.5% compared with the control.

Taxon
cucumbersCucumis sativuse4
Treatment
Foliar application of the tested biostimulante4
Outcome
total marketable yielde4
Direction
increasee4
Value
12.5%e4
Comparator
controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“Foliar application of the tested biostimulant to cucumbers resulted in a statistically significant increase in fruit yield of gherkin cucumbers by 24% and in total marketable yield by 12.5% compared to the control”

✓ located verbatim in the source · abstract, characters 1204–1417 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The tested biostimulant did not change dry matter, protein or phosphorus content in cucumber fruit.

Taxon
cucumbersCucumis sativuse4
Organ / stage
cucumber fruite5
Treatment
Foliar application of the tested biostimulante4
Outcome
dry matter, protein, phosphoruse5
Direction
no_changeinferrede5
Comparator
controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“Foliar application of the tested biostimulant to cucumbers resulted in a statistically significant increase in fruit yield of gherkin cucumbers by 24% and in total marketable yield by 12.5% compared to the control”

✓ located verbatim in the source · abstract, characters 1204–1417 · show in text ↑
e5

“No significant changes were observed in the dry matter, protein, phosphorus, potassium, or magnesium content in cucumber fruit”

✓ located verbatim in the source · abstract, characters 1419–1545 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The tested biostimulant did not change potassium or magnesium content in cucumber fruit.

Taxon
cucumbersCucumis sativuse4
Organ / stage
cucumber fruite5
Treatment
Foliar application of the tested biostimulante4
Outcome
potassium, or magnesiume5
Direction
no_changeinferrede5
Comparator
controle4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“Foliar application of the tested biostimulant to cucumbers resulted in a statistically significant increase in fruit yield of gherkin cucumbers by 24% and in total marketable yield by 12.5% compared to the control”

✓ located verbatim in the source · abstract, characters 1204–1417 · show in text ↑
e5

“No significant changes were observed in the dry matter, protein, phosphorus, potassium, or magnesium content in cucumber fruit”

✓ located verbatim in the source · abstract, characters 1419–1545 · show in text ↑

application · quality: protein · Cucumis sativus (cucumber)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

other · nutrient: content · Cucumis sativus (cucumber)Positive and negative results

9 papers: 7 positive, 2 negative or no effect

1 paper in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

application · quality: postharvest · Cucumis sativus (cucumber)Positive and negative results

10 papers: 8 positive, 2 negative or no effect

7 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

6 papers more in this group

See all 10 papers →

application · nutrient: content · Cucumis sativus (cucumber)Positive and negative results

20 papers: 15 positive, 5 negative or no effect

4 papers in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

other · yield · Cucumis sativus (cucumber)Positive and negative results

23 papers: 21 positive, 2 negative or no effect

20 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. negative
  3. no effect

19 papers more in this group

See all 23 papers →

application · yield · Cucumis sativus (cucumber)Positive and negative results

73 papers: 65 positive, 8 negative or no effect

64 papers in the same direction as this paper · 7 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

69 papers more in this group

See all 73 papers →

other · molecular: metabolites · Medicago sativa (alfalfa)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

other · quality: postharvest · Cucumis sativus (cucumber)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.