Abstract
The plant endomembrane system and vesicle trafficking are central to plant immunity, mediating the targeted delivery and recycling of defence molecules during pathogen attack. Here, we investigated the functional role of soybean Vacuole Membrane Protein 1 (GmVMP1) in mediating resistance against soybean cyst nematode (SCN, Heterodera glycines). GmVMP1 is a SNARE-associated protein whose expression was specifically and transiently upregulated in SCN-resistant soybean roots. Overexpression of GmVMP1 conferred near-complete resistance to SCN. Field-grown transgenic lines showed no developmental penalties and displayed modest gains in seed weight and protein content. GmVMP1 interacted with four key vesicle trafficking proteins, and silencing these interactors compromised GmVMP1-mediated resistance. Live-cell imaging revealed that GmVMP1 enhances endocytic vesicle formation and accelerates internalisation dynamics, pointing to a role in membrane trafficking during defence activation. Together, these results establish GmVMP1 as a novel SCN resistance gene that modulates vesicle trafficking to support early defence, with promising agronomic traits for soybean improvement.
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Claims 8
Claim · model-written summary
GmVMP1 expression rose transiently in SCN-resistant soybean roots.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- rootse0
- Interactor
- SCNe0pathogen
- Gene
- GmVMP1e0
- Outcome
- expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmVMP1 is a SNARE-associated protein whose expression was specifically and transiently upregulated in SCN-resistant soybean roots.”
Claim · model-written summary
Overexpressing GmVMP1 gave near-complete resistance to SCN.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Overexpression of GmVMP1e0
- Interactor
- SCNe0pathogen
- Gene
- GmVMP1e0
- Outcome
- resistance to SCNe0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GmVMP1 conferred near-complete resistance to SCN.”
e1“soybean roots”
Claim · model-written summary
Field-grown transgenic lines showed no developmental penalties.
- Taxon
- soybeanGlycine maxe1
- Material
- transgenic linese0
- Outcome
- developmental penaltiese0
- Direction
- noe0
- Setting
- Field-growne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field-grown transgenic lines showed no developmental penalties”
e1“soybean roots”
Claim · model-written summary
Field-grown transgenic lines had modest gains in seed weight and protein content.
- Taxon
- soybeanGlycine maxe1
- Material
- transgenic linese0
- Outcome
- seed weight and protein contente0
- Direction
- gainse0
- Setting
- Field-growne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field-grown transgenic lines showed no developmental penalties and displayed modest gains in seed weight and protein content.”
e1“soybean roots”
Claim · model-written summary
GmVMP1 interacted with four vesicle trafficking proteins.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmVMP1e0
- Outcome
- interacted with four key vesicle trafficking proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmVMP1 interacted with four key vesicle trafficking proteins”
e1“soybean roots”
Claim · model-written summary
Silencing the GmVMP1 interactors compromised GmVMP1-mediated resistance.
- Taxon
- soybeanGlycine maxe1
- Treatment
- silencing these interactorse0
- Interactor
- SCNe2pathogen
- Gene
- GmVMP1e0
- Outcome
- GmVMP1-mediated resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing these interactors compromised GmVMP1-mediated resistance.”
e1“soybean roots”
e2“soybean cyst nematode (SCN, Heterodera glycines)”
Claim · model-written summary
GmVMP1 enhanced endocytic vesicle formation.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmVMP1e0
- Outcome
- endocytic vesicle formatione0
- Direction
- enhancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmVMP1 enhances endocytic vesicle formation and accelerates internalisation dynamics”
e1“soybean roots”
Claim · model-written summary
GmVMP1 accelerated internalisation dynamics.
- Taxon
- soybeanGlycine maxe1
- Gene
- GmVMP1e0
- Outcome
- internalisation dynamicse0
- Direction
- acceleratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmVMP1 enhances endocytic vesicle formation and accelerates internalisation dynamics”
e1“soybean roots”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
19 papers in the same direction as this paper · 17 in the opposite direction
- negative
- positive
- positive
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.