Abstract
Wheat leaf rust, caused by Puccinia triticina, poses a significant threat to global wheat production. MicroRNAs (miRNAs) are critical regulators of plant growth, development and stress responses; however, their role in wheat resistance to leaf rust remains poorly understood. In this study, we identified the miR171a-TaSCL6-1 module as a key regulator of leaf rust resistance in wheat. Expression of miR171a was upregulated in wheat seedlings following pathogen inoculation. Transgenic assays, including overexpression and short tandem target mimic approaches, demonstrated that miR171a negatively regulates resistance to leaf rust. Degradome sequencing confirmed TaSCL6-1 as the primary target of miR171a, and both overexpression and knockout of TaSCL6-1 established its role as a positive regulator of resistance. Transcriptome analysis revealed that TaSCL6-1 upregulates defence-related genes encoding peroxidases and transcription factors. TaSCL6-1 can activate the transcription of downstream genes by binding to the GAA motif in the promoters, and silencing of downstream genes (TaPOD2, TaPOD35 and TaERF114) increased wheat susceptibility to leaf rust. Furthermore, miR164-targeted TaNAC21/22 acts as an upstream regulator of MIR171a by binding to its promoter and activating its expression. Overexpression of TaNAC21/22 increased susceptibility to leaf rust, whereas transient silencing of TaNAC21/22 or overexpression of miR164 enhanced seedling resistance to leaf rust. Our findings elucidate the role of the miR171a-TaSCL6-1 module in regulating wheat defence against leaf rust, providing potential genetic targets for breeding resistant wheat varieties.
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Claims 11
Claim · model-written summary
Pathogen inoculation upregulated miR171a expression in wheat seedlings.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- seedlingse0
- Treatment
- pathogen inoculatione0
- Interactor
- Puccinia triticina
Puccinia triticinae1pathogen - Gene
- miR171ae0
- Outcome
- Expression of miR171ae0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of miR171a was upregulated in wheat seedlings following pathogen inoculation.”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
miR171a negatively regulates wheat leaf rust resistance.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- miR171ae0
- Outcome
- resistance to leaf ruste0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR171a negatively regulates resistance to leaf rust”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
TaSCL6-1 is the primary target of miR171a.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaSCL6-1e0
- Outcome
- primary target of miR171ae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Degradome sequencing confirmed TaSCL6-1 as the primary target of miR171a”
t0“Wheat”
Claim · model-written summary
TaSCL6-1 positively regulates wheat leaf rust resistance.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- TaSCL6-1e0
- Outcome
- resistancee0
- Direction
- positive regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both overexpression and knockout of TaSCL6-1 established its role as a positive regulator of resistance”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
TaSCL6-1 upregulates defense genes encoding peroxidases and transcription factors.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaSCL6-1e0
- Outcome
- defence-related genes encoding peroxidases and transcription factorse0
- Direction
- upregulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSCL6-1 upregulates defence-related genes encoding peroxidases and transcription factors”
t0“Wheat”
Claim · model-written summary
TaSCL6-1 activates downstream gene transcription by binding the GAA promoter motif.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaSCL6-1e0
- Outcome
- transcription of downstream genese0
- Direction
- activatee0
- Qualifier
- binding to the GAA motif in the promoterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSCL6-1 can activate the transcription of downstream genes by binding to the GAA motif in the promoters”
t0“Wheat”
Claim · model-written summary
Silencing TaPOD2, TaPOD35 and TaERF114 increased wheat leaf rust susceptibility.
- Taxon
- wheatTriticum aestivume0
- Treatment
- silencing of downstream genese0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- TaPOD2, TaPOD35 and TaERF114e0
- Outcome
- wheat susceptibility to leaf ruste0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of downstream genes (TaPOD2, TaPOD35 and TaERF114) increased wheat susceptibility to leaf rust”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
TaNAC21/22 binds the MIR171a promoter and activates its expression.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNAC21/22e0
- Outcome
- expressione0
- Direction
- activatinge0
- Qualifier
- binding to its promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR164-targeted TaNAC21/22 acts as an upstream regulator of MIR171a by binding to its promoter and activating its expression”
t0“Wheat”
Claim · model-written summary
Overexpressing TaNAC21/22 increased wheat leaf rust susceptibility.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of TaNAC21/22e0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- TaNAC21/22e0
- Outcome
- susceptibility to leaf ruste0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of TaNAC21/22 increased susceptibility to leaf rust”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
Transient silencing of TaNAC21/22 enhanced wheat seedling resistance to leaf rust.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- transient silencing of TaNAC21/22e0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- TaNAC21/22e0
- Outcome
- seedling resistance to leaf ruste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing of TaNAC21/22 or overexpression of miR164 enhanced seedling resistance to leaf rust”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
Claim · model-written summary
Overexpression of miR164 enhanced wheat seedling resistance to leaf rust.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of miR164e0
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- miR164e0
- Outcome
- seedling resistance to leaf ruste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of miR164 enhanced seedling resistance to leaf rust”
t0“Wheat”
e1“Wheat leaf rust, caused by Puccinia triticina”
The same questions in other papers
inoculation · Puccinia triticina · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
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See all 6 papers →loss of function · Puccinia triticina · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.