Paper · 2026 · Grassland Science

Calcium deficiency symptoms in forage corn ( Zea mays L.) due to excessive potassium supply under field conditions: Using leaf serration as a soil potassium indicator

Yoshihito Sunaga; Hisatomi Harada; Tahei Kawachi

12
claims
12
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

Abstract Leaf serration in forage corn ( Zea mays L.) is often observed under field conditions. In this study, field cultivation experiments were conducted over 3 years to determine the factors affecting the appearance and development of this trait and to assess its potential impact on corn productivity. We also evaluated soil mineral conditions using leaf serration as an indicator. The number of symptomatic leaves per plant decreased after spraying with calcium chloride solution (3 g/L), and the development of leaf serration was also suppressed. Therefore, leaf serration in corn grown under field conditions may be a symptom of calcium deficiency. Occurrence of leaf serration did not significantly affect the dry matter yield of corn shoots at the yellow‐ripe stage, which is considered optimal for ensiling. This was because the yield, which included the leaves, stems, and ears, was not affected by calcium spraying. The number of leaves with symptoms increased logarithmically with an increase in soil exchangeable potassium content, owing to the decrease in calcium concentration in the corn plant caused by excessive amounts of soil potassium. The appearance of symptoms differed significantly among corn varieties, and the highest number of symptomatic leaves occurred in the variety with the highest calcium concentration. Different regression equations were obtained from the relationships between soil exchangeable potassium content and the number of symptomatic leaves in varieties with low and high calcium concentrations. The soil exchangeable potassium content was estimated using these equations, with the number of symptomatic corn leaves after silking as an indicator. With two or more symptomatic leaves per plant, the soil exchangeable potassium content was adequate for forage corn production. We suggest that a reduction in potassium input should be implemented in fertilization management if corn plants develop leaf serration.

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Claims 12

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Calcium chloride spraying reduced the number of symptomatic leaves per corn plant.

Treatment
spraying with calcium chloride solution (3 g/L)e0
Outcome
number of symptomatic leaves per plante0
Direction
decreasede0

Not reportedTaxonComparator

Verbatim

e0

“The number of symptomatic leaves per plant decreased after spraying with calcium chloride solution (3 g/L)”

✓ located verbatim in the source · abstract, characters 386–492 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Calcium chloride spraying suppressed development of leaf serration.

Outcome
development of leaf serratione0
Direction
suppressede0

Not reportedTaxonTreatmentComparator

Verbatim

e0

“and the development of leaf serration was also suppressed”

✓ located verbatim in the source · abstract, characters 494–551 · show in text ↑
Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Leaf serration did not significantly affect corn shoot dry matter yield at the yellow-ripe stage.

Organ / stage
shoots at the yellow‐ripe stagee0
Outcome
dry matter yielde0
Direction
did not significantly affecte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Occurrence of leaf serration did not significantly affect the dry matter yield of corn shoots at the yellow‐ripe stage”

✓ located verbatim in the source · abstract, characters 656–774 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Calcium spraying did not affect corn yield.

Treatment
calcium sprayinge0
Outcome
yielde0
Direction
not affectede0

Not reportedTaxonComparator

Verbatim

e0

“the yield, which included the leaves, stems, and ears, was not affected by calcium spraying”

✓ located verbatim in the source · abstract, characters 835–926 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Symptomatic leaf number increased logarithmically with soil exchangeable potassium content.

Outcome
number of leaves with symptomse0
Direction
increased logarithmicallye0
Setting
soile0

Not reportedTaxon

Verbatim

e0

“The number of leaves with symptoms increased logarithmically with an increase in soil exchangeable potassium content”

✓ located verbatim in the source · abstract, characters 928–1044 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Excessive soil potassium lowered corn plant calcium concentration.

Treatment
excessive amounts of soil potassiume0
Outcome
calcium concentratione0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“decrease in calcium concentration in the corn plant caused by excessive amounts of soil potassium”

✓ located verbatim in the source · abstract, characters 1059–1156 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Corn varieties differed significantly in symptom appearance.

Material
corn varietiese0
Outcome
appearance of symptomse0
Direction
differed significantlye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The appearance of symptoms differed significantly among corn varieties”

✓ located verbatim in the source · abstract, characters 1158–1228 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The variety with the highest calcium concentration had the most symptomatic leaves.

Material
the varietye0
Outcome
number of symptomatic leavese0
Direction
higheste0

Not reportedTaxon

Verbatim

e0

“the highest number of symptomatic leaves occurred in the variety with the highest calcium concentration”

✓ located verbatim in the source · abstract, characters 1234–1337 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Varieties with low and high calcium concentrations had different regression equations relating soil potassium to symptomatic leaf number.

Outcome
regression equationse0
Comparator
varieties with low and high calcium concentrationse0

Not reportedTaxon

Verbatim

e0

“Different regression equations were obtained from the relationships between soil exchangeable potassium content and the number of symptomatic leaves in varieties with low and high calcium concentrations”

✓ located verbatim in the source · abstract, characters 1339–1541 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The equations estimated soil exchangeable potassium from symptomatic leaf number after silking.

Outcome
soil exchangeable potassium contente0
Comparator
number of symptomatic corn leaves after silkinge0

Not reportedValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The soil exchangeable potassium content was estimated using these equations, with the number of symptomatic corn leaves after silking as an indicator”

✓ located verbatim in the source · abstract, characters 1543–1692 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Two or more symptomatic leaves per plant indicated adequate soil exchangeable potassium for forage corn production.

Taxon
forage corne0
Outcome
soil exchangeable potassium contente0
Direction
adequatee0
Value
two or more symptomatic leaves per plante0

Verbatim

e0

“With two or more symptomatic leaves per plant, the soil exchangeable potassium content was adequate for forage corn production”

✓ located verbatim in the source · abstract, characters 1694–1820 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend reducing potassium input when corn develops leaf serration.

Treatment
fertilization managemente0
Outcome
reduction in potassium inpute0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“We suggest that a reduction in potassium input should be implemented in fertilization management if corn plants develop leaf serration”

✓ located verbatim in the source · abstract, characters 1822–1956 · show in text ↑

calcium atom · yield · Zea mays (maize)Results differ

19 papers: 14 positive, 5 negative or no effect

4 papers in the same direction as this paper · 14 in the opposite direction

  1. positive
  2. positive
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calcium chloride dihydrate · growth · Zea mays (maize)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

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  2. positive
  3. positive

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application · growth · Zea mays (maize)Positive and negative results

337 papers: 310 positive, 27 negative or no effect

309 papers in the same direction as this paper · 25 in the opposite direction · 2 reporting no difference from an alternative

  1. no effect
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application · yield · Zea mays (maize)Positive and negative results

629 papers: 575 positive, 54 negative or no effect

53 papers in the same direction as this paper · 575 in the opposite direction

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.