Abstract
Abstract Camellia sinensis (L.) O. Kuntze exhibits severely restricted growth at low temperatures, resulting in reduced tea leaf yield and quality. BRI1-EMS-suppressor (BES) transcription factors, as key components of the brassinosteroids (BR) signaling pathway, are highly homologous to BZR and jointly regulate plants’ adaptation to environmental stress. In this study, the CsBES1-14 gene was successfully cloned and identified from the transcriptome database of tea plant. Biochemical analyses identified CsBES1-14 as a nuclear localized transcriptional activator, and BR and low temperature induced its expression. Arabidopsis thaliana plants overexpressing CsBES1-14 exhibited increased chilling tolerance by promoting root growth and increasing the expression of cold responsive genes. Conversely, the suppression of CsBES1-14 through virus-induced gene silencing (VIGS) in tea plant notably impaired cold tolerance. Transcription Factor-centered Yeast One-Hybrid screening identified CsCOR413 as a downstream target, and electrophoretic mobility shift assays confirmed the direct binding of CsBES1-14 to specific cis-elements in the CsCOR413 promoter. Exogenous application of brassinazole (BRZ) and VIGS silencing experiments verified that the ICE-CBF cold response pathway could regulate the low-temperature-regulated protein CsCOR413. In summary, these findings elucidate that CsCOR413 expression is modulated not only by the classic ICE-CBF signaling pathway but also directly regulated by CsBES1-14. These findings outline the key components of the cold resistance network in tea plant and provide novel molecular targets for genetic improvement strategies in perennial crops.
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Claims 11
Claim · model-written summary
The CsBES1-14 gene was cloned and identified from the tea plant transcriptome database.
Verbatim
e0“the CsBES1-14 gene was successfully cloned and identified from the transcriptome database of tea plant”
Claim · model-written summary
Biochemical analyses identified CsBES1-14 as a nuclear-localized transcriptional activator.
Verbatim
t0“cold resistance in tea plant”
e0“Biochemical analyses identified CsBES1-14 as a nuclear localized transcriptional activator”
Claim · model-written summary
Brassinosteroid induced CsBES1-14 expression.
Not reportedComparator
Verbatim
t0“cold resistance in tea plant”
e0“Biochemical analyses identified CsBES1-14 as a nuclear localized transcriptional activator, and BR and low temperature induced its expression”
Claim · model-written summary
Low temperature induced CsBES1-14 expression.
Not reportedComparator
Verbatim
t0“cold resistance in tea plant”
e0“Biochemical analyses identified CsBES1-14 as a nuclear localized transcriptional activator, and BR and low temperature induced its expression”
Claim · model-written summary
Arabidopsis thaliana plants overexpressing CsBES1-14 showed increased chilling tolerance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- overexpressing CsBES1-14e0
- Gene
- CsBES1-14e0
- Outcome
- chilling tolerancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana plants overexpressing CsBES1-14 exhibited increased chilling tolerance by promoting root growth and increasing the expression of cold responsive genes”
Claim · model-written summary
Arabidopsis thaliana plants overexpressing CsBES1-14 showed promoted root growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- overexpressing CsBES1-14e0
- Gene
- CsBES1-14e0
- Outcome
- root growthe0
- Direction
- promotinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana plants overexpressing CsBES1-14 exhibited increased chilling tolerance by promoting root growth and increasing the expression of cold responsive genes”
Claim · model-written summary
Arabidopsis thaliana plants overexpressing CsBES1-14 showed increased expression of cold responsive genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- overexpressing CsBES1-14e0
- Gene
- CsBES1-14e0
- Outcome
- expression of cold responsive genese0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana plants overexpressing CsBES1-14 exhibited increased chilling tolerance by promoting root growth and increasing the expression of cold responsive genes”
Claim · model-written summary
Silencing CsBES1-14 by VIGS in tea plant notably impaired cold tolerance.
- Taxon
- tea plante0
- Treatment
- suppression of CsBES1-14 through virus-induced gene silencing (VIGS)e0
- Gene
- CsBES1-14e0
- Outcome
- cold tolerancee0
- Direction
- impairede0
Not reportedComparator
Verbatim
t0“cold resistance in tea plant”
e0“the suppression of CsBES1-14 through virus-induced gene silencing (VIGS) in tea plant notably impaired cold tolerance”
Claim · model-written summary
Yeast one-hybrid screening identified CsCOR413 as a downstream target of CsBES1-14.
Verbatim
t0“cold resistance in tea plant”
e0“Transcription Factor-centered Yeast One-Hybrid screening identified CsCOR413 as a downstream target”
Claim · model-written summary
Electrophoretic mobility shift assays confirmed direct binding of CsBES1-14 to cis-elements in the CsCOR413 promoter.
Verbatim
t0“cold resistance in tea plant”
e0“electrophoretic mobility shift assays confirmed the direct binding of CsBES1-14 to specific cis-elements in the CsCOR413 promoter”
Claim · model-written summary
Brassinazole application and VIGS experiments verified that the ICE-CBF cold response pathway can regulate CsCOR413.
Verbatim
t0“cold resistance in tea plant”
e0“Exogenous application of brassinazole (BRZ) and VIGS silencing experiments verified that the ICE-CBF cold response pathway could regulate the low-temperature-regulated protein CsCOR413”
The same questions in other papers
loss of function · tolerance: abiotic · Camellia sinensis (black tea)Positive and negative results
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