Abstract
The responses of some enzymes, Which are concerned in glucose catabolism, to light were examined, to elucidate the mechanism of the promoting effect of light on the growth of the green tobacco callus tissue. The four kinds of enzymes were examined in this experiment ; glucose-6-phosphte dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), alcohol dehydrogenase (ADH) and malate dehydrogenase (MDH). Among them, the activity of G6PD was observed to be most promoted by light. The increase of G6PD activity occurred only in the callus cells cultured under a strong light and its increase was repressed when DCMU, an inhibitor of oxygen evolution in photosynthesis, was added to the medium. When the callus cells were illuminated again after darkincubation, G6PD activity increased rapidly even in the cells cultured for 7 days in the dark. The degree of the increase, however, reduced in proportion to the length of the dark-incubation period. These results doubtlessly suggest that there is some acceptor of light in chloroplasts, which induces the increase of G6PD. On the other hand, G6PD activity in white tobacco callus (XD-6) tissue, which can grow actively even in the dark and whose growth is not promoted by light, was not enhanced by light. Enhancement of G6PD activity by light is specific for the green callus enzyme and it is inferred that it contributes to the photomixotrophic growth of the green tobacco callus tissue.
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Claims 11
Claim · model-written summary
Light promoted G6PD activity most among the tested enzymes.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- lighte0
- Gene
- G6PDe0
- Outcome
- activity of G6PDe0
- Direction
- most promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among them, the activity of G6PD was observed to be most promoted by light.”
t0“Tobacco”
Claim · model-written summary
G6PD activity increased only under strong light.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- callus cellse0
- Treatment
- strong lighte0
- Gene
- G6PDe0
- Outcome
- G6PD activitye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase of G6PD activity occurred only in the callus cells cultured under a strong light”
t0“Tobacco”
Claim · model-written summary
DCMU repressed the light-induced G6PD activity increase.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its increase was repressed when DCMU, an inhibitor of oxygen evolution in photosynthesis, was added to the medium”
t0“Tobacco”
e2“Among them, the activity of G6PD was observed to be most promoted by light.”
Claim · model-written summary
Reillumination rapidly increased G6PD activity after seven days of darkness.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- illuminated againe0
- Gene
- G6PDe0
- Outcome
- G6PD activitye0
- Direction
- increased rapidlye0
- Qualifier
- 7 days in the darke0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the callus cells were illuminated again after darkincubation, G6PD activity increased rapidly even in the cells cultured for 7 days in the dark.”
t0“Tobacco”
Claim · model-written summary
Longer dark incubation reduced the increase in G6PD activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- dark-incubation periode0
- Outcome
- degree of the increasee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The degree of the increase, however, reduced in proportion to the length of the dark-incubation period.”
t0“Tobacco”
Claim · model-written summary
The authors infer a chloroplast light acceptor induces G6PD increase.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- G6PDe0
- Outcome
- increase of G6PDe0
- Qualifier
- some acceptor of light in chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is some acceptor of light in chloroplasts, which induces the increase of G6PD”
t0“Tobacco”
Claim · model-written summary
Light did not enhance G6PD activity in white tobacco callus XD-6.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- XD-6e0
- Treatment
- lighte0
- Gene
- G6PDe0
- Outcome
- G6PD activitye0
- Direction
- not enhancede0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G6PD activity in white tobacco callus (XD-6) tissue, which can grow actively even in the dark and whose growth is not promoted by light, was not enhanced by light.”
Claim · model-written summary
White tobacco callus XD-6 grows actively in darkness.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- XD-6e0
- Outcome
- grow actively even in the darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“white tobacco callus (XD-6) tissue, which can grow actively even in the dark”
Claim · model-written summary
Light does not promote growth of white tobacco callus XD-6.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- XD-6e0
- Treatment
- lighte0
- Outcome
- growthe0
- Direction
- not promotede0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“white tobacco callus (XD-6) tissue, which can grow actively even in the dark and whose growth is not promoted by light”
Claim · model-written summary
Light enhancement of G6PD activity is specific to green callus enzyme.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- G6PDe0
- Outcome
- Enhancement of G6PD activity by lighte0
- Qualifier
- specific for the green callus enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhancement of G6PD activity by light is specific for the green callus enzyme”
t0“Tobacco”
Claim · model-written summary
Light-induced G6PD activity is inferred to contribute to photomixotrophic growth.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- photomixotrophic growthe0
- Qualifier
- it contributese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is inferred that it contributes to the photomixotrophic growth of the green tobacco callus tissue.”
The same questions in other papers
light · growth · Nicotiana tabacum (tobacco)Positive and negative results
17 papers in the same direction as this paper · 29 reporting no difference from an alternative
- positive
- positive
- positive
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.