Abstract
Plant viruses disrupt host metabolic pathways to facilitate infection. Sucrose metabolism may modulate viral resistance, yet the mechanism remains unclear. We used label-free proteomics to investigate sucrose phosphate synthase (SPS) activity in Nicotiana benthamiana during potato virus Y (PVY) infection. We identified 464 differentially expressed proteins enriched in SPS activity, amino acid metabolism, and carbohydrate biosynthesis. Functional analysis demonstrated that PVY infection significantly upregulated NbSPS3 expression and enhanced SPS activity and sucrose accumulation, which reduced viral accumulation. Silencing NbSPS3 compromised sucrose synthesis and increased PVY accumulation, confirming an antiviral defense role. NbSPS3 specifically interacted with the 14-3-3 protein NbGF14a, a positive regulator of PVY resistance. Silencing NbGF14a also enhanced susceptibility to PVY, suggesting a coordinated defense mechanism involving sucrose metabolism and 14-3-3 signaling. NbSPS3 -mediated sucrose biosynthesis was revealed as a critical metabolic defense strategy against PVY, offering new insights into plant-virus interactions and potential targets for engineering crop resistance.
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Claims 7
Claim · model-written summary
Proteomics identified 464 differentially expressed proteins enriched in SPS activity and metabolism.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Outcome
- differentially expressed proteinse0
- Value
- 464e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified 464 differentially expressed proteins enriched in SPS activity, amino acid metabolism, and carbohydrate biosynthesis”
t0“Nicotiana benthamiana”
Claim · model-written summary
PVY infection upregulated NbSPS3 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- PVY
Potato virus Ye0pathogen - Gene
- NbSPS3e0
- Outcome
- NbSPS3 expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY infection significantly upregulated NbSPS3 expression”
t0“Nicotiana benthamiana”
Claim · model-written summary
PVY infection enhanced SPS activity and sucrose accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- SPS activity and sucrose accumulatione0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY infection significantly upregulated NbSPS3 expression and enhanced SPS activity and sucrose accumulation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbSPS3 compromised sucrose synthesis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing NbSPS3e0
- Gene
- NbSPS3e0
- Outcome
- sucrose synthesise0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing NbSPS3 compromised sucrose synthesis and increased PVY accumulation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbSPS3 increased PVY accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing NbSPS3e0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- PVY accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing NbSPS3 compromised sucrose synthesis and increased PVY accumulation”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbSPS3 specifically interacted with NbGF14a.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbSPS3e0
- Outcome
- interacted with the 14-3-3 protein NbGF14ae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSPS3 specifically interacted with the 14-3-3 protein NbGF14a”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbGF14a increased susceptibility to PVY.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing NbGF14ae0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- susceptibility to PVYe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing NbGF14a also enhanced susceptibility to PVY”
t0“Nicotiana benthamiana”
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