Abstract
While excessive nitrogen fertilizer application enhances crop yields, it does so at the expense of ecosystem health, making the enhancement of nitrogen use efficiency (NUE) an imperative for sustainable agriculture. This study investigates the mechanisms underlying the response to low nitrate (LN) stress in two Brassica juncea genotypes: a high-NUE (H158) and a low-NUE (L159), aiming to identify genetic resources for improved NUE. Hydroponic experiments revealed that H158 displayed enhanced antioxidant capacity, nitrogen assimilation, and nitrogen uptake efficiency (NUpE) under LN conditions relative to L159. Field trials corroborated these findings, with H158 demonstrating higher yields and greater agronomic nitrogen use efficiency (ANUE) across a gradient of nitrogen application rates. Genomic analysis identified 1,654 genes associated with NUE, including NIN-LIKE PROTEIN 4 ( BjuB04 . NLP4 ), whose G/C variants influenced transcriptional regulation and root-to-shoot ratio responses to nitrogen availability. Notably, BjuB04 . NLP4 -HAP2 (H158) was predominant in low-nitrogen soils, while BjuB04 . NLP4 -HAP1 (L159) was more common in high-nitrogen soils. Collectively, our findings uncover and characterize valuable genetic resources for breeding rapeseed varieties with enhanced NUE, providing both elite germplasm and functional molecular markers.
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Claims 8
Claim · model-written summary
Under low nitrate conditions H158 showed enhanced antioxidant capacity, nitrogen assimilation and nitrogen uptake efficiency relative to L159.
- Taxon
- Brassica juncea
Brassica junceat0 - Material
- H158e1
- Treatment
- LN conditionse1
- Outcome
- antioxidant capacity, nitrogen assimilation, and nitrogen uptake efficiencye1
- Direction
- enhancede1
- Comparator
- L159e1
- Setting
- Hydroponice1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e1“Hydroponic experiments revealed that H158 displayed enhanced antioxidant capacity, nitrogen assimilation, and nitrogen uptake efficiency (NUpE) under LN conditions relative to L159”
Claim · model-written summary
In field trials H158 had higher yields across a gradient of nitrogen application rates.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- H158e2
- Treatment
- a gradient of nitrogen application ratese2
- Outcome
- yieldse2
- Direction
- highere2
- Setting
- Field trialse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e2“Field trials corroborated these findings, with H158 demonstrating higher yields and greater agronomic nitrogen use efficiency (ANUE) across a gradient of nitrogen application rates”
Claim · model-written summary
In field trials H158 had greater agronomic nitrogen use efficiency (ANUE) across a gradient of nitrogen application rates.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- H158e2
- Treatment
- a gradient of nitrogen application ratese2
- Outcome
- agronomic nitrogen use efficiency (ANUE)e2
- Direction
- greatere2
- Setting
- Field trialse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e2“Field trials corroborated these findings, with H158 demonstrating higher yields and greater agronomic nitrogen use efficiency (ANUE) across a gradient of nitrogen application rates”
Claim · model-written summary
Genomic analysis identified 1,654 genes associated with NUE.
- Taxon
- Brassica junceaBrassica junceat0
- Outcome
- genes associated with NUEe3
- Value
- 1,654 genese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e3“Genomic analysis identified 1,654 genes associated with NUE, including NIN-LIKE PROTEIN 4 ( BjuB04 . NLP4 ), whose G/C variants influenced transcriptional regulation and root-to-shoot ratio responses to nitrogen availability”
Claim · model-written summary
G/C variants of BjuB04 . NLP4 influenced transcriptional regulation and root-to-shoot ratio responses to nitrogen availability.
- Taxon
- Brassica junceaBrassica junceat0
- Treatment
- nitrogen availabilitye3
- Gene
- BjuB04 . NLP4e3
- Outcome
- transcriptional regulation and root-to-shoot ratio responsese3
- Qualifier
- G/C variantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e3“Genomic analysis identified 1,654 genes associated with NUE, including NIN-LIKE PROTEIN 4 ( BjuB04 . NLP4 ), whose G/C variants influenced transcriptional regulation and root-to-shoot ratio responses to nitrogen availability”
Claim · model-written summary
The BjuB04 . NLP4-HAP2 haplotype (H158) was predominant in low-nitrogen soils.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- H158e4
- Gene
- BjuB04 . NLP4 -HAP2e4
- Outcome
- predominante4
- Setting
- low-nitrogen soilse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e4“Notably, BjuB04 . NLP4 -HAP2 (H158) was predominant in low-nitrogen soils, while BjuB04 . NLP4 -HAP1 (L159) was more common in high-nitrogen soils”
Claim · model-written summary
The BjuB04 . NLP4-HAP1 haplotype (L159) was more common in high-nitrogen soils.
- Taxon
- Brassica junceaBrassica junceat0
- Material
- L159e4
- Gene
- BjuB04 . NLP4 -HAP1e4
- Outcome
- more commone4
- Setting
- high-nitrogen soilse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e4“Notably, BjuB04 . NLP4 -HAP2 (H158) was predominant in low-nitrogen soils, while BjuB04 . NLP4 -HAP1 (L159) was more common in high-nitrogen soils”
Claim · model-written summary
The study provides elite germplasm and functional molecular markers for breeding rapeseed with enhanced NUE (author interpretation).
Not reportedComparator
Verbatim
t0“divergent nitrogen use efficiency in Brassica juncea”
e5“Collectively, our findings uncover and characterize valuable genetic resources for breeding rapeseed varieties with enhanced NUE, providing both elite germplasm and functional molecular markers”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.