Abstract
Abstract In deciduous woody plants, buds enter endodormancy in late autumn, and endodormancy release (EDR) is a essential for regrowth. In tree peony, endodormancy is a major obstacle to forcing culture, an important component of its industry. Both chilling accumulation and gibberellin acid (GA) application can contribute to break dormancy and facilitate bud sprouting and flowering under appropriate conditions. PsmiR159b is one of the most abundant miRNAs in dormant buds, targeting PsMYB65 to inhibit bud EDR. Here, PsGA2ox2 was identified as a novel target of PsmiR159b according to expression data and genetic analysis. PsGA2ox2 expression was up-regulated by prolonged chilling, following with an increase of gibberellic acid 34 (GA 34 ), a product of deactivated GA 4 by GA2-oxidase. Silencing of PsGA2ox2 accelerated budbreak, accompanied by the increase of GA 1 and GA 4 , downregulation of PsABI5 , PsSVP , and upregulation of PsEBB1 , PsEBB3 , and PsCYCD . The content of GA 4 was downregulated in STTM159b buds, whereas increased in MIR159b-OE buds. In addition, the ectopic expression of PsGA2ox2 in Arabidopsis dramatically delayed seed germination and inhibited plant growth. These findings indicated a dual-target regulatory role for PsmiR159b in regulating GA homeostasis, and also revealed a PsmiR159b- PsGA2ox2 module that influenced EDR in tree peony through bioactive GA level regulation.
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Claims 13
Claim · model-written summary
PsGA2ox2 was identified as a novel target of PsmiR159b from expression data and genetic analysis in tree peony.
- Taxon
- tree peony
Paeonia suffruticosat0 - Gene
- PsGA2ox2e1
- Outcome
- novel target of PsmiR159be1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“PsGA2ox2 was identified as a novel target of PsmiR159b according to expression data and genetic analysis”
Claim · model-written summary
PsGA2ox2 expression was up-regulated by prolonged chilling in tree peony buds.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- prolonged chillinge1
- Gene
- PsGA2ox2e1
- Outcome
- PsGA2ox2 expressione1
- Direction
- up-regulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“PsGA2ox2 expression was up-regulated by prolonged chilling, following with an increase of gibberellic acid 34 (GA 34 )”
Claim · model-written summary
Gibberellic acid 34 increased following prolonged chilling in tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- prolonged chillinge1
- Outcome
- gibberellic acid 34 (GA 34 )e1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“PsGA2ox2 expression was up-regulated by prolonged chilling, following with an increase of gibberellic acid 34 (GA 34 )”
Claim · model-written summary
Silencing of PsGA2ox2 accelerated budbreak in tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Silencing of PsGA2ox2e1
- Gene
- PsGA2ox2e1
- Outcome
- budbreake1
- Direction
- acceleratede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“Silencing of PsGA2ox2 accelerated budbreak”
Claim · model-written summary
Silencing of PsGA2ox2 increased GA1 and GA4 levels in tree peony buds.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Silencing of PsGA2ox2e1
- Gene
- PsGA2ox2e1
- Outcome
- GA 1 and GA 4e1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“Silencing of PsGA2ox2 accelerated budbreak, accompanied by the increase of GA 1 and GA 4”
Claim · model-written summary
Silencing of PsGA2ox2 downregulated PsABI5 and PsSVP in tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Silencing of PsGA2ox2e1
- Gene
- PsABI5e1
- Outcome
- PsABI5 , PsSVPe1
- Direction
- downregulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“Silencing of PsGA2ox2 accelerated budbreak, accompanied by the increase of GA 1 and GA 4 , downregulation of PsABI5 , PsSVP”
Claim · model-written summary
Silencing of PsGA2ox2 upregulated PsEBB1, PsEBB3 and PsCYCD in tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- Silencing of PsGA2ox2e1
- Gene
- PsEBB1e2
- Outcome
- PsEBB1 , PsEBB3 , and PsCYCDe2
- Direction
- upregulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“Silencing of PsGA2ox2 accelerated budbreak”
e2“upregulation of PsEBB1 , PsEBB3 , and PsCYCD”
Claim · model-written summary
GA4 content was downregulated in STTM159b buds of tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- STTM159be1
- Outcome
- content of GA 4e1
- Direction
- downregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“The content of GA 4 was downregulated in STTM159b buds, whereas increased in MIR159b-OE buds”
Claim · model-written summary
GA4 content was increased in MIR159b-OE buds of tree peony.
- Taxon
- tree peonyPaeonia suffruticosat0
- Treatment
- MIR159b-OEe1
- Outcome
- content of GA 4e1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“The content of GA 4 was downregulated in STTM159b buds, whereas increased in MIR159b-OE buds”
Claim · model-written summary
Ectopic expression of PsGA2ox2 in Arabidopsis dramatically delayed seed germination.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- ectopic expression of PsGA2ox2e1
- Gene
- PsGA2ox2e1
- Outcome
- seed germinatione1
- Direction
- delayede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the ectopic expression of PsGA2ox2 in Arabidopsis dramatically delayed seed germination and inhibited plant growth”
Claim · model-written summary
Ectopic expression of PsGA2ox2 in Arabidopsis inhibited plant growth.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ectopic expression of PsGA2ox2e1
- Gene
- PsGA2ox2e1
- Outcome
- plant growthe1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the ectopic expression of PsGA2ox2 in Arabidopsis dramatically delayed seed germination and inhibited plant growth”
Claim · model-written summary
The authors conclude PsmiR159b has a dual-target regulatory role in regulating GA homeostasis.
- Taxon
- tree peonyPaeonia suffruticosat0
- Gene
- PsmiR159be1
- Outcome
- GA homeostasise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tree peony”
e1“These findings indicated a dual-target regulatory role for PsmiR159b in regulating GA homeostasis”
Claim · model-written summary
A PsmiR159b-PsGA2ox2 module influences endodormancy release in tree peony through regulation of bioactive GA level.
- Taxon
- tree peonyPaeonia suffruticosae1
- Gene
- PsGA2ox2e1
- Compound
- bioactive GAe1
- Outcome
- EDRe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a PsmiR159b- PsGA2ox2 module that influenced EDR in tree peony through bioactive GA level regulation”
The same questions in other papers
loss of function · phenology · Paeonia suffruticosa (tree peony)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.