Paper · 2026 · Horticulturae

The Impact of Planting Density and Vegetative Duration on Yield Optimization and Cannabinoid Stability in Medicinal Cannabis Under Controlled-Environment Cultivation

Panagiotis Karnoutsos; Stratos Mallis; Eirini Sarrou; et al.

6
claims
13
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by

Abstract

Optimizing plant density and vegetative growth duration is important for improving productivity in controlled-environment medicinal cannabis cultivation. Although both factors strongly influence canopy development and yield, their combined effects under modern high-intensity LED lighting, and particularly their consequences for cannabinoid uniformity across the canopy, remain insufficiently characterized. This study examined how planting density and vegetative duration influence plant growth, yield, and cannabinoid concentration in Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions, high-intensity LED lighting and precision fertigation. Two vegetative durations (10 and 28 days) were evaluated in separate but identical controlled-environment chambers under broad-spectrum high-intensity LED lighting and automated precision fertigation on rockwool substrate. The 10-day regime compared 8, 14 and 18 plants m−2; the 28-day regime compared 6, 8 and 10 plants m−2. Each combination was replicated across two independent cultivation cycles, and because density levels differed between regimes, direct between-regime comparisons were restricted to the shared density of 8 plants m−2. Extending the vegetative phase from 10 to 28 days increased plant height, stem diameter and internodal length. Area-based yield increased strongly with density, reaching 1091 g m−2 at 18 plants m−2 under the 10-day regime and 1009 g m−2 at 10 plants m−2 under the 28-day regime. Apical biomass exceeded basal biomass, but total THC concentration did not differ significantly among planting densities, vegetative durations or canopy positions. Higher planting densities combined with shorter vegetative periods can therefore increase area-based productivity while maintaining stable THC concentration under high-intensity LED cultivation.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Extending the vegetative phase from 10 to 28 days increased plant height, stem diameter and internodal length.

Taxon
Cannabis sativa L.Cannabis sativae1
Material
Fat Bananae1
Treatment
Extending the vegetative phase from 10 to 28 dayse0
Outcome
plant height, stem diameter and internodal lengthe0
Direction
increasede0
Setting
controlled-environment chamberse2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Extending the vegetative phase from 10 to 28 days increased plant height, stem diameter and internodal length”

✓ located verbatim in the source · abstract, characters 1224–1333 · show in text ↑
e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e2

“Two vegetative durations (10 and 28 days) were evaluated in separate but identical controlled-environment chambers”

✓ located verbatim in the source · abstract, characters 681–795 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Area-based yield of Cannabis sativa increased strongly with planting density.

Taxon
Cannabis sativa L.Cannabis sativae1
Treatment
densitye3
Outcome
Area-based yielde3
Direction
increasede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e3

“Area-based yield increased strongly with density, reaching 1091 g m−2 at 18 plants m−2 under the 10-day regime and 1009 g m−2 at 10 plants m−2 under the 28-day regime”

✓ located verbatim in the source · abstract, characters 1335–1501 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Area-based yield reached 1091 g m−2 at 18 plants m−2 under the 10-day regime.

Taxon
Cannabis sativa L.Cannabis sativae1
Treatment
18 plants m−2e3
Outcome
Area-based yielde3
Value
1091 g m−2e3
Qualifier
under the 10-day regimee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e3

“Area-based yield increased strongly with density, reaching 1091 g m−2 at 18 plants m−2 under the 10-day regime and 1009 g m−2 at 10 plants m−2 under the 28-day regime”

✓ located verbatim in the source · abstract, characters 1335–1501 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Area-based yield reached 1009 g m−2 at 10 plants m−2 under the 28-day regime.

Taxon
Cannabis sativa L.Cannabis sativae1
Treatment
10 plants m−2e3
Outcome
Area-based yielde3
Value
1009 g m−2e3
Qualifier
under the 28-day regimee3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e3

“Area-based yield increased strongly with density, reaching 1091 g m−2 at 18 plants m−2 under the 10-day regime and 1009 g m−2 at 10 plants m−2 under the 28-day regime”

✓ located verbatim in the source · abstract, characters 1335–1501 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Apical biomass exceeded basal biomass in Cannabis sativa.

Taxon
Cannabis sativa L.Cannabis sativae1
Outcome
Apical biomasse4
Direction
exceedede4
Comparator
basal biomasse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e4

“Apical biomass exceeded basal biomass”

✓ located verbatim in the source · abstract, characters 1503–1540 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Total THC concentration did not differ significantly among planting densities, vegetative durations or canopy positions.

Taxon
Cannabis sativa L.Cannabis sativae1
Treatment
planting densities, vegetative durations or canopy positionse5
Outcome
total THC concentratione5
Direction
did not differe5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions”

✓ located verbatim in the source · abstract, characters 538–624 · show in text ↑
e5

“total THC concentration did not differ significantly among planting densities, vegetative durations or canopy positions”

✓ located verbatim in the source · abstract, characters 1546–1665 · show in text ↑

density · molecular: metabolites · Cannabis sativaPositive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. no effect
See all 4 papers →

density · yield · Cannabis sativaPositive and negative results

9 papers: 7 positive, 2 negative or no effect

6 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.