Abstract
Sharka, caused by Potyvirus plumpoxi (plum pox virus, PPV), is the most destructive viral disease affecting Prunus species worldwide. Two MATH domain-containing genes, ParPMC1 and ParPMC2, have been identified in apricot as host susceptibility factors required for PPV infection, but their molecular function remains unclear. Here, we investigated ParPMC functions by identifying their interacting partners and assessing their contribution to viral replication and movement. Yeast two-hybrid screening of PPV-infected apricot tissues revealed 13 ParPMC interactors involved in defence and stress responses, primary metabolism, protein folding and gene expression. Among them, two eukaryotic elongation factor 1-α proteins (eEF1A; Pruar1 and Pruar9) were selected for further analysis. In planta assays showed that both ParPMC proteins colocalize with eEF1A at endoplasmic reticulum-derived perinuclear structures associated with viral replication complexes; however, a specific physical interaction was detected only for ParPMC1, suggesting paralog specialisation. Phylogenetic analyses revealed independent diversification of eEF1A gene families in Rosaceae and Solanaceae. Despite the lack of strict orthology, silencing clade B eEF1A isoforms in Nicotiana benthamiana using a cucumber mosaic virus-based virus-induced gene silencing system strongly reduced PPV accumulation and systemic spread, highlighting a conserved proviral role. These results support a model in which ParPMC1 facilitates PPV infection by recruiting or stabilising eEF1A at viral replication sites, whereas ParPMC2 may contribute through alternative interactors. This study advances our understanding of ParPMC-mediated PPV-susceptibility in apricot and highlights host-virus interactions with potential relevance for resistance strategies in Prunus and other potyvirus-susceptible crops.
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Claims 8
Claim · model-written summary
Screening found 13 ParPMC interactors in infected apricot tissues.
- Taxon
- apricot
Prunus armeniacae0 - Interactor
- PPVe0pathogen
- Gene
- ParPMCe0
- Outcome
- ParPMC interactorse0
- Value
- 13e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yeast two-hybrid screening of PPV-infected apricot tissues revealed 13 ParPMC interactors”
Claim · model-written summary
Both ParPMC proteins colocalized with eEF1A at structures associated with viral replication.
- Taxon
- apricotPrunus armeniacae1
- Gene
- ParPMC proteinse0
- Outcome
- colocalize with eEF1Ae0
- Qualifier
- endoplasmic reticulum-derived perinuclear structurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both ParPMC proteins colocalize with eEF1A at endoplasmic reticulum-derived perinuclear structures associated with viral replication complexes”
e1“apricot tissues”
Claim · model-written summary
Specific physical interaction with eEF1A was detected only for ParPMC1.
- Taxon
- apricotPrunus armeniacae1
- Gene
- ParPMC1e0
- Outcome
- specific physical interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a specific physical interaction was detected only for ParPMC1”
e1“apricot tissues”
Claim · model-written summary
eEF1A gene families diversified independently in Rosaceae and Solanaceae.
- Taxon
- Rosaceae and SolanaceaeRosaceaeSolanaceaee0
- Gene
- eEF1Ae0
- Outcome
- independent diversificatione0
Verbatim
e0“Phylogenetic analyses revealed independent diversification of eEF1A gene families in Rosaceae and Solanaceae.”
Claim · model-written summary
Silencing clade B eEF1A reduced PPV accumulation in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- silencing clade B eEF1A isoformse0
- Interactor
- PPVe0pathogen
- Gene
- clade B eEF1A isoformse0
- Outcome
- PPV accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing clade B eEF1A isoforms in Nicotiana benthamiana using a cucumber mosaic virus-based virus-induced gene silencing system strongly reduced PPV accumulation and systemic spread”
Claim · model-written summary
Silencing clade B eEF1A reduced PPV systemic spread in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- silencing clade B eEF1A isoformse0
- Interactor
- PPVe0pathogen
- Gene
- clade B eEF1A isoformse0
- Outcome
- PPV accumulation and systemic spreade0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing clade B eEF1A isoforms in Nicotiana benthamiana using a cucumber mosaic virus-based virus-induced gene silencing system strongly reduced PPV accumulation and systemic spread”
Claim · model-written summary
Authors proposed ParPMC1 facilitates PPV infection by recruiting or stabilizing eEF1A.
- Taxon
- apricotPrunus armeniacae1
- Interactor
- PPVe0pathogen
- Gene
- ParPMC1e0
- Outcome
- PPV infectione0
- Qualifier
- recruiting or stabilising eEF1Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ParPMC1 facilitates PPV infection by recruiting or stabilising eEF1A at viral replication sites”
e1“apricot tissues”
Claim · model-written summary
Authors proposed ParPMC2 contributes through alternative interactors.
- Taxon
- apricotPrunus armeniacae1
- Gene
- ParPMC2e0
- Outcome
- contribute through alternative interactorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ParPMC2 may contribute through alternative interactors”
e1“apricot tissues”
The same questions in other papers
loss of function · molecular: metabolites · Nicotiana benthamianaPositive and negative results
9 papers in the same direction as this paper · 25 in the opposite direction
- negative
- negative
- no effect
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