Abstract
The biocontrol potential of Methylobacterium spp. against key phytopathogenic fungi was evaluated using in vitro antagonism assays and confined germination bioassays. Across 40 Methylobacterium isolates, inhibitory activity was highly variable and strain-dependent. Fusarium graminearum (UAMH 3329) was the most consistently suppressed pathogen; however, only M. thiocyanatum (NBRC 103124) and M. aminovorans (LMG 21752) produced significant inhibition (>30%, p < 0.05) across all three Fusarium species tested. Growth suppression was enhanced on nutrient-minimum agar amended with cell-free bacterial broth, supporting the involvement of diffusible inhibitory factors in fungal growth suppression. However, marked isolate- and substrate-dependent effects suggest that antagonism is contingent on specific bacterium–pathogen combinations. In germination assays, M. organophilum (LMG 6083) conferred protection to soybean ( Glycine max) seeds challenged with F. graminearum, improving germination and seedling development in both artificial and soil substrates. Co-inoculated seeds exhibited lower disease severity scores (DSS), fewer necrotic lesions, and reduced fungal colonization during germination and emergence. Collectively, these findings identify candidate Methylobacterium strains with antagonistic activity against Fusarium spp. and provide a foundation for future characterization of their effectors relevant to integrated crop protection.
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Claims 9
Claim · model-written summary
M. thiocyanatum (NBRC 103124) significantly inhibited all three Fusarium species tested by more than 30%.
- Taxon
- soybean
Glycine maxe2 - Second interactor
- M. thiocyanatum (NBRC 103124)e1biocontrol_agent
- Outcome
- inhibitione1
- Value
- >30%e1
FusariumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“only M. thiocyanatum (NBRC 103124) and M. aminovorans (LMG 21752) produced significant inhibition (>30%, p < 0.05) across all three Fusarium species tested”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
M. aminovorans (LMG 21752) significantly inhibited all three Fusarium species tested by more than 30%.
- Taxon
- soybeanGlycine maxe2
- Second interactor
- M. aminovorans (LMG 21752)Methylorubrum aminovoranse1biocontrol_agent
- Outcome
- inhibitione1
- Value
- >30%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“only M. thiocyanatum (NBRC 103124) and M. aminovorans (LMG 21752) produced significant inhibition (>30%, p < 0.05) across all three Fusarium species tested”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
Fusarium graminearum (UAMH 3329) was the most consistently suppressed of the tested pathogens.
- Taxon
- soybeanGlycine maxe2
- Interactor
- Fusarium graminearum (UAMH 3329)
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume3pathogen - Outcome
- suppressede3
- Qualifier
- most consistentlye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e3“Fusarium graminearum (UAMH 3329) was the most consistently suppressed pathogen”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
Inhibitory activity of the 40 Methylobacterium isolates was highly variable and strain-dependent.
- Taxon
- soybeanGlycine maxe2
- Material
- Methylobacterium isolatese4
- Outcome
- inhibitory activitye4
- Qualifier
- highly variable and strain-dependente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Across 40 Methylobacterium isolates, inhibitory activity was highly variable and strain-dependent”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
Growth suppression was enhanced on nutrient-minimum agar amended with cell-free bacterial broth.
- Taxon
- soybeanGlycine maxe2
- Treatment
- cell-free bacterial brothe5
- Outcome
- Growth suppressione5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Growth suppression was enhanced on nutrient-minimum agar amended with cell-free bacterial broth”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
M. organophilum (LMG 6083) protected soybean seeds challenged with F. graminearum, improving germination and seedling development in artificial and soil substrates.
- Taxon
- soybeanGlycine maxe6
- Treatment
- M. organophilum (LMG 6083)e6
- Interactor
- F. graminearumFusarium graminearume6pathogen
- Second interactor
- M. organophilum (LMG 6083)Methylobacterium organophilume6biocontrol_agent
- Outcome
- germination and seedling developmente6
- Direction
- improvinge6
- Setting
- artificial and soil substratese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“M. organophilum (LMG 6083) conferred protection to soybean ( Glycine max) seeds challenged with F. graminearum, improving germination and seedling development in both artificial and soil substrates”
Claim · model-written summary
Co-inoculated soybean seeds had lower disease severity scores (DSS).
- Taxon
- soybeanGlycine maxe2
- Treatment
- Co-inoculated seedse7
- Outcome
- disease severity scores (DSS)e7
- Direction
- lowere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Co-inoculated seeds exhibited lower disease severity scores (DSS), fewer necrotic lesions, and reduced fungal colonization during germination and emergence”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
Co-inoculated soybean seeds had fewer necrotic lesions.
- Taxon
- soybeanGlycine maxe2
- Treatment
- Co-inoculated seedse7
- Outcome
- necrotic lesionse7
- Direction
- fewere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Co-inoculated seeds exhibited lower disease severity scores (DSS), fewer necrotic lesions, and reduced fungal colonization during germination and emergence”
e2“soybean ( Glycine max) seeds”
Claim · model-written summary
Co-inoculated soybean seeds had reduced fungal colonization during germination and emergence.
- Taxon
- soybeanGlycine maxe2
- Treatment
- Co-inoculated seedse7
- Outcome
- fungal colonizatione7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Co-inoculated seeds exhibited lower disease severity scores (DSS), fewer necrotic lesions, and reduced fungal colonization during germination and emergence”
e2“soybean ( Glycine max) seeds”
The same questions in other papers
inoculation · disease · Glycine max (soybean)Positive and negative results
59 papers in the same direction as this paper · 67 in the opposite direction
- negative
- no effect
- negative
123 papers more in this group
See all 127 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.