Abstract
Plasmodiophora brassicae poses a severe threat to rapeseed (Brassica napus) production worldwide. As an obligate biotrophic pathogen, it deploys effectors to suppress host immunity, yet how these effectors manipulate plant defence, particularly salicylic acid (SA)-b-mediated immunity, remains largely unknown. In this study, a CBM1 (carbohydrate-binding module)-containing effector, PbCBM1, from P. brassicae was identified, which is secreted and suppresses INF1/BAX-induced cell death. PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae. AtPHB3 (Prohibitin 3), a key regulator of SA biosynthesis, was identified as the interactor of PbCBM1 both in vivo and in vitro. The phb3 mutant also exhibited increased susceptibility, suggesting that PbCBM1 may promote plant susceptibility by targeting AtPHB3 during pathogen infection. The subsequent results showed that PbCBM1-overexpressing plants reduced SA pathway gene expression and isochorismate/SA accumulation upon chitin treatment. Furthermore, LUC and competitive Co-IP assays revealed that PbCBM1 competes with AtICS1 (Isochorismate synthase 1) for AtPHB3 binding. Collectively, P. brassicae secretes the effector PbCBM1 to competitively target AtPHB3, disrupting AtPHB3-AtICS1 complex integrity, thereby suppressing SA biosynthesis and immunity. This study reveals a novel molecular mechanism by which a biotrophic pathogen subverts plant defence and provides potential targets for breeding clubroot-resistant rapeseed varieties.
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Claims 9
Claim · model-written summary
The P. brassicae effector PbCBM1 suppresses INF1/BAX-induced cell death.
- Taxon
- rapeseed (Brassica napus)
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Interactor
- P. brassicae
.jpg?width=60)
Plasmodiophora brassicaee1pathogen - Gene
- PbCBM1e1
- Outcome
- INF1/BAX-induced cell deathe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
.jpg?width=500)
Verbatim
e0“Plasmodiophora brassicae poses a severe threat to rapeseed (Brassica napus) production worldwide”
e1“a CBM1 (carbohydrate-binding module)-containing effector, PbCBM1, from P. brassicae was identified, which is secreted and suppresses INF1/BAX-induced cell death”
Claim · model-written summary
Arabidopsis overexpressing PbCBM1 showed significantly elevated susceptibility to P. brassicae.
- Taxon
- Arabidopsis
Arabidopsise2 - Treatment
- PbCBM1-overexpressinge2
- Interactor
- P. brassicaePlasmodiophora brassicaee2pathogen
- Outcome
- susceptibility to P. brassicaee2
- Direction
- elevatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
Claim · model-written summary
The Arabidopsis phb3 mutant showed increased susceptibility to P. brassicae.
- Taxon
- ArabidopsisArabidopsise2
- Material
- phb3 mutante4
- Treatment
- phb3 mutante4
- Interactor
- P. brassicaePlasmodiophora brassicaee2pathogen
- Outcome
- susceptibilitye4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e4“The phb3 mutant also exhibited increased susceptibility”
Claim · model-written summary
AtPHB3 was identified as an interactor of PbCBM1 in vivo and in vitro in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- AtPHB3e3
- Outcome
- interactor of PbCBM1e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e3“AtPHB3 (Prohibitin 3), a key regulator of SA biosynthesis, was identified as the interactor of PbCBM1 both in vivo and in vitro”
Claim · model-written summary
PbCBM1-overexpressing plants reduced salicylic acid pathway gene expression upon chitin treatment.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- PbCBM1-overexpressinge5
- Outcome
- SA pathway gene expressione5
- Direction
- reducede5
- Qualifier
- upon chitin treatmente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e5“PbCBM1-overexpressing plants reduced SA pathway gene expression and isochorismate/SA accumulation upon chitin treatment”
Claim · model-written summary
PbCBM1-overexpressing plants reduced isochorismate/SA accumulation upon chitin treatment.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- PbCBM1-overexpressinge5
- Outcome
- isochorismate/SA accumulatione5
- Direction
- reducede5
- Qualifier
- upon chitin treatmente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e5“PbCBM1-overexpressing plants reduced SA pathway gene expression and isochorismate/SA accumulation upon chitin treatment”
Claim · model-written summary
PbCBM1 competes with AtICS1 for AtPHB3 binding in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- AtICS1e6
- Outcome
- AtPHB3 bindinge6
- Qualifier
- competes with AtICS1e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e6“PbCBM1 competes with AtICS1 (Isochorismate synthase 1) for AtPHB3 binding”
Claim · model-written summary
P. brassicae effector PbCBM1 disrupts the AtPHB3-AtICS1 complex and thereby suppresses salicylic acid biosynthesis and immunity in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Interactor
- P. brassicaePlasmodiophora brassicaee7pathogen
- Gene
- AtPHB3-AtICS1e7
- Outcome
- SA biosynthesis and immunitye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PbCBM1-overexpressing Arabidopsis showed significantly elevated susceptibility to P. brassicae”
e7“P. brassicae secretes the effector PbCBM1 to competitively target AtPHB3, disrupting AtPHB3-AtICS1 complex integrity, thereby suppressing SA biosynthesis and immunity”
Claim · model-written summary
The authors suggest that the PbCBM1-AtPHB3 pathway provides potential targets for breeding clubroot-resistant rapeseed varieties.
Verbatim
e7“provides potential targets for breeding clubroot-resistant rapeseed varieties”