Paper · 2026 · Agronomy

Preharvest Biostimulant–Calcium Application Enhances Blueberry Fruit Quality Through Structural and Cuticular Modifications

Tiago Lopes; Ana Paula Silva; Helena Ferreira; et al.

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verbatim quotes
0
citations
4
research areas
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Open access · cc-by

Abstract

The increased demand for higher-quality, longer-lasting blueberries has led to the development of preharvest strategies to improve their structural integrity sustainably. This study analysed the effects of the foliar application of two biostimulant–calcium (Ca) combinations, using Ecklonia maxima extract (EM + Ca) and glycine betaine (GB + Ca), on yield, biometric, mechanical, and histological properties, as well as cuticular wax composition of blueberries. Both biostimulants increased yield per plant and fruit weight and size in ‘Duke’, with superior results for GB + Ca. Fruit yield increased by 80% with GB + Ca and 40% with EM + Ca. Histological analysis showed increases in cuticle thickness, epidermal cell area and thickness, and hypodermal cell area and area/perimeter ratio. This thicker, denser tissue ultimately improved blueberries’ mechanical properties. Specifically, ‘Draper’ berries treated with GB + Ca had 36%, 15%, and 20% higher values for flesh firmness, stiffness, and deformation work, respectively, relative to the control. However, cuticular wax accumulation was more pronounced with EM + Ca for the ‘Duke’ cultivar, increasing by 12%. Overall, GB + Ca had the greatest impact on blueberry structural quality and may represent a promising strategy to improve postharvest quality and commercial production.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both biostimulants increased Duke yield per plant.

Taxon
Blueberryt0
Material
‘Duke’e0
Treatment
Both biostimulantse0
Outcome
yield per plante0
Direction
increasede0

Not reportedComparator

Verbatim

e0

“Both biostimulants increased yield per plant and fruit weight and size in ‘Duke’, with superior results for GB + Ca”

✓ located verbatim in the source · abstract, characters 462–577 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both biostimulants increased Duke fruit weight and size.

Taxon
Blueberryt0
Material
‘Duke’e0
Treatment
Both biostimulantse0
Outcome
fruit weight and sizee0
Direction
increasede0

Not reportedComparator

Verbatim

e0

“Both biostimulants increased yield per plant and fruit weight and size in ‘Duke’, with superior results for GB + Ca”

✓ located verbatim in the source · abstract, characters 462–577 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GB plus calcium increased fruit yield by 80%.

Taxon
Blueberryt0
Treatment
GB + Cae0
Outcome
Fruit yielde0
Direction
increasede0
Value
80%e0

Not reportedComparator

Verbatim

e0

“Fruit yield increased by 80% with GB + Ca and 40% with EM + Ca”

✓ located verbatim in the source · abstract, characters 579–641 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

EM plus calcium increased fruit yield by 40%.

Taxon
Blueberryt0
Treatment
EM + Cae0
Outcome
Fruit yielde0
Direction
increasede0
Value
40%e0

Not reportedComparator

Verbatim

e0

“Fruit yield increased by 80% with GB + Ca and 40% with EM + Ca”

✓ located verbatim in the source · abstract, characters 579–641 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment increased cuticle thickness and epidermal cell area and thickness.

Taxon
Blueberryt0
Treatment
biostimulant–calciumt0
Outcome
cuticle thickness, epidermal cell area and thicknesse0
Direction
increasese0

Not reportedComparator

Verbatim

e0

“Histological analysis showed increases in cuticle thickness, epidermal cell area and thickness, and hypodermal cell area and area/perimeter ratio”

✓ located verbatim in the source · abstract, characters 643–788 · show in text ↑
t0

“Biostimulant–Calcium Application Enhances Blueberry”

✓ located verbatim in the source · title, characters 11–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treatment increased hypodermal cell area and area/perimeter ratio.

Taxon
Blueberryt0
Treatment
biostimulant–calciumt0
Outcome
hypodermal cell area and area/perimeter ratioe0
Direction
increasese0

Not reportedComparator

Verbatim

e0

“Histological analysis showed increases in cuticle thickness, epidermal cell area and thickness, and hypodermal cell area and area/perimeter ratio”

✓ located verbatim in the source · abstract, characters 643–788 · show in text ↑
t0

“Biostimulant–Calcium Application Enhances Blueberry”

✓ located verbatim in the source · title, characters 11–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GB plus calcium increased Draper flesh firmness by 36% relative to control.

Taxon
Blueberryt0
Material
‘Draper’e0
Treatment
GB + Cae0
Outcome
flesh firmnesse0
Direction
highere0
Value
36%e0
Comparator
the controle0

Verbatim

e0

“‘Draper’ berries treated with GB + Ca had 36%, 15%, and 20% higher values for flesh firmness, stiffness, and deformation work, respectively, relative to the control”

✓ located verbatim in the source · abstract, characters 888–1052 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GB plus calcium increased Draper stiffness by 15% relative to control.

Taxon
Blueberryt0
Material
‘Draper’e0
Treatment
GB + Cae0
Outcome
stiffnesse0
Direction
highere0
Value
15%e0
Comparator
the controle0

Verbatim

e0

“‘Draper’ berries treated with GB + Ca had 36%, 15%, and 20% higher values for flesh firmness, stiffness, and deformation work, respectively, relative to the control”

✓ located verbatim in the source · abstract, characters 888–1052 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GB plus calcium increased Draper deformation work by 20% relative to control.

Taxon
Blueberryt0
Material
‘Draper’e0
Treatment
GB + Cae0
Outcome
deformation worke0
Direction
highere0
Value
20%e0
Comparator
the controle0

Verbatim

e0

“‘Draper’ berries treated with GB + Ca had 36%, 15%, and 20% higher values for flesh firmness, stiffness, and deformation work, respectively, relative to the control”

✓ located verbatim in the source · abstract, characters 888–1052 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

EM plus calcium increased Duke cuticular wax accumulation by 12%.

Taxon
Blueberryt0
Material
‘Duke’e0
Treatment
EM + Cae0
Outcome
cuticular wax accumulatione0
Direction
increasinge0
Value
12%e0

Not reportedComparator

Verbatim

e0

“cuticular wax accumulation was more pronounced with EM + Ca for the ‘Duke’ cultivar, increasing by 12%”

✓ located verbatim in the source · abstract, characters 1063–1165 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑

calcium atom · yield · VacciniumPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

calcium atom · quality: postharvest · VacciniumPositive and negative results

5 papers: 2 positive, 3 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

1 paper more in this group

See all 5 papers →

glycine betaine · yield · VacciniumResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

glycine betaine · quality: postharvest · VacciniumResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

other · yield · VacciniumPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.