Abstract
ABSTRACT Soil salinity is a major abiotic stress that threatens global crop productivity. Calcium (Ca 2+ ) is a pivotal second messenger in plant stress signalling, but how Ca 2+ sensors such as calmodulin (CaM) transcriptionally control ion homeostasis and salt tolerance remains largely understood in crops. Here, we identified a novel calmodulin‐binding protein HvCBP60b, which is a negative regulator of salt resilience in salt‐tolerant barley. We showed that HvCBP60b interacts with Ca 2+ sensor HvCaM1 via its C‐terminal domain in a Ca 2+ ‐dependent manner. Loss of either HvCBP60b or HvCaM1 promoted the uptake of K + , increased the K + /Na + ratio, and markedly enhanced both growth performance and grain yield under salinity stress. Furthermore, HvCBP60b binds to the promoter of R2R3‐type MYB transcription factor HvMYB77 through its N‐terminal domain. HvMYB77 then transcriptionally reduces the expression of HvHAK5, a high‐affinity K + transporter essential for maintaining K + homeostasis under salinity. Mutations in HvMYB77 or HvHAK5 compromise salt tolerance, whereas the salt‐sensitive phenotype of hvhak5 is rescued in hvcbp60b‐hvhak5 double mutants. Therefore, we uncover a novel Ca 2+ ‐responsive regulatory module CaM1‐CBP60b‐MYB77‐HAK5 essential for K + homeostasis and salt tolerance in barley, highlighting new targets of Ca 2+ signalling for improving salt resilience in cereal crops.
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Claims 9
Claim · model-written summary
HvCBP60b, a calmodulin-binding protein, was identified as a negative regulator of salt resilience in salt-tolerant barley.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Gene
- HvCBP60be0
- Outcome
- negative regulator of salt resiliencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Barley”
e0“HvCBP60b, which is a negative regulator of salt resilience”
Claim · model-written summary
HvCBP60b interacts with the Ca2+ sensor HvCaM1 via its C-terminal domain in a Ca2+-dependent manner.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvCBP60be0
- Outcome
- interacts with Ca 2+ sensor HvCaM1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“We showed that HvCBP60b interacts with Ca 2+ sensor HvCaM1 via its C”
Claim · model-written summary
Loss of either HvCBP60b or HvCaM1 promoted K+ uptake and increased the K+/Na+ ratio.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Loss of either HvCBP60b or HvCaM1e0
- Gene
- HvCBP60b or HvCaM1e0
- Outcome
- the uptake of K + , increased the K + /Na + ratioe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“Loss of either HvCBP60b or HvCaM1 promoted the uptake of K + , increased the K + /Na + ratio”
Claim · model-written summary
Loss of either HvCBP60b or HvCaM1 markedly enhanced growth performance and grain yield under salinity stress.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Loss of either HvCBP60b or HvCaM1e0
- Gene
- HvCBP60b or HvCaM1e0
- Outcome
- growth performance and grain yielde1
- Direction
- enhancede1
- Qualifier
- under salinity stresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“Loss of either HvCBP60b or HvCaM1”
e1“markedly enhanced both growth performance and grain yield under salinity stress”
Claim · model-written summary
HvCBP60b binds to the promoter of the R2R3-type MYB transcription factor HvMYB77 through its N-terminal domain.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvCBP60be0
- Outcome
- binds to the promoter ofe0
- Qualifier
- HvMYB77e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“Furthermore, HvCBP60b binds to the promoter of R2R3”
e1“transcription factor HvMYB77”
Claim · model-written summary
HvMYB77 transcriptionally reduces the expression of the high-affinity K+ transporter gene HvHAK5.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvMYB77e0
- Outcome
- expression of HvHAK5e0
- Direction
- reducese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“HvMYB77 then transcriptionally reduces the expression of HvHAK5”
Claim · model-written summary
Mutations in HvMYB77 or HvHAK5 compromise salt tolerance.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Mutations in HvMYB77 or HvHAK5e0
- Gene
- HvMYB77 or HvHAK5e0
- Outcome
- salt tolerancee0
- Direction
- compromisee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“Mutations in HvMYB77 or HvHAK5 compromise salt tolerance”
Claim · model-written summary
The salt-sensitive phenotype of hvhak5 is rescued in hvcbp60b-hvhak5 double mutants.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- hvhak5e0
- Outcome
- phenotype of hvhak5 is rescuede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“phenotype of hvhak5 is rescued in hvcbp60b”
Claim · model-written summary
The CaM1-CBP60b-MYB77-HAK5 Ca2+-responsive regulatory module is proposed to be essential for K+ homeostasis and salt tolerance in barley.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- K + homeostasis and salt tolerancee0
- Qualifier
- essentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley”
e0“essential for K + homeostasis and salt tolerance in barley”
The same questions in other papers
loss of function · tolerance: abiotic · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.