Abstract
Biostimulant-based seed priming in Brassica napus offers a sustainable approach to improve crop establishment and stress resilience. We compared an Ascophyllum nodosum biostimulant (EpiSeed) with hydropriming and untreated controls in spring and winter oilseed rape varieties. We integrated laboratory assays, mannitol induced osmotic stress germination test, a single site field trial, and GC-MS metabolomics across four stages: after priming (AP), dry seeds, 6h re-imbibition under non-stress (6h-NS), and osmotic stress (6h-DS). EpiSeed priming significantly accelerated germination, enhanced early root growth and improved mannitol induced osmotic stress tolerance during germination compared with hydropriming and controls. In the field, Lagonda variety spring oilseed rape seeds primed with EpiSeed showed significantly faster seedling emergence, a non-significant trend in yield increase (15%), and reduced seed glucosinolate content (9%) in a single growing season. Metabolomics of the same variety identified 26 differentially accumulated metabolites. During priming, both treatments enriched alanine, aspartate, glutamate, cyanoamino acid, arginine, proline, and branched-chain amino acid (BCAA) metabolism pathways. During re-imbibition, responses diverged: under non-stress, EpiSeed showed coordinated metabolic utilization consistent with rapid growth and seed-to-seedling transition, whereas hydropriming altered mainly 4-aminobutanoic acid (GABA). Under osmotic stress, EpiSeed was associated with a more growth-oriented metabolic response via arginine and proline metabolism, while hydropriming exhibited a survival-oriented strategy dominated by BCAA and alanine, aspartate, glutamate pathways. Temporal trajectory analysis revealed a persistent EpiSeed specific metabolic signature marked by 4-hydroxyproline, consistent with cell wall related metabolism and growth activation. Collectively, EpiSeed establishes a distinct metabolic signature linking early biochemical reprogramming to improved germination, osmotic stress resilience, and field performance in oilseed rape.
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Claims 13
Claim · model-written summary
EpiSeed accelerated germination relative to hydropriming and untreated controls.
- Treatment
- EpiSeed priminge0
- Outcome
- germinatione0
- Direction
- acceleratede0
- Comparator
- hydropriming and controlse0
Not reportedTaxon
Verbatim
e0“EpiSeed priming significantly accelerated germination, enhanced early root growth and improved mannitol induced osmotic stress tolerance during germination compared with hydropriming and controls”
Claim · model-written summary
EpiSeed enhanced early root growth relative to comparators.
- Treatment
- EpiSeed priminge0
- Outcome
- early root growthe0
- Direction
- enhancede0
- Comparator
- hydropriming and controlse0
Not reportedTaxon
Verbatim
e0“EpiSeed priming significantly accelerated germination, enhanced early root growth and improved mannitol induced osmotic stress tolerance during germination compared with hydropriming and controls”
Claim · model-written summary
EpiSeed improved osmotic stress tolerance during germination.
- Treatment
- EpiSeed priminge0
- Outcome
- mannitol induced osmotic stress tolerancee0
- Direction
- improvede0
- Comparator
- hydropriming and controlse0
Not reportedTaxon
Verbatim
e0“EpiSeed priming significantly accelerated germination, enhanced early root growth and improved mannitol induced osmotic stress tolerance during germination compared with hydropriming and controls”
Claim · model-written summary
EpiSeed significantly hastened Lagonda seedling emergence in the field.
- Taxon
- spring oilseed rape
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Material
- Lagondae0
- Treatment
- primed with EpiSeede0
- Outcome
- seedling emergencee0
- Direction
- fastere0
- Setting
- fielde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“In the field, Lagonda variety spring oilseed rape seeds primed with EpiSeed showed significantly faster seedling emergence”
Claim · model-written summary
EpiSeed showed a non-significant 15% yield increase in the field.
Not reportedTaxonTreatmentComparator
Verbatim
e0“a non-significant trend in yield increase (15%)”
Claim · model-written summary
EpiSeed reduced seed glucosinolate content by 9%.
Not reportedTaxonTreatmentComparator
Verbatim
e0“reduced seed glucosinolate content (9%)”
Claim · model-written summary
Metabolomics identified 26 differentially accumulated metabolites in Lagonda.
Not reportedTaxon
Verbatim
e0“Metabolomics of the same variety identified 26 differentially accumulated metabolites”
Claim · model-written summary
Both priming treatments enriched several amino acid metabolism pathways.
Not reportedTaxon
Verbatim
e0“During priming, both treatments enriched alanine, aspartate, glutamate, cyanoamino acid, arginine, proline, and branched-chain amino acid (BCAA) metabolism pathways”
Claim · model-written summary
EpiSeed caused coordinated metabolic utilization during non-stress re-imbibition.
Not reportedTaxon
Verbatim
e0“under non-stress, EpiSeed showed coordinated metabolic utilization consistent with rapid growth and seed-to-seedling transition”
Claim · model-written summary
Hydropriming mainly altered GABA during non-stress re-imbibition.
Not reportedTaxon
Verbatim
e0“hydropriming altered mainly 4-aminobutanoic acid (GABA)”
Claim · model-written summary
Under osmotic stress, EpiSeed was associated with a growth-oriented arginine and proline response.
- Treatment
- EpiSeede0
- Outcome
- growth-oriented metabolic responsee0
- Direction
- moree0
- Qualifier
- Under osmotic stresse0
Not reportedTaxon
Verbatim
e0“Under osmotic stress, EpiSeed was associated with a more growth-oriented metabolic response via arginine and proline metabolism”
Claim · model-written summary
Hydropriming under osmotic stress showed a survival-oriented metabolic strategy.
- Treatment
- hydropriminge0
- Outcome
- survival-oriented strategye0
- Qualifier
- BCAA and alanine, aspartate, glutamate pathwayse0
Not reportedTaxon
Verbatim
e0“hydropriming exhibited a survival-oriented strategy dominated by BCAA and alanine, aspartate, glutamate pathways”
Claim · model-written summary
EpiSeed produced a persistent metabolic signature marked by 4-hydroxyproline.
Not reportedTaxon
Verbatim
e0“Temporal trajectory analysis revealed a persistent EpiSeed specific metabolic signature marked by 4-hydroxyproline”
The same questions in other papers
seed treatment · growth · Brassica napus (oilseed rape)Results differ
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