Paper · 2025 · Annals of Applied Biology

Rapeseed (Brassica napus L.) response to salinity and seed priming with NaCl

Christos A. Damalas; Spyridon D. Koutroubas

11
claims
21
verbatim quotes
3
citations
1
research areas
—
Not open access

Abstract

Abstract Laboratory and pot trials were conducted to study germination performance of rapeseed ( Brassica napus L., genotype Excalibur) under NaCl salinity after seed priming with 20 mM NaCl for 24 h. In the laboratory trial, salinity levels of 80, 160, and 240 mM NaCl reduced the germination percentage by 14, 56, and 80 percentage points, respectively compared to the non‐saline control. NaCl priming lowered the detrimental effect of salt stress on germination by promoting germination from 81% to 85% under salinity of 80 mM, from 39% to 68% under salinity of 160 mM, and from 15% to 56% under salinity of 240 mM NaCl (maximum alleviation). Furthermore, NaCl priming increased the germination tolerance index and the vigour index of seedlings as compared with the control seeds. Relative water content of the leaves was decreased with increasing salinity, showing limited water availability and a loss of turgor in the leaves. NaCl priming reduced the decrease in relative water content under salinity. Concerning the pot trial, the salinity level of 240 mM NaCl reduced the dry weight of rapeseed seedlings by 80.9% in the non‐primed seeds and by 55.8% in the primed seeds. NaCl priming improved the mean productivity index by 1.26 times under salinity of 240 mM NaCl and the harmonic mean index by 1.98 times under salinity of 240 mM NaCl compared with non‐primed seeds. Salinity limited seed performance of rapeseed, whereas NaCl priming improved seed performance and early growth in saline environment. The priming method needs to be examined in a selection of relevant genotypes under a range of saline conditions.

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Claims 11

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salinity at 80, 160, and 240 mM NaCl reduced germination by 14, 56, and 80 percentage points, respectively.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
salinity levels of 80, 160, and 240 mM NaCle0
Outcome
germination percentagee0
Direction
reducede0
Comparator
non‐saline controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“salinity levels of 80, 160, and 240 mM NaCl reduced the germination percentage by 14, 56, and 80 percentage points, respectively compared to the non‐saline control”

✓ located verbatim in the source · abstract, characters 226–389 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl priming promoted germination from 81% to 85% at 80 mM salinity.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
NaCl priminge0
Outcome
germinatione0
Direction
promotinge0
Value
85%e0
Comparator
81%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NaCl priming lowered the detrimental effect of salt stress on germination by promoting germination from 81% to 85% under salinity of 80 mM”

✓ located verbatim in the source · abstract, characters 391–529 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl priming promoted germination from 39% to 68% at 160 mM salinity.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Value
68%e0
Comparator
39%e0

Not reportedTreatmentOutcomeDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“from 39% to 68% under salinity of 160 mM”

✓ located verbatim in the source · abstract, characters 531–571 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl priming promoted germination from 15% to 56% at 240 mM salinity.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Outcome
maximum alleviatione0
Value
56%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“from 15% to 56% under salinity of 240 mM NaCl (maximum alleviation)”

✓ located verbatim in the source · abstract, characters 577–644 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl priming increased germination tolerance and seedling vigour indices.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
NaCl priminge0
Outcome
germination tolerance index and the vigour index of seedlingse0
Direction
increasede0
Comparator
control seedse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NaCl priming increased the germination tolerance index and the vigour index of seedlings as compared with the control seeds”

✓ located verbatim in the source · abstract, characters 659–782 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaf relative water content decreased with increasing salinity.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
increasing salinitye0
Outcome
Relative water content of the leavese0
Direction
decreasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Relative water content of the leaves was decreased with increasing salinity”

✓ located verbatim in the source · abstract, characters 784–859 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl priming lessened the salinity-related loss of relative water content.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
NaCl priminge0
Outcome
relative water contente0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NaCl priming reduced the decrease in relative water content under salinity”

✓ located verbatim in the source · abstract, characters 932–1006 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

At 240 mM NaCl, seedling dry weight fell 80.9% in non-primed seeds and 55.8% in primed seeds.

Taxon
rapeseede0
Outcome
dry weight of rapeseed seedlingse0
Direction
reducede0
Value
80.9%e0
Qualifier
55.8%e0

Verbatim

e0

“the salinity level of 240 mM NaCl reduced the dry weight of rapeseed seedlings by 80.9% in the non‐primed seeds and by 55.8% in the primed seeds”

✓ located verbatim in the source · abstract, characters 1034–1178 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 240 mM NaCl, priming improved the mean productivity index 1.26 times.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
NaCl priminge0
Outcome
mean productivity indexe0
Direction
improvede0
Value
1.26 timese0
Comparator
non‐primed seedse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NaCl priming improved the mean productivity index by 1.26 times under salinity of 240 mM NaCl and the harmonic mean index by 1.98 times under salinity of 240 mM NaCl compared with non‐primed seeds”

✓ located verbatim in the source · abstract, characters 1180–1376 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 240 mM NaCl, priming improved the harmonic mean index 1.98 times.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Treatment
NaCl priminge0
Outcome
harmonic mean indexe0
Direction
improvede0
Value
1.98 timese0
Comparator
non‐primed seedse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NaCl priming improved the mean productivity index by 1.26 times under salinity of 240 mM NaCl and the harmonic mean index by 1.98 times under salinity of 240 mM NaCl compared with non‐primed seeds”

✓ located verbatim in the source · abstract, characters 1180–1376 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend testing priming across relevant genotypes and saline conditions.

Taxon
Rapeseed (Brassica napus L.)Brassica napust0
Outcome
The priming method needs to be examined in a selection of relevant genotypes under a range of saline conditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The priming method needs to be examined in a selection of relevant genotypes under a range of saline conditions”

✓ located verbatim in the source · abstract, characters 1512–1623 · show in text ↑
t0

“Rapeseed (Brassica napus L.)”

✓ located verbatim in the source · title, characters 0–28 · show in text ↑

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.