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“dry weight decrease”

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2000 ’10 ’20

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Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Heat-treated transgenic stem extracts had reduced xylan molecular weight compared with wild type.

Taxon: plantt0
Organ / stage: dry stemse0
Treatment: transgenic extractse0
Outcome: molecular weight of xylanse0
Direction: decreasee0
Comparator: wildtypee0

Verbatim

e0
“Analysis of cell wall polysaccharides after heat treatment of wildtype and transgenic extracts from dry stems showed a decrease in the molecular weight of xylans from transgenic stems.”
✓ located verbatim in the source · abstract, characters 869–1053

t0
“plant xylans”
✓ located verbatim in the source · title, characters 18–30

Title

Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo‐xylanases

Bernhard Borkhardt; Jesper Harholt; Peter Ulvskov; et al. Plant Biotechnology Journal 2010. doi:10.1111/j.1467-7652.2010.00506.x

Other claims from this paper (6)

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Double mutant seeds had 35.2% less total RFO content, falling from 64.7 to 41.95 mg/g dry weight.

Taxon: soybeanGlycine maxe0
Material: double mutant linese0
Organ / stage: seede0
Treatment: double mutant linese0
Outcome: total RFO contente0
Direction: reductione0
Value: 35.2%e0

Not reportedComparator

Verbatim

e0
“The seed carbohydrate analysis of double mutant lines showed a reduction in the total RFO content of soybean seed from 64.7 mg/g dry weight to 41.95 mg/g dry weight, a 35.2% decrease”
✓ located verbatim in the source · abstract, characters 804–986

Title

CRISPR/Cas9-mediated knockout of galactinol synthase-encoding genes reduces raffinose family oligosaccharide levels in soybean seeds

Phat Do; Ha Chu; Huy Le; et al. Proceedings of The 1st International Electronic Conference on Plant Science 2020. doi:10.3390/iecps2020-08852

Other claims from this paper (9)

Method / resource The CRISPR construct induced deletions at target sites of both genes.
Observation Some induced alleles passed to progeny and T2 null segregants lacked induced off-target mutations.
Intervention effect Stachyose content declined by 35.4% in double mutant soybean.
Intervention effect Raffinose content rose by 41.7% in double mutant soybean.
Intervention effect Verbascose content decreased slightly in mutant lines.
Intervention effect Some mutant lines had increased seed protein and fat contents.
Intervention effect Mutants showed no observed differences in seed germination, development or morphology.
Mechanism The authors infer that GmGOLS1A and GmGOLS1B contribute to soybean oligosaccharide profiles through RFO biosynthesis.
Recommendation The authors present the genes as targets for crop improvement.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The noeA deletion mutant increased nodule fresh weight in five alfalfa cultivars.

Taxon: alfalfaMedicago sativae0
Material: Putong Zihua, Baoding, Ningxia, Baifa and Aohane0
Treatment: noeA deletion mutant 042BMA-Kme0
Outcome: fresh weight of nodulee0
Direction: increasee0
Comparator: 042BMe0

Verbatim

e0
“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
✓ located verbatim in the source · abstract, characters 401–655

Title

[Cloning, deletion and functional analysis of noeA from Sinorhizobium meliloti 042BM].

Bing-hai Du; Ju-quan Jiang; Xiao-hong Li; et al. PubMed 2005

Other claims from this paper (9)

Observation The 042BM noeA sequence was 99% identical to the S. meliloti 1021 sequence.
Observation NoeA similarity between 042BM and S. meliloti 1021 was 97%.
Observation NoeA shared 32% similarity with a Mesorhizobium methyltransferase.
Observation NoeA residues 303-362 had 41% similarity to an E. coli methyltransferase region.
Intervention effect The noeA deletion mutant increased nodule number in five alfalfa cultivars.
Intervention effect The noeA deletion mutant increased plant top dry weight in five alfalfa cultivars.
Intervention effect The noeA deletion mutant decreased the three measured traits on Milu alfalfa.
Intervention effect The mutant showed no significant change in nodulation of Huanghou and Zahua.
Mechanism The authors conclude that noeA contributes to cultivar-specific alfalfa nodulation.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The noeA deletion mutant increased nodule number in five alfalfa cultivars.

Taxon: alfalfaMedicago sativae0
Material: Putong Zihua, Baoding, Ningxia, Baifa and Aohane0
Treatment: noeA deletion mutant 042BMA-Kme0
Interactor: 042BMA-Kme0symbiont
Gene: noeAe0
Outcome: number of nodulee0
Direction: increasee0
Comparator: 042BMe0

Verbatim

e0
“Compared to 042BM, the noeA deletion mutant 042BMA-Km showed different degrees of increase in number of nodule, fresh weight of nodule and plant top dry weight on alfalfa cultivars of Putong Zihua, Baoding, Ningxia, Baifa and Aohan, but decrease on Milu.”
✓ located verbatim in the source · abstract, characters 401–655

Title

[Cloning, deletion and functional analysis of noeA from Sinorhizobium meliloti 042BM].

Bing-hai Du; Ju-quan Jiang; Xiao-hong Li; et al. PubMed 2005

Other claims from this paper (9)

Observation The 042BM noeA sequence was 99% identical to the S. meliloti 1021 sequence.
Observation NoeA similarity between 042BM and S. meliloti 1021 was 97%.
Observation NoeA shared 32% similarity with a Mesorhizobium methyltransferase.
Observation NoeA residues 303-362 had 41% similarity to an E. coli methyltransferase region.
Intervention effect The noeA deletion mutant increased nodule fresh weight in five alfalfa cultivars.
Intervention effect The noeA deletion mutant increased plant top dry weight in five alfalfa cultivars.
Intervention effect The noeA deletion mutant decreased the three measured traits on Milu alfalfa.
Intervention effect The mutant showed no significant change in nodulation of Huanghou and Zahua.
Mechanism The authors conclude that noeA contributes to cultivar-specific alfalfa nodulation.

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