Search claims · PlantClaims

Observationobservedoriginal resultmeasured

Claim · model-written summary

Six PAL genes encoding PAL proteins were identified in the Medicago truncatula genome.

Taxon: Medicago truncatulaMedicago truncatulae0
Gene: PALe0
Outcome: Six Medicago truncatula PAL genes that encode PAL proteinse0

Verbatim

e0
“Six Medicago truncatula PAL genes that encode PAL proteins were identified in the Medicago truncatula genome”
✓ located verbatim in the source · abstract, characters 654–762

Title

Genome-wide identification and characterization of the Phenylalanine Ammonia-lyase (PAL) gene family in Medicago truncatula

Wei Ren; Yingzhe Wang; Ankai Xu; et al. Legume Research - An International Journal 2019. doi:10.18805/lr-431

Other claims from this paper (3)

Observationobservedoriginal resultmeasured

Claim · model-written summary

PAL-1 cDNA expression in E. coli produced catalytically active PAL.

Taxon: parsleyPetroselinum crispume1
Gene: PAL-1e0
Outcome: catalytically active PALe0

Verbatim

e0
“Transformation of Escherichia coli cells with PAL-1 or PAL-4 cDNA yielded catalytically active PAL”
✓ located verbatim in the source · abstract, characters 337–435

e1
“In parsley (Petroselinum crispum)”
✓ located verbatim in the source · abstract, characters 0–33

Title

Expression in Escherichia coli of catalytically active phenylalanine ammonia‐lyase from parsley

Wolfgang Schulz; Hans-Georg Eiben; Klaus Hahlbrock FEBS Letters 1989. doi:10.1016/0014-5793(89)81687-4

Other claims from this paper (4)

Observation Parsley PAL is encoded by four structurally similar genes.
Method / resource The near full-length PAL-4 cDNA and protein sequence were presented and compared with PAL-1.
Observation PAL-4 cDNA expression in E. coli produced catalytically active PAL.
Mechanism PAL catalytic center formation appears spontaneous rather than plant-specific.

Observationobservedoriginal resultmeasured

Claim · model-written summary

PAL-4 cDNA expression in E. coli produced catalytically active PAL.

Taxon: parsleyPetroselinum crispume1
Gene: PAL-4e0
Outcome: catalytically active PALe0

Verbatim

e0
“Transformation of Escherichia coli cells with PAL-1 or PAL-4 cDNA yielded catalytically active PAL”
✓ located verbatim in the source · abstract, characters 337–435

e1
“In parsley (Petroselinum crispum)”
✓ located verbatim in the source · abstract, characters 0–33

Title

Expression in Escherichia coli of catalytically active phenylalanine ammonia‐lyase from parsley

Wolfgang Schulz; Hans-Georg Eiben; Klaus Hahlbrock FEBS Letters 1989. doi:10.1016/0014-5793(89)81687-4

Other claims from this paper (4)

Observation Parsley PAL is encoded by four structurally similar genes.
Method / resource The near full-length PAL-4 cDNA and protein sequence were presented and compared with PAL-1.
Observation PAL-1 cDNA expression in E. coli produced catalytically active PAL.
Mechanism PAL catalytic center formation appears spontaneous rather than plant-specific.

Observationobservedoriginal resultmeasured

Claim · model-written summary

EsPAL and Pinus PAL formed a gymnosperm-type PAL subfamily.

Taxon: Ephedra sinicaEphedra sinicat0
Gene: EsPALe0
Outcome: gymnosperm-type PAL subfamilye0

Verbatim

e0
“EsPAL and Pinus PAL formed a gymnosperm-type PAL subfamily”
✓ located verbatim in the source · abstract, characters 544–602

t0
“Ephedra sinica”
✓ located verbatim in the source · title, characters 67–81

Title

Molecular Characterization of the Phenylalanine Ammonia-Lyase from Ephedra sinica

Taketo Okada; Masayuki Mikage; Setsuko Sekita Biological and Pharmaceutical Bulletin 2008. doi:10.1248/bpb.31.2194

Other claims from this paper (6)

Observation The Espal open reading frame was 2166 bp and encoded 722 amino acids.
Observation Sequence analyses suggested at least four EsPAL isoforms with nine amino acid substitutions.
Observation Recombinant EsPAL1 to 4 catalyzed a PAL reaction.
Observation The four recombinant isoforms did not differ significantly in their reported kinetic values.
Observation Espal gene expression was higher in roots than aerial parts.
Observation PAL activity was higher in roots than aerial parts.

Observationobservedoriginal resultmeasured

Claim · model-written summary

PAL-1 and PAL-2 promoters and known PAL proteins showed extensive structural similarity.

Gene: PAL-1 and PAL-2e0
Outcome: Extensive structural similaritiese0

Not reportedTaxonOrgan / stage

Verbatim

e0
“Extensive structural similarities were observed both between the TATA-proximal portions of the PAL-1 and PAL-2 promoters, particularly in the areas containing putative cis-acting elements, and among all presently known PAL proteins from various higher and lower plants.”
✓ located verbatim in the source · abstract, characters 595–864

Title

Phenylalanine ammonia‐lyase in potato (Solanum tuberosum L.)

Hans‐Jürgen JOOS; Klaus HAHLBROCK European Journal of Biochemistry 1992. doi:10.1111/j.1432-1033.1992.tb16675.x

Other claims from this paper (7)

Observationobservedoriginal resultmeasured

Claim · model-written summary

PAL transgenic hairy roots differed in PAL activity.

Taxon: chilli pepper (Capsicum frutescens)Capsicum frutescense1
Gene: PAL transgenee0
Outcome: PAL activitye0
Direction: differente0

Verbatim

e0
“Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.”
✓ located verbatim in the source · abstract, characters 792–899

e1
“chilli pepper (Capsicum frutescens)”
✓ located verbatim in the source · abstract, characters 22–57

Title

Transformation of Chilli Pepper (Capsicum frutescens) with a Phenylalanine Ammonia-Lyase Gene

Takashi YAMAKAWA; Susumu SEKIGUCHI; Tohru KODAMA; et al. Plant Biotechnology 1998. doi:10.5511/plantbiotechnology.15.189

Other claims from this paper (4)

Observation GUS staining was predominant in vascular and root tip tissues.
Method / resource Southern hybridisation confirmed transformation of the hairy roots.
Observation PAL transgenic hairy roots grew slowly.
Observation PAL transgenic hairy roots had altered morphology.

Observationobservedoriginal resultmeasured

Claim · model-written summary

CP-PAL is very closely related to PAL of Cucumis sativus in phylogeny.

Taxon: Cucurbita pepoCucurbita pepot0
Gene: CP-PALe6
Outcome: very closely relatede6
Comparator: PAL of C. sativuse6

Verbatim

t0
“Cucurbita pepo”
✓ located verbatim in the source · title, characters 60–74

e6
“Phylogenetic tree analysis indicated that CP-PAL was very closely related to PAL of C. sativus”
✓ located verbatim in the source · abstract, characters 2352–2446

Title

Cloning and Expression Analysis of PAL Gene in Seed Coat of Cucurbita pepo

Liu Ji Zhongguo nongye Kexue 2014

Other claims from this paper (9)

Observationobservedoriginal resultmeasured

Claim · model-written summary

The deduced PAL sequence showed 89% identity with rice PAL.

Taxon: plantainMusa ABB Groupe0
Gene: PALe1
Outcome: identity with rice PALe1
Value: 89%e1

Verbatim

e0
“plantain (Musa ABB cv. Dongguandajiao)”
✓ located verbatim in the source · abstract, characters 58–96

e1
“deduced amino acid sequence had 89% identity with rice PAL”
✓ located verbatim in the source · abstract, characters 246–304

Title

Cloning, Characterization and Expression of a Phenylalanine Ammonialyase Gene (M-PAL) from Plantain (Musa ABB cv. Dongguandajiao)

Xue‐Lin Huang Redai yaredai zhiwu xuebao 2007

Other claims from this paper (8)

Method / resource A full-length PAL cDNA was cloned from plantain leaves.
Observation The PAL cDNA contained a 1 191 bp complete ORF.
Observation The PAL ORF encoded a 397 amino-acid protein.
Observation Plantain has a small family of four to five PAL genes.
Observation Recombinant plantain PAL protein was about 43 KD and matched its predicted molecular weight.
Observation The recombinant plantain protein showed phenylalanine ammonia-lyase activity.
Intervention effect M-PAL transcript increased after plantain inoculation with Fusarium oxysporum f. sp. cubense.
Association The authors suggest M-PAL expression may relate to Fusarium wilt resistance.

Observationobservedoriginal resultmeasured

Claim · model-written summary

CP-PAL contains three functional domains and the typical PAL active site sequence.

Taxon: Cucurbita pepoCucurbita pepot0
Gene: CP-PALe4
Outcome: functional domainse4
Value: threee4

Verbatim

t0
“Cucurbita pepo”
✓ located verbatim in the source · title, characters 60–74

e4
“CP-PAL contained three functional domains of PAL-HAL, PLN02457, phe_am_lyase and the typical PAL enzyme active site sequence(GTITASGDLVPLSYIA)”
✓ located verbatim in the source · abstract, characters 1191–1333

Title

Cloning and Expression Analysis of PAL Gene in Seed Coat of Cucurbita pepo

Liu Ji Zhongguo nongye Kexue 2014

Other claims from this paper (9)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Alfalfa and poplar PAL antibodies recognized plant PAL strongly but fungal PAL weakly.

Taxon: AlfalfaMedicago sativae0
Gene: PALe0
Outcome: strongly recognized plant PALs but only weakly recognized fungal PALse0

Verbatim

e0
“Both anti-Alfalfa and anti-popular PAL antibodies strongly recognized plant PALs but only weakly recognized fungal PALs.”
✓ located verbatim in the source · abstract, characters 1088–1208

Title

Immunological Relationships among Fungal and Plant Phenylalanine Ammonia-lyases and Bacterial Histidine Ammonia-lyase

Min-Woo Hyun; Yeo-Hong Yun; Dong-Yeon Suh; et al. The Korean Journal of Mycology 2011. doi:10.4489/kjm.2010.39.3.205

Other claims from this paper (3)

“PAL”

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