Search claims · PlantClaims

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

The extent of H2O2 staining increased significantly with H2O2 stress.

Taxon: Grass Peat0
Treatment: H2O2e0
Outcome: scope and extent of H2O2 staininge0
Direction: increasede0

Not reportedComparator

Verbatim

e0
“The scope and extent of H2O2 staining was significantly increased”
✓ located verbatim in the source · abstract, characters 601–666

t0
“Grass Pea”
✓ located verbatim in the source · title, characters 27–36

Title

Physiological Responses of Grass Pea Root to Oxidative Stress Induced by H_2O_2

Jiang Jinglon Xibei zhiwu xuebao 2014

Other claims from this paper (11)

Intervention effect Fresh root weight decreased significantly as H2O2 concentration increased.
Intervention effect Lateral root number did not change significantly as H2O2 concentration increased.
Observation H2O2 levels remained relatively low in the root apical region.
Intervention effect The extent of O-·2 staining decreased significantly.
Observation The apical region maintained a high level of O-·2.
Intervention effect Ascorbic acid content rose initially and then declined after stress.
Intervention effect Catalase, peroxidase and ascorbate peroxidase activities rose initially and then declined.
Intervention effect Superoxide dismutase showed an upward trend.
Association O-·2 accumulation was positively related to root growth and vigor.
Association H2O2 accumulation was positively related to oxidative stress extent.
Intervention effect Low H2O2 concentrations improved antioxidant scavenging ability for ROS in roots.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Coronatine intensified DAB and NBT staining of H2O2 and O2•-.

Taxon: cottonGossypium hirsutumt0
Treatment: CORe1
Outcome: DAB staining of H2O2 and the NBT staining of O2•-e1
Direction: intensifiede1

Not reportedComparator

Verbatim

t0
“Cotton Roots”
✓ located verbatim in the source · title, characters 40–52

e1
“Additionally, COR intensified the DAB staining of H2O2 and the NBT staining of O2•-”
✓ located verbatim in the source · abstract, characters 1117–1200

Title

Lack of K-Dependent Oxidative Stress in Cotton Roots Following Coronatine-Induced ROS Accumulation

Zhiyong Zhang; Xin Zhang; Zebing Hu; et al. PLoS ONE 2015. doi:10.1371/journal.pone.0126476

Other claims from this paper (9)

Intervention effect Coronatine increased root vitality of cotton seedlings under K-replete and K-deficient conditions, by 2.7- and 3.5-fold.
Intervention effect Coronatine significantly decreased lipid peroxidation in cotton seedlings.
Intervention effect Coronatine significantly increased SOD activity under K-replete conditions only.
Intervention effect Coronatine significantly increased CAT, GPX and APX activities under K-replete and K-deficient conditions.
Intervention effect Coronatine significantly increased DPPH-radical scavenging activity.
Intervention effect Coronatine increased superoxide anion concentrations by 1.6- and 1.7-fold.
Intervention effect Coronatine increased hydrogen peroxide levels by 5.7- and 2.1-fold.
Intervention effect Coronatine-induced ROS accumulation did not cause oxidative stress in cotton roots.
Intervention effect Coronatine stimulated the activities of most antioxidant enzymes in cotton roots.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

No staining was detectable without added H2O2.

Taxon: wintering poplare1
Treatment: absence of added H2O2e0
Outcome: staininge0
Direction: no detectablee0

Not reportedComparator

Verbatim

e0
“There was no detectable staining in the absence of added H2O2”
✓ located verbatim in the source · abstract, characters 314–375

e1
“wintering poplar”
✓ located verbatim in the source · abstract, characters 162–178

Title

Cytochemical Evidence for the Occurrence in Plants of a Novel Microbody that Contains Peroxidase

Shonosuke Sagisaka; Minoru Asada Plant and Cell Physiology 1986. doi:10.1093/oxfordjournals.pcp.a077262

Other claims from this paper (3)

Observation Peroxidase was detected in wintering poplar xylem parenchyma microbodies.
Intervention effect 3-amino-l,2,4-triazole did not inhibit the peroxidative reaction.
Intervention effect KCN completely inhibited the peroxidative reaction.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Heat-treated jac1 mutants showed stronger DAB staining, consistent with higher apparent H2O2 levels.

Taxon: Arabidopsis thalianaArabidopsis thalianae0
Material: jac1 mutantse3
Treatment: heate3
Outcome: DAB staininge3
Direction: strongere3
Qualifier: consistent with higher apparent H2O2 levelse3

Not reportedComparator

Verbatim

e0
“as a positive regulator of thermotolerance in Arabidopsis thaliana (A. thaliana)”
✓ located verbatim in the source · abstract, characters 550–630

e3
“heat-treated jac1 mutants showed stronger DAB staining, consistent with higher apparent H2O2 levels, whereas JAC1 overexpression lines showed weaker staining”
✓ located verbatim in the source · abstract, characters 939–1096

Title

JAC1 Promotes Thermotolerance in Arabidopsis by Limiting Heat-Induced H2O2 Accumulation and Protecting PGLP1 from Sulfenylation-Mediated Inhibition

Binglei Zhang; Ruichao Li; Ke Li; et al. Plants 2026. doi:10.3390/plants15050699

Other claims from this paper (14)

Observation JAC1 transcripts were rapidly induced by heat treatment.
Intervention effect jac1 mutants were hypersensitive to long-term heat stress.
Intervention effect JAC1 overexpression lines exhibited enhanced survival under long-term heat stress.
Intervention effect Heat-treated JAC1 overexpression lines showed weaker DAB staining than the other genotypes.
Observation PGLP1 abundance (mRNA and protein) was unchanged among genotypes.
Intervention effect Heat caused a marked reduction in leaf PGLP1 enzymatic activity in jac1 mutants.
Intervention effect Heat led to a significant increase in leaf PGLP1 enzymatic activity in JAC1 overexpression lines.
Association Heat changed 2PG content correspondingly.
Observation PGLP1 sulfenylation was detectable only after heat.
Association PGLP1 sulfenylation was enhanced in jac1 mutants.
Association PGLP1 sulfenylation was suppressed in JAC1 overexpression lines.
Intervention effect Overexpressing PGLP1 in jac1-1 rescued heat sensitivity.
Intervention effect Knocking down PGLP1 in the JAC1 overexpression background reversed thermotolerance.
Mechanism JAC1 maintains heat-induced H2O2 homeostasis, which limits PGLP1 sulfenylation and sustains PGLP1 activity.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Aminoguanidine suppressed DAB staining and H2O2 fluorescence in mung bean hypocotyls.

Taxon: mung beanVigna radiatae0
Treatment: AGe1
Outcome: DAB staining and H2O2 fluorescencee1
Direction: suppressede1

Not reportedComparator

Verbatim

e0
“The role of hydrogen peroxide (H2O2) generated by copper-containing diamine oxidases (CuAO) in adventitious rooting in mung bean hypocotyl was studied”
✓ located verbatim in the source · abstract, characters 0–150

e1
“AG largely suppressed DAB staining and H2O2 fluorescence, and prevented root primordium formation”
✓ located verbatim in the source · abstract, characters 1260–1357

Title

Hydrogen peroxide generated by copper amine oxidase involved in adventitious root formation in mung bean hypocotyl cuttings

Xiao-Ping She; Ai-Xia Huang; Yun Ren Australian Journal of Botany 2010. doi:10.1071/bt10187

Other claims from this paper (7)

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

H2O2 accumulated in the older leaves under K+ deficiency treatment, shown by strong diaminobenzidine staining.

Taxon: Neolamarckia cadambaNeolamarckia cadambat0
Organ / stage: older leavese5
Treatment: K+ deficiency treatmente5
Outcome: accumulation of H2O2e5

Verbatim

t0
“Neolamarckia cadamba (Roxb.) Bosser”
✓ located verbatim in the source · title, characters 78–113

e5
“Strong diaminobenzidine staining was observed on the older leaves showing accumulation of H2O2 during K+ deficiency treatment”
✓ located verbatim in the source · abstract, characters 889–1014

Title

Potassium deficiency inhibits leaf growth and promotes leaf necrotic spots in Neolamarckia cadamba (Roxb.) Bosser

Yi Liu; Lu Lu; Yuanyuan Zhang; et al. Tree Physiology 2021. doi:10.1093/treephys/tpab172

Other claims from this paper (8)

Intervention effect K+ deficiency gave smaller leaves in Neolamarckia cadamba.
Intervention effect K+ deficiency caused necrotic spots on the older leaves.
Intervention effect K+ deficiency downregulated gene modules for cell proliferation, cell cycle and cell expansion.
Intervention effect K+ deficiency increased the activity of ROS scavenging enzymes, superoxide dismutase and ascorbate peroxidases.
Intervention effect K+ deficiency increased malondialdehyde in Neolamarckia cadamba.
Intervention effect Expression of DEGs related to ROS scavenging enzymes was upregulated under K+ deficiency.
Association Putrescine and ethylene synthesis genes were upregulated under K+ deficiency.
Observation Fifteen DEGs in response to ethylene signaling were upregulated in the third week.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Compared with the control group, a high level of H2O2 was detected by DAB staining in the leaf vein of tobacco under UV-B stress.

Taxon: Nicotiana tabacum L.Nicotiana tabacumt0
Organ / stage: vein of the leavee0
Treatment: UV- B stresse0
Outcome: H2O2e0
Direction: high levele0
Comparator: control groupe0

Verbatim

t0
“Nicotiana tabacum L.”
✓ located verbatim in the source · title, characters 69–89

e0
“Compared with the control group,a high level of H2O2 was detected by DAB staining in the vein of the leave under UV- B stress”
✓ located verbatim in the source · abstract, characters 346–471

Title

Effects of Different Plant Growth Regulators on Stress Resistance of Nicotiana tabacum L. Seedling under UV- B Illumination

Ji Wang Journal of Chuxiong Normal University 2014

Other claims from this paper (2)

Intervention effect Exogenous phytohormones enhanced the resistance of tobacco seedlings under UV-B radiation to a certain extent.
Intervention effect GA and the 6-BA + IAA double treatments had the most obvious effects on resistance of tobacco seedlings under UV-B.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Catalase treatment almost eliminated hydrogen peroxide staining.

Taxon: lettuceLactuca sativat0
Treatment: Catalase treatmente0
Gene: Catalasee0
Outcome: staininge0
Direction: eliminatede0

Not reportedComparator

Verbatim

e0
“Catalase treatment to decompose H2O2 almost entirely eliminated staining”
✓ located verbatim in the source · abstract, characters 931–1003

t0
“lettuce cells”
✓ located verbatim in the source · title, characters 85–98

Title

Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola.

C S Bestwick; I R Brown; M H Bennett; et al. The Plant Cell 1997. doi:10.1105/tpc.9.2.209

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Heat stress produced intense DAB staining across finger millet genotypes.

Taxon: Finger MilletEleusine coracanat0
Treatment: High temperature stresse0
Outcome: intense DAB staininge0

Not reportedComparatorDirection

Verbatim

e0
“High temperature stress caused intense DAB staining in all genotypes, indicating severe oxidative stress, with GPU 67 showing the highest H₂O₂ accumulation and GPU 28 the lowest.”
✓ located verbatim in the source · abstract, characters 1208–1386

t0
“Finger Millet”
✓ located verbatim in the source · title, characters 62–75

Title

Balancing of Oxidative Stress and Antioxidant Accumulation in Finger Millet under High Temperature and Elevated CO₂ × Drought Stress Interactions

Anusree K.; Manju R.V.; Soni K.B.; et al. International Journal of Environment and Climate Change 2025. doi:10.9734/ijecc/2025/v15i115113

Other claims from this paper (10)

Observation GPU 67 had the highest H₂O₂ accumulation under heat stress.
Observation GPU 28 had the lowest H₂O₂ accumulation under heat stress.
Intervention effect Elevated CO₂ with drought produced lighter DAB staining.
Mechanism The authors interpreted the lighter staining as reduced ROS buildup from CO₂ mitigation.
Intervention effect Antioxidant activity increased under both stress conditions.
Intervention effect ML 365 showed a fivefold antioxidant activity increase under heat stress.
Intervention effect GPU 28 showed a fourfold antioxidant activity increase under heat stress.
Intervention effect Elevated CO₂ and drought enhanced antioxidant levels to a lesser degree.
Intervention effect High temperature imposed the greatest oxidative load on finger millet.
Intervention effect Elevated CO₂ alleviated stress effects on finger millet.

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

GPU 67 had the highest H₂O₂ accumulation under heat stress.

Taxon: Finger MilletEleusine coracanat0
Material: GPU 67e0
Outcome: H₂O₂ accumulatione0
Direction: higheste0

Verbatim

e0
“High temperature stress caused intense DAB staining in all genotypes, indicating severe oxidative stress, with GPU 67 showing the highest H₂O₂ accumulation and GPU 28 the lowest.”
✓ located verbatim in the source · abstract, characters 1208–1386

t0
“Finger Millet”
✓ located verbatim in the source · title, characters 62–75

Title

Balancing of Oxidative Stress and Antioxidant Accumulation in Finger Millet under High Temperature and Elevated CO₂ × Drought Stress Interactions

Anusree K.; Manju R.V.; Soni K.B.; et al. International Journal of Environment and Climate Change 2025. doi:10.9734/ijecc/2025/v15i115113

Other claims from this paper (10)

Intervention effect Heat stress produced intense DAB staining across finger millet genotypes.
Observation GPU 28 had the lowest H₂O₂ accumulation under heat stress.
Intervention effect Elevated CO₂ with drought produced lighter DAB staining.
Mechanism The authors interpreted the lighter staining as reduced ROS buildup from CO₂ mitigation.
Intervention effect Antioxidant activity increased under both stress conditions.
Intervention effect ML 365 showed a fivefold antioxidant activity increase under heat stress.
Intervention effect GPU 28 showed a fourfold antioxidant activity increase under heat stress.
Intervention effect Elevated CO₂ and drought enhanced antioxidant levels to a lesser degree.
Intervention effect High temperature imposed the greatest oxidative load on finger millet.
Intervention effect Elevated CO₂ alleviated stress effects on finger millet.

“staining of H2O2”

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