By interacting organism › Bacteria › Phyllobacteriaceae › Mesorhizobium loti

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper reduced Lotus japonicus shoot biomass by 30-40% relative to control in copper-exposed nodulated plants

Taxon: Lotus japonicusLotus japonicuse1
Organ / stage: shootse2
Treatment: 300 or 450 µM of coppere1
Interactor: Mesorhizobium lotiMesorhizobium lotie1symbiont
Outcome: biomasse2
Direction: reducede2
Value: 30-40%e2
Comparator: controle2

Verbatim

e1
“When Lotus japonicus inoculated with its rhizobial partner Mesorhizobium loti was exposed to 300 or 450 µM of copper”
✓ located verbatim in the source · abstract, characters 384–500

e2
“biomass was reduced by 30-40% in the shoots, 40-55% in the roots, and 40-60% in the nodules relative to control”
✓ located verbatim in the source · abstract, characters 502–613

Title

Copper causes reduced nitrogen fixation but does not accumulate in the nodules of the legume Lotus japonicus

Kathryn Lamoureux; Sheila M. Macfie PLoS ONE 2026. doi:10.1371/journal.pone.0349086

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper reduced Lotus japonicus root biomass by 40-55% relative to control in copper-exposed nodulated plants

Taxon: Lotus japonicusLotus japonicuse1
Organ / stage: rootse2
Treatment: 300 or 450 µM of coppere1
Interactor: Mesorhizobium lotiMesorhizobium lotie1symbiont
Outcome: biomasse2
Direction: reducede2
Value: 40-55%e2
Comparator: controle2

Verbatim

e1
“When Lotus japonicus inoculated with its rhizobial partner Mesorhizobium loti was exposed to 300 or 450 µM of copper”
✓ located verbatim in the source · abstract, characters 384–500

e2
“biomass was reduced by 30-40% in the shoots, 40-55% in the roots, and 40-60% in the nodules relative to control”
✓ located verbatim in the source · abstract, characters 502–613

Title

Copper causes reduced nitrogen fixation but does not accumulate in the nodules of the legume Lotus japonicus

Kathryn Lamoureux; Sheila M. Macfie PLoS ONE 2026. doi:10.1371/journal.pone.0349086

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper reduced Lotus japonicus nodule biomass by 40-60% relative to control

Taxon: Lotus japonicusLotus japonicuse1
Organ / stage: nodulese2
Treatment: 300 or 450 µM of coppere1
Interactor: Mesorhizobium lotiMesorhizobium lotie1symbiont
Outcome: biomasse2
Direction: reducede2
Value: 40-60%e2
Comparator: controle2

Verbatim

e1
“When Lotus japonicus inoculated with its rhizobial partner Mesorhizobium loti was exposed to 300 or 450 µM of copper”
✓ located verbatim in the source · abstract, characters 384–500

e2
“biomass was reduced by 30-40% in the shoots, 40-55% in the roots, and 40-60% in the nodules relative to control”
✓ located verbatim in the source · abstract, characters 502–613

Title

Copper causes reduced nitrogen fixation but does not accumulate in the nodules of the legume Lotus japonicus

Kathryn Lamoureux; Sheila M. Macfie PLoS ONE 2026. doi:10.1371/journal.pone.0349086

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper exposure decreased nitrogen fixation in Lotus japonicus by 40-45%

Taxon: Lotus japonicusLotus japonicuse1
Treatment: 300 or 450 µM of coppere1
Interactor: Mesorhizobium lotiMesorhizobium lotie1symbiont
Outcome: nitrogen fixatione6
Direction: decreasede6
Value: 40-45%e6
Qualifier: which can be indicators of early nodule senescencee6

Not reportedComparator

Verbatim

e1
“When Lotus japonicus inoculated with its rhizobial partner Mesorhizobium loti was exposed to 300 or 450 µM of copper”
✓ located verbatim in the source · abstract, characters 384–500

e6
“nitrogen fixation and ascorbate peroxidase activity decreased by 40-45% and 40-60%, respectively, which can be indicators of early nodule senescence”
✓ located verbatim in the source · abstract, characters 934–1082

Title

Copper causes reduced nitrogen fixation but does not accumulate in the nodules of the legume Lotus japonicus

Kathryn Lamoureux; Sheila M. Macfie PLoS ONE 2026. doi:10.1371/journal.pone.0349086

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper exposure decreased ascorbate peroxidase activity in Lotus japonicus nodules by 40-60%

Taxon: Lotus japonicusLotus japonicuse1
Treatment: 300 or 450 µM of coppere1
Interactor: Mesorhizobium lotiMesorhizobium lotie1symbiont
Outcome: ascorbate peroxidase activitye6
Direction: decreasede6
Value: 40-60%e6

Not reportedComparator

Verbatim

e1
“When Lotus japonicus inoculated with its rhizobial partner Mesorhizobium loti was exposed to 300 or 450 µM of copper”
✓ located verbatim in the source · abstract, characters 384–500

e6
“nitrogen fixation and ascorbate peroxidase activity decreased by 40-45% and 40-60%, respectively, which can be indicators of early nodule senescence”
✓ located verbatim in the source · abstract, characters 934–1082

Title

Copper causes reduced nitrogen fixation but does not accumulate in the nodules of the legume Lotus japonicus

Kathryn Lamoureux; Sheila M. Macfie PLoS ONE 2026. doi:10.1371/journal.pone.0349086

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Albendazole reduced root infection threads.

Taxon: Lotus japonicusLotus japonicust0
Treatment: Albendazolee0
Interactor: Mesorhizobium lotiMesorhizobium lotit0symbiont
Outcome: number of infection threads formed in rootse0
Direction: reducede0

Not reportedComparator

Verbatim

e0
“Albendazole reduced the number of infection threads formed in roots”
✓ located verbatim in the source · abstract, characters 907–974

t0
“Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus”
✓ located verbatim in the source · title, characters 0–179

e1
“Albendazole reduced the number of infection threads formed in roots and downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 907–1020

Title

Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus

Polyxeni Gorgia; Stathis Lagos; Christina N. Nikolaou; et al. Environmental Toxicology 2026. doi:10.1002/tox.70101

Other claims from this paper (7)

Intervention effect Neither compound was phytotoxic to Lotus japonicus.
Intervention effect Albendazole inhibited rhizobial growth at 0.75 mg kg −1.
Intervention effect Fenbendazole inhibited rhizobial growth at 2.25 mg kg −1.
Intervention effect Albendazole downregulated symbiosis genes.
Intervention effect Fenbendazole showed no early symbiosis effects.
Intervention effect Albendazole did not significantly affect nodulation at advanced symbiosis stages.
Intervention effect Albendazole did not significantly affect nitrogenase activity at advanced symbiosis stages.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Albendazole downregulated symbiosis genes.

Taxon: Lotus japonicusLotus japonicust0
Treatment: Albendazolee1
Interactor: Mesorhizobium lotiMesorhizobium lotit0symbiont
Outcome: key symbiosis‐related genese0
Direction: downregulatede0

Not reportedComparator

Verbatim

e0
“downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 979–1020

t0
“Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus”
✓ located verbatim in the source · title, characters 0–179

e1
“Albendazole reduced the number of infection threads formed in roots and downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 907–1020

Title

Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus

Polyxeni Gorgia; Stathis Lagos; Christina N. Nikolaou; et al. Environmental Toxicology 2026. doi:10.1002/tox.70101

Other claims from this paper (7)

Intervention effect Neither compound was phytotoxic to Lotus japonicus.
Intervention effect Albendazole inhibited rhizobial growth at 0.75 mg kg −1.
Intervention effect Fenbendazole inhibited rhizobial growth at 2.25 mg kg −1.
Intervention effect Albendazole reduced root infection threads.
Intervention effect Fenbendazole showed no early symbiosis effects.
Intervention effect Albendazole did not significantly affect nodulation at advanced symbiosis stages.
Intervention effect Albendazole did not significantly affect nitrogenase activity at advanced symbiosis stages.

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fenbendazole showed no early symbiosis effects.

Taxon: Lotus japonicusLotus japonicust0
Treatment: fenbendazolee0
Interactor: Mesorhizobium lotiMesorhizobium lotit0symbiont
Outcome: no effectse0

Not reportedComparatorDirection

Verbatim

e0
“unlike fenbendazole, which showed no effects”
✓ located verbatim in the source · abstract, characters 1074–1118

t0
“Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus”
✓ located verbatim in the source · title, characters 0–179

e1
“Albendazole reduced the number of infection threads formed in roots and downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 907–1020

Title

Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus

Polyxeni Gorgia; Stathis Lagos; Christina N. Nikolaou; et al. Environmental Toxicology 2026. doi:10.1002/tox.70101

Other claims from this paper (7)

Intervention effect Neither compound was phytotoxic to Lotus japonicus.
Intervention effect Albendazole inhibited rhizobial growth at 0.75 mg kg −1.
Intervention effect Fenbendazole inhibited rhizobial growth at 2.25 mg kg −1.
Intervention effect Albendazole reduced root infection threads.
Intervention effect Albendazole downregulated symbiosis genes.
Intervention effect Albendazole did not significantly affect nodulation at advanced symbiosis stages.
Intervention effect Albendazole did not significantly affect nitrogenase activity at advanced symbiosis stages.

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Albendazole did not significantly affect nodulation at advanced symbiosis stages.

Taxon: Lotus japonicusLotus japonicust0
Treatment: albendazolee0
Interactor: Mesorhizobium lotiMesorhizobium lotit0symbiont
Outcome: nodulatione0
Direction: not significantly affectede0

Not reportedComparator

Verbatim

e0
“nodulation and nitrogenase activity were not significantly affected by albendazole at advanced stages of the symbiosis”
✓ located verbatim in the source · abstract, characters 1129–1247

t0
“Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus”
✓ located verbatim in the source · title, characters 0–179

e1
“Albendazole reduced the number of infection threads formed in roots and downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 907–1020

Title

Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus

Polyxeni Gorgia; Stathis Lagos; Christina N. Nikolaou; et al. Environmental Toxicology 2026. doi:10.1002/tox.70101

Other claims from this paper (7)

Intervention effect Neither compound was phytotoxic to Lotus japonicus.
Intervention effect Albendazole inhibited rhizobial growth at 0.75 mg kg −1.
Intervention effect Fenbendazole inhibited rhizobial growth at 2.25 mg kg −1.
Intervention effect Albendazole reduced root infection threads.
Intervention effect Albendazole downregulated symbiosis genes.
Intervention effect Fenbendazole showed no early symbiosis effects.
Intervention effect Albendazole did not significantly affect nitrogenase activity at advanced symbiosis stages.

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Albendazole did not significantly affect nitrogenase activity at advanced symbiosis stages.

Taxon: Lotus japonicusLotus japonicust0
Treatment: albendazolee0
Interactor: Mesorhizobium lotiMesorhizobium lotit0symbiont
Outcome: nitrogenase activitye0
Direction: not significantly affectede0

Not reportedComparator

Verbatim

e0
“nodulation and nitrogenase activity were not significantly affected by albendazole at advanced stages of the symbiosis”
✓ located verbatim in the source · abstract, characters 1129–1247

t0
“Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus”
✓ located verbatim in the source · title, characters 0–179

e1
“Albendazole reduced the number of infection threads formed in roots and downregulated key symbiosis‐related genes”
✓ located verbatim in the source · abstract, characters 907–1020

Title

Benzimidazole Anthelmintic Compounds Albendazole and Fenbendazole Show Distinct Toxicity on the Nitrogen Fixing Bacterium Mesorhizobium loti and Its Symbiosis With Lotus japonicus

Polyxeni Gorgia; Stathis Lagos; Christina N. Nikolaou; et al. Environmental Toxicology 2026. doi:10.1002/tox.70101

Other claims from this paper (7)

Intervention effect Neither compound was phytotoxic to Lotus japonicus.
Intervention effect Albendazole inhibited rhizobial growth at 0.75 mg kg −1.
Intervention effect Fenbendazole inhibited rhizobial growth at 2.25 mg kg −1.
Intervention effect Albendazole reduced root infection threads.
Intervention effect Albendazole downregulated symbiosis genes.
Intervention effect Fenbendazole showed no early symbiosis effects.
Intervention effect Albendazole did not significantly affect nodulation at advanced symbiosis stages.

Mesorhizobium loti 447 claims

447 claims