Claim · model-written summary
The apparent Km for i(6)Ade in Actinidia chinensis x Actinidia arguta callus is 12 muM.
- Taxon
- Actinidia chinensis x Actinidia argutaActinidia chinensis ×Actinidia argutae4
- Organ / stage
- calluse4
- Outcome
- apparent K(m) for i(6)Adee4
- Value
- 12 muMe4
Verbatim
e4“The apparent K(m) for i(6)Ade in A. kolomikta and Actinidia chinensis x Actinidia arguta callus and in tissue slices of A. arguta stems was 12 muM”
Title
Zeatin biosynthesis from N 6 -(Δ 2 -isopentenyl)adenine in Actinidia and other woody plants
John W. Einset Proceedings of the National Academy of Sciences 1986. doi:10.1073/pnas.83.4.972
Other claims from this paper (11)
- Observation Actively growing Actinidia kolomikta callus contained 2 and 0.5 nmol of cytokinins zeatin and i(6)Ade per gram fresh weight.
- Intervention effect Feeding i(6)Ade to cytokinin-free Actinidia kolomikta tissue increased endogenous io(6)Ade linearly to 160 nmol/g in 8 hr.
- Intervention effect Feeding i(6)Ade increased i(6)Ade content to 5 nmol/g in 2 hr, after which it declined.
- Observation The apparent Km for i(6)Ade in Actinidia kolomikta callus is 12 muM.
- Observation The apparent Km for i(6)Ade in Arguta tissue slices from stems is 12 muM.
- Observation The conversion of i(6)Ade to io(6)Ade in Actinidia kolomikta is O2-requiring and specific for i(6)Ade versus i(6)A.
- Intervention effect Feeding i(6)A to A. kolomikta callus increased io(6)Ade to 6 nmol/g in 8 hr.
- Intervention effect Feeding i(6)A to A. kolomikta callus did not increase Ribosylzeatin (io(6)A).
- Intervention effect Under N2, fed i(6)A accumulated in Actinidia kolomikta rather than being metabolized to i(6)Ade.
- Observation Among 30 woody plant species, the ability to accumulate io(6)Ade at 10 nmol/g or more in 24-hr feeds was observed in some groups.
- Observation The ability to accumulate io(6)Ade is restricted to certain systematic groups of woody plants, including order Ericales and families Oleaceae and Rubiaceae.