Intervention effectmodel-written summary

The reported culture condition produced a maximum callus induction frequency of 96.40%.

A claim from Establishing and Optimizing Callus Induction and Regeneration System from Mature Embryo of Indica Rice Variety Zhenshan 97B · 2013

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The reported culture condition produced a maximum callus induction frequency of 96.40%.

Taxon
Indica Rice Variety Zhenshan 97BOryza sativa Indica Groupt0
Organ / stage
Mature Embryot1
Treatment
MB + 2,4-D 1 mg·L- 1+ KT 4 mg·L- 1+ NAA 3 mg·L- 1with 12h photoperiod per daye0
Outcome
callus induction frequencye0
Direction
maximallye0
Value
96.40%e0

Not reportedMaterialGeneCompoundInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa Indica Group
Oryza sativa Indica Group · TaxonNo machine-readable author provided. Ranveig assumed (based on copyright claims). · Public domain · Wikimedia Commons ↗

Verbatim

e0

“callus induction frequency was maximally up to 96.40% with the subsequent plant regeneration rate being maximally up to 92.07% when cultured in MB + 2,4-D 1 mg·L- 1+ KT 4 mg·L- 1+ NAA 3 mg·L- 1with 12h photoperiod per day”

✓ located verbatim in the source · abstract, characters 297–518
t0

“Indica Rice Variety Zhenshan 97B”

✓ located verbatim in the source · title, characters 91–123
t1

“Establishing and Optimizing Callus Induction and Regeneration System from Mature Embryo of Indica Rice Variety Zhenshan 97B”

✓ located verbatim in the source · title, characters 0–123

Title

Establishing and Optimizing Callus Induction and Regeneration System from Mature Embryo of Indica Rice Variety Zhenshan 97B

Luo Tian-kua Zajiao shuidao 2013

Other claims from this paper (1)

Record rev-d78aa5a705f0640a40b6c4def7ccbce25d88032c04506be0261a5c0cc22fda27 · schema claims-v0.9 · position 1 in the model’s answer

kinetin · growth · Oryza sativa (rice)Results differ

59 papers: 52 positive, 7 negative or no effect

51 papers in the same direction as this claim · 6 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
    A high concentration of KT (over 1.5 mg/L) negatively affected callus induction in japonica rice microspore culture.

    Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.) · 2024

    Opposite direction

  2. negative
    Kinetin had an unclear or repressive effect on rice seed callus induction.

    Effect of Auxin Concentration on the Callus Induction from Various Organs of Rice Seedlings · 1976

    Opposite direction

  3. no effect
    GA and kinetin did not promote growth of rice buds when the medium lacked both of the other two growth substances.

    Studies on Branching Habits in Crop Plants : 9. Growth of tiller buds and apical buds of rice plants on agar medium as affected by concentration of kinetin and gibberellin · 1976

    Opposite direction

  4. no effect
    Paddy growth rate was about the same as the control.

    Physiology and bio - chemistry of germination of different types of seeds - iv - effect of certain chemicals on growth anddevelopment of cucumber, mungo, paddy, raddish and tomato plants. · 1984 · compared with control plants

    Opposite direction

  5. negative
    Kinetin supplemented medium produced very low or failed shoot regeneration in both varieties.

    Development of an efficient in vitro plant regeneration protocol for indica rice varieties (Oryza sativa L.) in the Mekong Delta of Vietnam · 2017

    Opposite direction

  6. negative
    KT inhibited primary root elongation with concentration and time.

    Exogenous 6-Benzyladenine and Kinetin Restrict Rice Seedling Root Growth, and Ag+ Partially Alleviates the Inhibition in Association with Ethylene-Related Responses and Increased Lipid Peroxidation · 2026

    Opposite direction

  7. no effect
    Kinetin alone did not produce a GA3 response on Tan ginbozu callus growth in a chemically defined medium.

    Amino Acids as Complementary Factors for the Growth and Redifferentiation of Rice Callus Tissues · 1972 · compared with chemically defined medium

    No difference from an alternative

  8. positive
    Forty-eight-hour dehydration plus kinetin and NAA gave 92% shoot regeneration.

    High Efficiency In vitro Whole Plant Regeneration via Desiccated Callus in Oryza sativa cv. MTU1010 · 2024

    Same direction

50 papers more in this group

See all 59 papers →

1-naphthaleneacetic acid · growth · Oryza sativa (rice)Results differ

71 papers: 65 positive, 6 negative or no effect

64 papers in the same direction as this claim · 6 in the opposite direction

  1. no effect
    Alpha-NAA had no effect on the measured adventitious root traits.

    Polar Auxin Transport Determines Adventitious Root Emergence and Growth in Rice · 2019

    Opposite direction

  2. no effect
    Exogenous NAA had no effect on secondary LR formation in rice

    A Strigolactone Signal Inhibits Secondary Lateral Root Development in Rice · 2019

    Opposite direction

  3. no effect
    NAA did not significantly change shoot growth.

    Growth regulation of cow1 rice mutant seedlings by blue light · 2010

    Opposite direction

  4. negative
    NAA was unsuitable for rice callus induction.

    Potensi Padi Bukit sebagai Alternatif kepada Padi Sawah · 2014

    Opposite direction

  5. negative
    NAA significantly reduced germination percentage and potentiality in both aged rice seed groups.

    Effect of the Hormone on the Vitality Recovery in Aged Rice Seed · 2003

    Opposite direction

  6. negative
    N6 medium with NAA 1.5 ppm failed to induce callus.

    In vitro selection of callus from local Badui rice plant ( · 2025

    Opposite direction

  7. positive
    Forty-eight-hour dehydration plus kinetin and NAA gave 92% shoot regeneration.

    High Efficiency In vitro Whole Plant Regeneration via Desiccated Callus in Oryza sativa cv. MTU1010 · 2024

    Same direction

  8. positive
    The maximum regeneration frequency of 100% was achieved on MS medium with BAP, Kin, NAA and TDZ within four weeks.

    Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas) · 2015

    Same direction

62 papers more in this group

See all 71 papers →

2,4-D · growth · Oryza sativa (rice)Positive and negative results

107 papers: 102 positive, 5 negative or no effect

101 papers in the same direction as this claim · 5 in the opposite direction

  1. negative
    2,4-D reduced adventitious root elongation rate.

    Polar Auxin Transport Determines Adventitious Root Emergence and Growth in Rice · 2019

    Opposite direction

  2. negative
    Fresh weight, water content, cellular water, and osmotic potentials decreased significantly in highly regenerable callus.

    Carbohydrate metabolism in rice during callus induction and shoot regeneration induced by osmotic stress · 2002

    Opposite direction

  3. no effect
    Low doses of 2,4-D had no effect on height, number of stems or active tillering of upland rice.

    Can 2,4-D promote the hormesis effect in upland rice? · 2022

    Opposite direction

  4. negative
    R4 medium with 2 mg/L 2,4-D had the lowest plantlet regeneration, 0%.

    Optimization of Plant Growth Regulators in Indirect Plant Regeneration in Rice Variety Nerica 3 Using Mature Seeds as Explant · 2024

    Opposite direction

  5. negative
    Without 2,4-D, rice callus did not form.

    Analysis of callus induction and regeneration in rice genotypes under different concentrations of 2,4-D · 2026

    Opposite direction

  6. positive
    A medium with 2.5 mg/l 2,4-D and 0.15 mg/l BAP gave maximal callus induction.

    Development of Agrobacterium-mediated transformation technology for mature seed-derived callus tissues of indica rice cultivar IR64 · 2012

    Same direction

  7. positive
    The best medium for callus induction in the four rice varieties was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l.

    Induksi Kalus dan Optimasi Regenerasi Empat Varietas Padi melalui Kultur In Vitro · 2016

    Same direction

  8. positive
    Yeast extract and 2,4-D were indispensable for the best growth of rice root callus.

    GROWTH OF CALLUS TISSUE FROM RICE-ROOT IN VIRTO · 1967

    Same direction

98 papers more in this group

See all 107 papers →

3 of 4 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.