Claim · model-written summary
Supplemented B5 produced more bean callus than the specified half-strength MS medium.
- Taxon
- beant0
- Treatment
- B5 medium supplemented with 1mg/1 KIN and 2mg/1 2,4-De0
- Outcome
- calluse0
- Direction
- much moree0
- Comparator
- half strength MS medium supplemented with 0.5 or 0.75mg/1 BA and 0.1 mg/1 NAAe0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Verbatim
e0“The B5 medium supplemented with 1mg/1 KIN and 2mg/1 2,4-D induced much more callus than half strength MS medium supplemented with 0.5 or 0.75mg/1 BA and 0.1 mg/1 NAA.”
t0“bean”
Title
Production of transgenic bean callus via genetic transformation by DNA-coated tungsten particles
E. Eissa Ahmed International Journal of Horticultural Science 2003. doi:10.31421/ijhs/9/3-4/404
Other claims from this paper (6)
- Intervention effect B5 medium with KIN and 2,4-D was best for bean callus induction.
- Observation Bean callus developed and was maintained on supplemented B5 medium.
- Method / resource GeneboosterTM produced transient gene expression in bean callus.
- Method / resource GeneboosterTM bombardment yielded kanamycin-resistant and mannitol-tolerant bean calli.
- Observation PCR verified integration of the mannitol-dehydrogenase gene in bean callus.
- Observation PCR confirmed insertion of mtl in mannitol-tolerant callus lines.