By organism › Bacteria › Phyllobacteriaceae › Mesorhizobium loti

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

1533 M. loti proteins were identified under free-living conditions.

Taxon: M. lotiMesorhizobium lotie0
Outcome: proteinse0
Value: 1,533e0
Qualifier: free-livinge0

Verbatim

e0
“We identified 1,533 and 847 proteins for M. loti under free-living and symbiotic conditions, respectively.”
✓ located verbatim in the source · abstract, characters 1001–1107

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

847 M. loti proteins were identified under symbiotic conditions.

Taxon: M. lotiMesorhizobium lotie0
Outcome: proteinse0
Value: 847e0
Qualifier: symbiotic conditionse0

Verbatim

e0
“We identified 1,533 and 847 proteins for M. loti under free-living and symbiotic conditions, respectively.”
✓ located verbatim in the source · abstract, characters 1001–1107

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Many central-carbon metabolic enzymes were detected in both conditions.

Taxon: Mesorhizobium lotiMesorhizobium lotit0
Outcome: enzymes involved in the central carbon metabolic pathwaye0
Qualifier: commonly detected under both conditionse0

Verbatim

e0
“Pathway analysis by Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that many of the enzymes involved in the central carbon metabolic pathway were commonly detected under both conditions.”
✓ located verbatim in the source · abstract, characters 1108–1303

t0
“Mesorhizobium loti”
✓ located verbatim in the source · title, characters 33–51

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Symbiosis-island proteins were uniquely detected under symbiotic conditions.
Method / resource Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.
Observation Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Method / resource The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.
Observation M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Symbiosis-island proteins were uniquely detected under symbiotic conditions.

Taxon: Mesorhizobium lotiMesorhizobium lotit0
Outcome: proteins encoded in the symbiosis islande0
Qualifier: uniquely detected under the symbiotic conditione0

Verbatim

e0
“The proteins encoded in the symbiosis island, the transmissible chromosomal region that includes the genes that are highly upregulated under the symbiotic condition, were uniquely detected under the symbiotic condition.”
✓ located verbatim in the source · abstract, characters 1304–1523

t0
“Mesorhizobium loti”
✓ located verbatim in the source · title, characters 33–51

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Many central-carbon metabolic enzymes were detected in both conditions.
Method / resource Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.
Observation Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Method / resource The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.
Observation M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.

Taxon: Mesorhizobium lotiMesorhizobium lotit0
Outcome: features of the symbiotic conditione0
Comparator: transcriptome analysise0

Verbatim

e0
“The features of the symbiotic condition that have been reported by transcriptome analysis were confirmed at the protein level by proteome analysis.”
✓ located verbatim in the source · abstract, characters 1524–1671

t0
“Mesorhizobium loti”
✓ located verbatim in the source · title, characters 33–51

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Many central-carbon metabolic enzymes were detected in both conditions.
Observation Symbiosis-island proteins were uniquely detected under symbiotic conditions.
Observation Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Method / resource The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.
Observation M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.

Taxon: Mesorhizobium lotiMesorhizobium lotit0
Outcome: genes of the proteins involved in cell surface structuree0
Direction: repressede0
Qualifier: symbiotic nitrogen-fixing conditione0

Verbatim

e0
“In addition, the genes of the proteins involved in cell surface structure were repressed under the symbiotic nitrogen-fixing condition.”
✓ located verbatim in the source · abstract, characters 1672–1807

t0
“Mesorhizobium loti”
✓ located verbatim in the source · title, characters 33–51

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Many central-carbon metabolic enzymes were detected in both conditions.
Observation Symbiosis-island proteins were uniquely detected under symbiotic conditions.
Method / resource Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Method / resource The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.
Observation M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.

Taxon: Mesorhizobium lotiMesorhizobium lotit0
Outcome: difference in phenotypese0
Qualifier: appearede0
Comparator: free-livinge0

Verbatim

e0
“The obtained protein profile appeared to reflect the difference in phenotypes under the free-living and symbiotic conditions.”
✓ located verbatim in the source · abstract, characters 1941–2066

t0
“Mesorhizobium loti”
✓ located verbatim in the source · title, characters 33–51

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Many central-carbon metabolic enzymes were detected in both conditions.
Observation Symbiosis-island proteins were uniquely detected under symbiotic conditions.
Method / resource Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.
Observation Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.
Observation M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

M. loti changed metabolism and cell-surface structure according to surrounding conditions.

Taxon: M. lotiMesorhizobium lotie0
Outcome: metabolism and cell surface structuree0
Qualifier: in accordance with the surrounding conditionse0

Verbatim

e0
“M. loti changed its metabolism and cell surface structure in accordance with the surrounding conditions.”
✓ located verbatim in the source · abstract, characters 2294–2398

Title

Disclosure of the differences of Mesorhizobium loti under the free-living and symbiotic conditions by comparative proteome analysis without bacteroid isolation

Yohei Tatsukami; Mami Nambu; Hironobu Morisaka; et al. BMC Microbiology 2013. doi:10.1186/1471-2180-13-180

Other claims from this paper (10)

Method / resource 1533 M. loti proteins were identified under free-living conditions.
Method / resource 847 M. loti proteins were identified under symbiotic conditions.
Observation Many central-carbon metabolic enzymes were detected in both conditions.
Observation Symbiosis-island proteins were uniquely detected under symbiotic conditions.
Method / resource Proteomics confirmed previously transcriptome-reported features of the symbiotic condition at protein level.
Observation Genes encoding cell-surface-structure proteins were repressed under symbiotic nitrogen fixation.
Observation FPP biosynthesis was found only in symbiotic rhizobia.
Method / resource The protein profile appeared to reflect phenotypic differences between free-living and symbiotic conditions.
Observation KEGG analysis indicated rhizobial cell-surface structure differed substantially between conditions.
Mechanism Rhizobia might provide FPP to the host as a secondary-metabolism source.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Wild-type M. loti R7A produced high and low molecular weight polysaccharide fractions, while most mutants produced only low molecular weight fractions.

Taxon: M. loti R7AMesorhizobium lotie0
Outcome: high molecular weight EPS and lower molecular weight (LMW) polysaccharide fractionse0
Comparator: most mutant strainse0

Verbatim

e0
“The wild-type M. loti R7A produced both high molecular weight EPS and lower molecular weight (LMW) polysaccharide fractions whereas most mutant strains produced only LMW fractions”
✓ located verbatim in the source · abstract, characters 429–608

Title

Conditional Requirement for Exopolysaccharide in the Mesorhizobium–Lotus Symbiosis

Simon J. Kelly; Artur Muszyński; Yasuyuki Kawaharada; et al. Molecular Plant-Microbe Interactions 2012. doi:10.1094/mpmi-09-12-0227-r

Other claims from this paper (8)

Observationobservedoriginal resultmeasured

Claim · model-written summary

M. loti cells respond to environmental and symbiotic cues with transient elevations in intracellular free Ca2+ concentration.

Taxon: M. lotiMesorhizobium lotie0
Outcome: transient elevations in intracellular free Ca(2+) concentratione0

Verbatim

e0
“M. loti cells respond to environmental and symbiotic cues through transient elevations in intracellular free Ca(2+) concentration”
✓ located verbatim in the source · abstract, characters 663–792

Title

Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti

Roberto Moscatiello; Sara Alberghini; Andrea Squartini; et al. BMC Microbiology 2009. doi:10.1186/1471-2180-9-206

Other claims from this paper (3)

Mesorhizobium loti 13 claims

13 claims