Abstract
Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.
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Claims 11
Claim · model-written summary
OsALS1 is regulated by ART1.
- Taxon
- rice
Oryza sativat0 - Gene
- Os03g0755100 (OsALS1)e0
- Outcome
- regulated by ART1e0
- Qualifier
- ART1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1”
t0“rice”
Claim · model-written summary
OsALS1 encodes a half-size ABC transporter in the TAP subgroup.
- Taxon
- riceOryza sativat0
- Gene
- OsALS1e0
- Outcome
- half-size ABC transportere0
- Qualifier
- TAP (transporter associated with antigen processing) sub-groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group.”
t0“rice”
Claim · model-written summary
Al rapidly and specifically induced OsALS1 expression in roots.
- Taxon
- riceOryza sativat0
- Organ / stage
- rootse0
- Treatment
- Ale0
- Gene
- OsALS1e0
- Outcome
- Expression of OsALS1e0
- Direction
- inducede0
- Comparator
- other metals or low pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH.”
t0“rice”
Claim · model-written summary
OsALS1 localized in all root cells and to the tonoplast.
- Taxon
- riceOryza sativat0
- Organ / stage
- all cells of the rootse0
- Gene
- OsALS1e0
- Outcome
- localizede0
- Qualifier
- tonoplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast.”
t0“rice”
Claim · model-written summary
OsALS1 expression and cellular localization differed from Arabidopsis AtALS1.
- Taxon
- riceOryza sativat0
- Gene
- AtALS1e0
- Outcome
- expression patterns and cell specificity of localizatione0
- Direction
- differente0
- Comparator
- Arabidopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis.”
t0“rice”
Claim · model-written summary
OsALS1 knockout increased Al sensitivity significantly.
- Taxon
- riceOryza sativat0
- Material
- three independent linese0
- Treatment
- Knockout of OsALS1e0
- Gene
- OsALS1e0
- Outcome
- sensitivity to Ale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al”
t0“rice”
Claim · model-written summary
OsALS1 knockout did not affect sensitivity to other metals or low pH.
- Taxon
- riceOryza sativat0
- Treatment
- Knockout of OsALS1e0
- Outcome
- sensitivity to other metals and low pHe0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH.”
t0“rice”
Claim · model-written summary
Root-tip cell-sap Al levels did not differ between wild type and mutants.
- Taxon
- riceOryza sativat0
- Outcome
- Al levels in the cell sap of root tipse0
- Direction
- no differencee0
- Comparator
- wild-type and the mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants”
t0“rice”
Claim · model-written summary
OsALS1 mutants accumulated more cytosolic and nuclear Al than wild type.
- Taxon
- riceOryza sativat0
- Material
- mutantse0
- Outcome
- Al in the cytosol and nucleuse0
- Direction
- moree0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutants accumulated more Al in the cytosol and nucleus than the wild-type.”
t0“rice”
Claim · model-written summary
Yeast expressing OsALS1 showed increased Al sensitivity attributed to mislocalization.
- Taxon
- riceOryza sativat0
- Gene
- OsALS1e0
- Outcome
- increased Al sensitivitye0
- Qualifier
- mis-localizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization.”
t0“rice”
Claim · model-written summary
The authors infer that tonoplast OsALS1 sequesters Al into vacuoles for internal detoxification.
- Taxon
- riceOryza sativat0
- Gene
- OsALS1e0
- Outcome
- internal detoxification of Ale0
- Qualifier
- sequestration of Al into the vacuolese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.”
t0“rice”
The same questions in other papers
aluminium atom · molecular: expression · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.