Abstract
-transcription factor OsART1 plays a crucial role in regulating Al tolerance in rice by modulating the expression of specific genes. However, the posttranscriptional regulation of OsART1 remains poorly understood. In this study, we identified and characterised OsWRKY64, which interacts with OsART1. OsWRKY64 was localised in the nucleus. Tissue expression analysis indicated that OsWRKY64 was primarily expressed in the roots, and its transcription and protein accumulation were significantly induced by Al. The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1, which consequently led to phenotypes sensitive or resistant to Al, respectively. Further analysis indicated that OsWRKY64 interacted with OsART1 to promote its stability, thereby accelerating the transcriptional activity of OsART1 on its downstream genes. Additionally, OsART1 upregulated OsWRKY64 expression by directly binding to the OsWRKY64 promoter, thus forming a negative feedback loop between OsART1 and OsWRKY64. Overall, our results demonstrated that OsWRKY64 influences Al resistance by interacting with and stabilising OsART1 to accelerate its transcriptional activity.
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Claims 13
Claim · model-written summary
OsWRKY64 interacted with OsART1.
- Taxon
- Rice
Oryza sativat0 - Gene
- OsWRKY64e0
- Outcome
- interacts with OsART1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified and characterised OsWRKY64, which interacts with OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 localized to the nucleus.
- Taxon
- RiceOryza sativat0
- Gene
- OsWRKY64e0
- Outcome
- localised in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY64 was localised in the nucleus”
t0“Rice”
Claim · model-written summary
OsWRKY64 was primarily expressed in roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse0
- Gene
- OsWRKY64e0
- Outcome
- primarily expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY64 was primarily expressed in the roots”
t0“Rice”
Claim · model-written summary
Al significantly induced OsWRKY64 transcription.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its transcription and protein accumulation were significantly induced by Al”
e1“we identified and characterised OsWRKY64, which interacts with OsART1”
t0“Rice”
Claim · model-written summary
Al significantly induced OsWRKY64 protein accumulation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its transcription and protein accumulation were significantly induced by Al”
e1“we identified and characterised OsWRKY64, which interacts with OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 mutants had decreased OsART1 regulated gene expression.
- Taxon
- RiceOryza sativat0
- Material
- oswrky64 mutantse0
- Treatment
- oswrky64 mutantse0
- Gene
- OsART1e0
- Outcome
- expression of genes regulated by OsART1e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 overexpression lines had increased OsART1 regulated gene expression.
- Taxon
- RiceOryza sativat0
- Material
- OsWRKY64 overexpression linese0
- Treatment
- OsWRKY64 overexpression linese0
- Gene
- OsART1e0
- Outcome
- expression of genes regulated by OsART1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 mutants were sensitive to Al.
- Taxon
- RiceOryza sativat0
- Material
- oswrky64 mutantse1
- Outcome
- sensitive or resistant to Ale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenotypes sensitive or resistant to Al, respectively”
e1“The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 overexpression lines were resistant to Al.
- Taxon
- RiceOryza sativat0
- Material
- OsWRKY64 overexpression linese1
- Outcome
- sensitive or resistant to Ale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenotypes sensitive or resistant to Al, respectively”
e1“The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1”
t0“Rice”
Claim · model-written summary
OsWRKY64 interaction promoted OsART1 stability.
- Taxon
- RiceOryza sativat0
- Gene
- OsWRKY64e0
- Outcome
- stabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY64 interacted with OsART1 to promote its stability”
t0“Rice”
Claim · model-written summary
OsWRKY64 accelerated OsART1 transcriptional activity on downstream genes.
- Taxon
- RiceOryza sativat0
- Gene
- OsART1e0
- Outcome
- transcriptional activitye0
- Direction
- acceleratinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thereby accelerating the transcriptional activity of OsART1 on its downstream genes”
t0“Rice”
Claim · model-written summary
OsART1 directly bound the OsWRKY64 promoter.
- Taxon
- RiceOryza sativat0
- Gene
- OsART1e0
- Outcome
- directly binding to the OsWRKY64 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsART1 upregulated OsWRKY64 expression by directly binding to the OsWRKY64 promoter”
t0“Rice”
Claim · model-written summary
OsART1 upregulated OsWRKY64 expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsART1e0
- Outcome
- OsWRKY64 expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsART1 upregulated OsWRKY64 expression by directly binding to the OsWRKY64 promoter”
t0“Rice”
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