Abstract
The negative charges of cell wall pectin molecules attributed by pectin methylesterase (PME, EC 3.1.1.11) contribute to Al binding capacity. We examined the expression profiles of 35 members of the PME gene family in the root apex of an Al-sensitive rice 'Zhefu802' under Al stress. While root elongation was inhibited by 40% after 3-h exposure to 25 µM Al, cell wall PME activity and the abundance of eight PME genes transcripts were increased. The same Al treatment which had almost no effect on root elongation of an Al-resistant rice ssp. japonica 'Nipponbare' did not change the expression patterns of these eight PME genes. However, when Al concentration was increased to 50 µM, by which the root elongation of 'Nipponbare' was inhibited by 40% too, the expression of these PME genes were also upregulated except two genes with no signal. These suggest a possible correlation between the upregulated genes and Al-induced inhibition of root elongation in rice. Furthermore, these eight PME genes behaved differently when subjected to CdCl2 and LaCl3 treatments, implying the specificity of different PME genes in response to different metal toxicities. The transgenic rice overexpressing one of these eight PME genes OsPME14 showed higher PME activity and Al content in root tip cell wall, and became more sensitive to Al stress, verifying the involvement of the specific PME gene in Al toxicity. Therefore, our results provided the molecular evidence to connect the expression of specific PME genes with the Al-induced inhibition of root elongation in rice.
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Claims 11
Claim · model-written summary
25 µM Al for three hours inhibited Zhefu802 root elongation by 40%.
- Taxon
- rice
Oryza sativat0 - Material
- Zhefu802e1
- Treatment
- 25 µM Ale0
- Outcome
- root elongatione0
- Direction
- inhibitede0
- Value
- 40%e0
- Qualifier
- 3-h exposuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While root elongation was inhibited by 40% after 3-h exposure to 25 µM Al”
t0“rice”
e1“an Al-sensitive rice 'Zhefu802' under Al stress”
Claim · model-written summary
25 µM Al increased root cell wall PME activity in Zhefu802.
- Taxon
- riceOryza sativat0
- Material
- Zhefu802e1
- Treatment
- 25 µM Ale1
- Gene
- PMEe0
- Outcome
- cell wall PME activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell wall PME activity and the abundance of eight PME genes transcripts were increased.”
t0“rice”
e1“an Al-sensitive rice 'Zhefu802' under Al stress. While root elongation was inhibited by 40% after 3-h exposure to 25 µM Al”
Claim · model-written summary
25 µM Al increased transcript abundance of eight PME genes in Zhefu802.
- Taxon
- riceOryza sativat0
- Material
- Zhefu802e1
- Treatment
- 25 µM Ale1
- Gene
- PMEe0
- Outcome
- abundance of eight PME genes transcriptse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell wall PME activity and the abundance of eight PME genes transcripts were increased.”
t0“rice”
e1“an Al-sensitive rice 'Zhefu802' under Al stress. While root elongation was inhibited by 40% after 3-h exposure to 25 µM Al”
Claim · model-written summary
25 µM Al had almost no effect on Nipponbare root elongation.
- Taxon
- riceOryza sativae0
- Material
- Nipponbaree0
- Treatment
- Al treatmente0
- Outcome
- root elongatione0
- Direction
- almost no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The same Al treatment which had almost no effect on root elongation of an Al-resistant rice ssp. japonica 'Nipponbare'”
Claim · model-written summary
25 µM Al did not alter expression patterns of the eight PME genes in Nipponbare.
- Taxon
- riceOryza sativat0
- Material
- Nipponbaree1
- Treatment
- Al treatmente1
- Gene
- PMEe0
- Outcome
- expression patternse0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not change the expression patterns of these eight PME genes.”
t0“rice”
e1“The same Al treatment which had almost no effect on root elongation of an Al-resistant rice ssp. japonica 'Nipponbare'”
Claim · model-written summary
50 µM Al inhibited Nipponbare root elongation by 40%.
- Taxon
- riceOryza sativat0
- Material
- Nipponbaree0
- Treatment
- 50 µMe0
- Outcome
- root elongatione0
- Direction
- inhibitede0
- Value
- 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when Al concentration was increased to 50 µM, by which the root elongation of 'Nipponbare' was inhibited by 40% too”
t0“rice”
Claim · model-written summary
At 50 µM Al, the PME genes in Nipponbare were upregulated except for two genes without signal.
- Taxon
- riceOryza sativat0
- Material
- Nipponbaree1
- Treatment
- 50 µMe1
- Gene
- PMEe0
- Outcome
- expression of these PME genese0
- Direction
- upregulatede0
- Qualifier
- except two genes with no signale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of these PME genes were also upregulated except two genes with no signal.”
t0“rice”
e1“when Al concentration was increased to 50 µM, by which the root elongation of 'Nipponbare' was inhibited by 40% too”
Claim · model-written summary
Eight PME genes responded differently to CdCl2 and LaCl3 treatments.
- Taxon
- riceOryza sativat0
- Treatment
- CdCl2 and LaCl3 treatmentse0
- Gene
- PMEe0
- Outcome
- eight PME genese0
- Direction
- differentlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these eight PME genes behaved differently when subjected to CdCl2 and LaCl3 treatments”
t0“rice”
Claim · model-written summary
OsPME14 overexpression increased rice root tip cell wall PME activity.
- Taxon
- riceOryza sativae0
- Organ / stage
- root tip cell walle0
- Treatment
- overexpressinge0
- Gene
- OsPME14e0
- Outcome
- PME activitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic rice overexpressing one of these eight PME genes OsPME14 showed higher PME activity and Al content in root tip cell wall, and became more sensitive to Al stress”
Claim · model-written summary
OsPME14 overexpression increased Al content in rice root tip cell wall.
- Taxon
- riceOryza sativae0
- Organ / stage
- root tip cell walle0
- Treatment
- overexpressinge0
- Gene
- OsPME14e0
- Outcome
- Al contente0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic rice overexpressing one of these eight PME genes OsPME14 showed higher PME activity and Al content in root tip cell wall, and became more sensitive to Al stress”
Claim · model-written summary
OsPME14 overexpression made rice more sensitive to Al stress.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressinge0
- Gene
- OsPME14e0
- Outcome
- sensitive to Al stresse0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic rice overexpressing one of these eight PME genes OsPME14 showed higher PME activity and Al content in root tip cell wall, and became more sensitive to Al stress”
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