Abstract
The basis for the salt tolerant phenotype of a line of Medicago sativa (alfalfa) cells (HG2-N1) derived by selection from a salt sensitive line (HG2) was studied. The salt tolerant HG2-N1 cell line shows eleven fold elevated chlorophyll content over that of the parent salt sensitive HG2 cell line, with an additional two fold increase in chlorophyll levels when the cells are grown in 1% NaCl. In this study, we demonstrate that the chlorophyll accumulation and response to salt was associated with large increases in the two photosynthesis related mRNAs, rbcL (ribulose-l,5-bis-phosphate carboxylase [Rubisco] large subunit) and rbcS (Rubisco small subunit) and a substantial increase in the activity of the holoenzyme. The salinity-induced increase in catalytically competent Rubisco protein in the salt tolerant cell line was highly responsive to light and correlated with the salt tolerant phenotype. In addition, NaCl stimulated rbcL and rbcS mRNA and Rubisco accumulation in dark grown salt tolerant cells, indicating that salt could substitute to some degree for light in stimulating increases in specific mRNA and protein concentrations. Increased photosynthetic competence associated with these increased protein levels was apparently important in contributing to the salt tolerant phenotype of HG2-N1, since PS II electron transport inhibitors (DCMU, cyanazine) were found to significantly reduce the growth of this cell line in the presence of salt, but not in the absence of salt. These results suggest that the salt-induced increase in mRNA and protein accumulation involved in photosynthesis may play a significant role in the salt tolerant capability of HG2-N1 alfalfa cells.
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Claims 9
Claim · model-written summary
HG2-N1 cells have elevenfold higher chlorophyll content than the salt-sensitive HG2 line.
- Taxon
- Medicago sativa
Medicago sativae1 - Material
- HG2-N1e0
- Outcome
- chlorophyll contente0
- Direction
- elevatede0
- Value
- eleven folde0
- Comparator
- parent salt sensitive HG2 cell linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salt tolerant HG2-N1 cell line shows eleven fold elevated chlorophyll content over that of the parent salt sensitive HG2 cell line”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Growth in 1% NaCl gave a further twofold increase in chlorophyll levels.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- 1% NaCle0
- Outcome
- chlorophyll levelse0
- Direction
- increasee0
- Value
- two folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with an additional two fold increase in chlorophyll levels when the cells are grown in 1% NaCl”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Salt response was associated with large increases in rbcL mRNA.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- salte0
- Gene
- rbcLe0
- Outcome
- photosynthesis related mRNAse0
- Direction
- large increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorophyll accumulation and response to salt was associated with large increases in the two photosynthesis related mRNAs, rbcL”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Salt response was associated with large increases in rbcS mRNA.
- Taxon
- Medicago sativaMedicago sativae2
- Treatment
- salte0
- Gene
- rbcSe1
- Outcome
- photosynthesis related mRNAse0
- Direction
- large increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorophyll accumulation and response to salt was associated with large increases in the two photosynthesis related mRNAs”
e1“and rbcS (Rubisco small subunit)”
e2“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Rubisco holoenzyme activity increased substantially.
- Taxon
- Medicago sativaMedicago sativae1
- Outcome
- activity of the holoenzymee0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a substantial increase in the activity of the holoenzyme”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Salinity-induced Rubisco protein increase is highly responsive to light and correlated with salt tolerance.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- salinitye0
- Outcome
- catalytically competent Rubisco proteine0
- Direction
- increasee0
- Qualifier
- highly responsive to light and correlated with the salt tolerant phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The salinity-induced increase in catalytically competent Rubisco protein in the salt tolerant cell line was highly responsive to light and correlated with the salt tolerant phenotype”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
NaCl stimulated rbcL and rbcS mRNA and Rubisco accumulation in dark-grown salt-tolerant cells.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- NaCle0
- Gene
- rbcL and rbcSe0
- Outcome
- rbcL and rbcS mRNA and Rubisco accumulatione0
- Direction
- stimulatede0
- Qualifier
- dark grown salt tolerant cellse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NaCl stimulated rbcL and rbcS mRNA and Rubisco accumulation in dark grown salt tolerant cells”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
PS II electron transport inhibitors significantly reduced growth of the cell line in salt but not without salt.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- PS II electron transport inhibitors (DCMU, cyanazine)e0
- Outcome
- growth of this cell linee0
- Direction
- reducee0
- Qualifier
- in the presence of salt, but not in the absence of salte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PS II electron transport inhibitors (DCMU, cyanazine) were found to significantly reduce the growth of this cell line in the presence of salt, but not in the absence of salt”
e1“Alfalfa (Medicago sativa) Cells”
Claim · model-written summary
Salt-induced accumulation of photosynthesis mRNA and protein may contribute to the salt tolerance of HG2-N1.
- Taxon
- Medicago sativaMedicago sativae1
- Outcome
- salt tolerant capabilitye0
- Qualifier
- salt-induced increase in mRNA and protein accumulation involved in photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the salt-induced increase in mRNA and protein accumulation involved in photosynthesis may play a significant role in the salt tolerant capability of HG2-N1 alfalfa cells”
e1“Alfalfa (Medicago sativa) Cells”
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