Abstract
Multivesicular bodies (MVBs) are unique endosomes containing vesicles in the lumen and play critical roles in many cellular processes. We have recently shown that Arabidopsis (Arabidopsis thaliana) Lyst-Interacting Protein5 (LIP5), a positive regulator of the Suppressor of K(+) Transport Growth Defect1 (SKD1) AAA ATPase in MVB biogenesis, is a critical target of the mitogen-activated protein kinases MPK3 and MPK6 and plays an important role in the plant immune system. In this study, we report that the LIP5-regulated MVB pathway also plays a critical role in plant responses to abiotic stresses. Disruption of LIP5 causes compromised tolerance to both heat and salt stresses. The critical role of LIP5 in plant tolerance to abiotic stresses is dependent on its ability to interact with Suppressor of K(+) Transport Growth Defect1. When compared with wild-type plants, lip5 mutants accumulate increased levels of ubiquitinated protein aggregates and NaCl under heat and salt stresses, respectively. Further analysis using fluorescent dye and MVB markers reveals that abiotic stress increases the formation of endocytic vesicles and MVBs in a largely LIP5-dependent manner. LIP5 is also required for the salt-induced increase of intracellular reactive oxygen species, which have been implicated in signaling of salt stress responses. Basal levels of LIP5 phosphorylation by MPKs and the stability of LIP5 are elevated by salt stress, and mutation of MPK phosphorylation sites in LIP5 reduces the stability and compromises the ability to complement the lip5 salt-sensitive mutant phenotype. These results collectively indicate that the MVB pathway is positively regulated by pathogen/stress-responsive MPK3/6 through LIP5 phosphorylation and plays a critical role in broad plant responses to biotic and abiotic stresses.
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Claims 9
Claim · model-written summary
Disrupting LIP5 compromises heat tolerance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae1 - Treatment
- Disruption of LIP5e0
- Gene
- LIP5e0
- Outcome
- tolerance to both heat and salt stressese0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of LIP5 causes compromised tolerance to both heat and salt stresses.”
e1“Arabidopsis (Arabidopsis thaliana) Lyst-Interacting Protein5 (LIP5)”
Claim · model-written summary
LIP5 interaction with SKD1 is required for abiotic stress tolerance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Gene
- LIP5e0
- Outcome
- plant tolerance to abiotic stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The critical role of LIP5 in plant tolerance to abiotic stresses is dependent on its ability to interact with Suppressor of K(+) Transport Growth Defect1.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
lip5 mutants accumulate more ubiquitinated protein aggregates under heat stress.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Material
- lip5 mutantse0
- Treatment
- heate0
- Outcome
- ubiquitinated protein aggregatese0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When compared with wild-type plants, lip5 mutants accumulate increased levels of ubiquitinated protein aggregates and NaCl under heat and salt stresses, respectively.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
lip5 mutants accumulate more NaCl under salt stress.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Material
- lip5 mutantse0
- Treatment
- salte0
- Outcome
- NaCle0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When compared with wild-type plants, lip5 mutants accumulate increased levels of ubiquitinated protein aggregates and NaCl under heat and salt stresses, respectively.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Abiotic stress increases endocytic vesicles and MVB formation largely through LIP5.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- abiotic stresse0
- Gene
- LIP5e0
- Outcome
- formation of endocytic vesicles and MVBse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“abiotic stress increases the formation of endocytic vesicles and MVBs in a largely LIP5-dependent manner.”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
LIP5 is required for salt-induced intracellular reactive oxygen species accumulation.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- salte0
- Gene
- LIP5e0
- Outcome
- intracellular reactive oxygen speciese0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LIP5 is also required for the salt-induced increase of intracellular reactive oxygen species”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Salt stress elevates basal LIP5 phosphorylation by MPKs.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- salt stresse0
- Gene
- LIP5e0
- Outcome
- LIP5 phosphorylation by MPKse0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basal levels of LIP5 phosphorylation by MPKs and the stability of LIP5 are elevated by salt stress”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Salt stress elevates LIP5 stability.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- salt stresse0
- Gene
- LIP5e0
- Outcome
- stability of LIP5e0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basal levels of LIP5 phosphorylation by MPKs and the stability of LIP5 are elevated by salt stress”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Mutation of MPK phosphorylation sites reduces LIP5 stability and complementation of salt sensitivity.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae1
- Treatment
- mutation of MPK phosphorylation sites in LIP5e0
- Gene
- LIP5e0
- Outcome
- stabilitye0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of MPK phosphorylation sites in LIP5 reduces the stability and compromises the ability to complement the lip5 salt-sensitive mutant phenotype”
e1“Arabidopsis (Arabidopsis thaliana)”
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