Abstract
Reduced glutathione (GSH) is required for root development, but its functions are not characterized. The effects of GSH depletion on root development were therefore studied in relation to auxin and strigolactone (SL) signalling using a combination of molecular genetic approaches and pharmacological techniques. Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-), but not by the GSH synthesis inhibitor, buthionine sulfoximine (BSO). BSO-induced GSH depletion therefore did not influence root architecture in the same way as genetic impairment. Root glutathione contents were similar in the wild-type seedlings and max3-9 and max4-1 mutants that are deficient in SL synthesis and in the SL-signalling mutant, max2-1. BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool to a similar extent in the wild-type and SL synthesis mutants, with no effect on LR density. The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings, but this increase was absent from max2-1. Taken together, these data establish a link between SLs and the GSH pool that occurs in a MAX2-dependent manner.
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Claims 9
Claim · model-written summary
Lateral root density was significantly decreased in GSH synthesis mutants.
- Material
- GSH synthesis mutants (cad2-1, pad2-, rax1-)e0
- Outcome
- Lateral root (LR) densitye0
- Direction
- decreasede0
Not reportedTaxon
Verbatim
e0“Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-)”
Claim · model-written summary
BSO did not decrease lateral root density.
Not reportedTaxonComparatorDirection
Verbatim
e0“but not by the GSH synthesis inhibitor, buthionine sulfoximine (BSO)”
t0“root architecture”
e1“Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-)”
Claim · model-written summary
Root glutathione content was similar in wild-type and strigolactone synthesis or signaling mutants.
- Material
- max3-9 and max4-1 mutantse0
- Outcome
- Root glutathione contentse0
- Direction
- similare0
- Comparator
- wild-type seedlingse0
Not reportedTaxon
Verbatim
e0“Root glutathione contents were similar in the wild-type seedlings and max3-9 and max4-1 mutants that are deficient in SL synthesis and in the SL-signalling mutant, max2-1.”
Claim · model-written summary
BSO depleted tissue GSH similarly in wild type and SL synthesis mutants.
- Material
- SL synthesis mutantse0
- Treatment
- BSO-dependent inhibition of GSH synthesise0
- Outcome
- tissue GSH poole0
- Direction
- depletede0
- Comparator
- wild-typee0
Not reportedTaxon
Verbatim
e0“BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool to a similar extent in the wild-type and SL synthesis mutants”
Claim · model-written summary
BSO-induced GSH depletion had no effect on lateral root density.
Not reportedTaxonComparator
Verbatim
e0“with no effect on LR density”
t0“root architecture”
e1“BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool”
Claim · model-written summary
GR24 increased root glutathione in wild-type seedlings.
Not reportedTaxonComparator
Verbatim
e0“The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings”
Claim · model-written summary
GR24 increased root glutathione in max3-9 and max4-1 seedlings.
- Material
- max3-9 and max4-1 seedlingse0
- Treatment
- SL analogue GR24e0
- Outcome
- root glutathionee0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings”
Claim · model-written summary
The GR24-associated increase in root glutathione was absent in max2-1.
Not reportedTaxonComparator
Verbatim
e0“but this increase was absent from max2-1”
t0“root architecture”
e1“The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings”
Claim · model-written summary
The data indicate a MAX2-dependent link between strigolactones and the GSH pool.
Not reportedTaxon
Verbatim
e0“these data establish a link between SLs and the GSH pool that occurs in a MAX2-dependent manner”