Abstract
BACKGROUND: Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture. Here, the mutations in the glutamine synthetase (GS) gene are described to understand the evidence pointing to the functional role of the GS gene in the herbicide sensitivity of the mutant cells. RESULTS: Based on the I(50) values, dose-response experiments at the cell level showed that the resistance ratio of the resistant cell was 50-fold, whereas the in vitro inhibition of GS activity required a 4.56-fold greater concentration of glufosinate in the resistant cell than in the untreated control. Comparison of the nucleotide sequences identified nine point differences in the GS gene between the resistant and untreated cells, leading to eight amino acid substitutions in the deduced polypeptide sequence. Northern hybridization of the GS mRNA showed that the accumulation of GS gene mRNA transcript in resistant cells was higher than that in the untreated cells. CONCLUSION: Changes in sensitivity to glufosinate have been related to mutations at the binding site of the herbicide on the glutamine synthetase. His(249) is one of the residues implicated in the binding domain for the substrate and inhibitor, and hence the exchange of this residue with tyrosine plays a role in lowering the sensitivity of the mutated enzyme.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
The resistant soybean cell line had a 50-fold resistance ratio to glufosinate.
- Taxon
- soybean
Glycine maxe0 - Material
- resistant celle1
- Outcome
- resistance ratioe1
- Value
- 50-folde1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e1“dose-response experiments at the cell level showed that the resistance ratio of the resistant cell was 50-fold”
Claim · model-written summary
In vitro GS inhibition by glufosinate required a 4.56-fold greater concentration in the resistant cell than in the untreated control.
- Taxon
- soybeanGlycine maxe0
- Material
- resistant celle2
- Treatment
- glufosinatee2
- Gene
- GSe2
- Outcome
- in vitro inhibition of GS activitye2
- Direction
- greatere2
- Value
- 4.56-folde2
- Comparator
- untreated controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e2“the in vitro inhibition of GS activity required a 4.56-fold greater concentration of glufosinate in the resistant cell than in the untreated control”
Claim · model-written summary
Nine point differences in the GS gene were identified between resistant and untreated cells.
- Taxon
- soybeanGlycine maxe0
- Gene
- GS genee3
- Outcome
- point differencese3
- Value
- ninee3
- Comparator
- untreated cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e3“Comparison of the nucleotide sequences identified nine point differences in the GS gene between the resistant and untreated cells, leading to eight amino acid substitutions in the deduced polypeptide sequence”
Claim · model-written summary
Eight amino acid substitutions in the deduced GS polypeptide were found between resistant and untreated cells.
- Taxon
- soybeanGlycine maxe0
- Gene
- GS genee3
- Outcome
- amino acid substitutionse3
- Value
- eighte3
- Qualifier
- in the deduced polypeptide sequencee3
- Comparator
- untreated cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e3“Comparison of the nucleotide sequences identified nine point differences in the GS gene between the resistant and untreated cells, leading to eight amino acid substitutions in the deduced polypeptide sequence”
Claim · model-written summary
GS mRNA accumulation was higher in resistant cells than in untreated cells.
- Taxon
- soybeanGlycine maxe0
- Material
- resistant cellse4
- Gene
- GSe4
- Outcome
- accumulation of GS gene mRNA transcripte4
- Direction
- highere4
- Comparator
- untreated cellse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e4“Northern hybridization of the GS mRNA showed that the accumulation of GS gene mRNA transcript in resistant cells was higher than that in the untreated cells”
Claim · model-written summary
The exchange of His(249) with tyrosine lowers the sensitivity of the mutated GS enzyme to glufosinate.
- Taxon
- soybeanGlycine maxe0
- Gene
- His(249)e5
- Outcome
- sensitivity of the mutated enzymee5
- Qualifier
- exchange of this residue with tyrosinee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
e5“His(249) is one of the residues implicated in the binding domain for the substrate and inhibitor, and hence the exchange of this residue with tyrosine plays a role in lowering the sensitivity of the mutated enzyme”
Claim · model-written summary
Glufosinate-resistant soybean cells were obtained by direct selection of diploid cells in suspension culture.
- Taxon
- soybeanGlycine maxe0
- Material
- diploid cellse0
- Outcome
- Glufosinate-resistant soybean cellse0
- Setting
- suspension culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glufosinate-resistant soybean cells were achieved through direct selection of diploid cells in the suspension culture”
The same questions in other papers
glufosinate · disease · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.