Abstract
The high affinity potassium transporter, HKT1 from wheat was introduced into Florida wheat in sense and antisense orientation under control of a ubiquitin promoter. Ten transgenic lines expressing the transgene were identified and two of these showed strong down-regulation of the native HKT1 transcript. One line (271) was expressing the antisense construct and the other (223) was expressing a truncated sense construct. The two lines were examined further for phenotype relating to cation transport. Membrane depolarisations were measured in low (0.1 mm) K+ and high (100 mm) NaCl. Under these conditions there was no difference between line 271 and the control at low K+, but at high Na+ there was a rapid depolarisation that was significantly larger in control plants. 22Na uptake was measured in this line and there was a significant decrease in uptake at 100 mm NaCl in the transgenic line when compared with the control. The two transgenic lines were grown at high NaCl (200 mm) and analysed for growth and root sodium content. Lines 271 and 223 showed enhanced growth under salinity when compared with the control and had lower sodium in the root. Secondary ion mass spectrometry (SIMS) analysis of transverse sections of the root showed that Na+ and K+ were strongly localised to stelar regions when compared with other ions, and that the Na+ : K+ ratios were reduced in salt-stressed transgenic tissue when compared with the control.
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Claims 12
Claim · model-written summary
Two transgenic lines strongly down-regulated native HKT1 transcript.
- Taxon
- wheat
Triticum aestivume1 - Material
- two of thesee0
- Gene
- HKT1e0
- Outcome
- native HKT1 transcripte0
- Direction
- down-regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two of these showed strong down-regulation of the native HKT1 transcript”
e1“HKT1 from wheat”
Claim · model-written summary
Line 271 expressed the antisense construct.
- Taxon
- wheatTriticum aestivume1
- Material
- 271e0
- Outcome
- expressing the antisense constructe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One line (271) was expressing the antisense construct”
e1“HKT1 from wheat”
Claim · model-written summary
Line 223 expressed a truncated sense construct.
- Taxon
- wheatTriticum aestivume1
- Material
- 223e0
- Outcome
- expressing a truncated sense constructe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other (223) was expressing a truncated sense construct”
e1“HKT1 from wheat”
Claim · model-written summary
At low potassium, line 271 membrane depolarization did not differ from control.
- Taxon
- wheatTriticum aestivume2
- Material
- line 271e0
- Treatment
- low (0.1 mm) K+e1
- Outcome
- Membrane depolarisationse1
- Direction
- no differencee0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no difference between line 271 and the control at low K+”
e1“Membrane depolarisations were measured in low (0.1 mm) K+ and high (100 mm) NaCl.”
e2“HKT1 from wheat”
Claim · model-written summary
At high sodium, control plants depolarized significantly more than line 271.
- Taxon
- wheatTriticum aestivume2
- Material
- line 271e1
- Treatment
- high Na+e0
- Outcome
- depolarisatione0
- Direction
- largere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at high Na+ there was a rapid depolarisation that was significantly larger in control plants”
e1“line 271 and the control”
e2“HKT1 from wheat”
Claim · model-written summary
Line 271 had significantly lower sodium uptake than control at 100 mm NaCl.
- Taxon
- wheatTriticum aestivume3
- Material
- line 271e2
- Treatment
- 100 mm NaCle0
- Outcome
- 22Na uptakee1
- Direction
- decreasee0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a significant decrease in uptake at 100 mm NaCl in the transgenic line when compared with the control”
e1“22Na uptake was measured in this line”
e2“line 271”
e3“HKT1 from wheat”
Claim · model-written summary
Line 271 showed enhanced growth at high salt relative to control.
- Taxon
- wheatTriticum aestivume2
- Material
- 271e0
- Treatment
- high NaCl (200 mm)e1
- Outcome
- growthe0
- Direction
- enhancede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lines 271 and 223 showed enhanced growth under salinity when compared with the control”
e1“high NaCl (200 mm)”
e2“HKT1 from wheat”
Claim · model-written summary
Line 223 showed enhanced growth at high salt relative to control.
- Taxon
- wheatTriticum aestivume2
- Material
- 223e0
- Treatment
- high NaCl (200 mm)e1
- Outcome
- growthe0
- Direction
- enhancede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lines 271 and 223 showed enhanced growth under salinity when compared with the control”
e1“high NaCl (200 mm)”
e2“HKT1 from wheat”
Claim · model-written summary
Line 271 had lower root sodium under high salt than control.
- Taxon
- wheatTriticum aestivume2
- Material
- 271e0
- Treatment
- high NaCl (200 mm)e1
- Outcome
- sodium in the roote0
- Direction
- lowere0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lines 271 and 223 showed enhanced growth under salinity when compared with the control and had lower sodium in the root.”
e1“high NaCl (200 mm)”
e2“HKT1 from wheat”
Claim · model-written summary
Line 223 had lower root sodium under high salt than control.
- Taxon
- wheatTriticum aestivume2
- Material
- 223e0
- Treatment
- high NaCl (200 mm)e1
- Outcome
- sodium in the roote0
- Direction
- lowere0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lines 271 and 223 showed enhanced growth under salinity when compared with the control and had lower sodium in the root.”
e1“high NaCl (200 mm)”
e2“HKT1 from wheat”
Claim · model-written summary
Sodium and potassium were strongly localized to wheat root stelar regions versus other ions.
- Taxon
- wheatTriticum aestivume2
- Organ / stage
- roote1
- Outcome
- Na+ and K+ were strongly localised to stelar regionse0
- Comparator
- other ionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Na+ and K+ were strongly localised to stelar regions when compared with other ions”
e1“transverse sections of the root”
e2“HKT1 from wheat”
Claim · model-written summary
Salt-stressed transgenic root tissue had reduced Na+:K+ ratio versus control.
- Taxon
- wheatTriticum aestivume2
- Organ / stage
- roote1
- Treatment
- salt-stressed transgenic tissuee0
- Outcome
- Na+ : K+ ratiose0
- Direction
- reducede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Na+ : K+ ratios were reduced in salt-stressed transgenic tissue when compared with the control”
e1“transverse sections of the root”
e2“HKT1 from wheat”
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.