Abstract
HKT1 encodes a high affinity Na+ coupled K+ transporter expressed in the cortical cells of Triticum aestivum roots. To identify regions of the protein involved in the binding and transport of Na+ and K+, mutations were introduced into a domain of HKT1 containing 16 amino acids that are highly conserved across a range of putative K+ transport proteins from different phyla. Two mutations had a significant effect on the functional characteristics of the transporter. A yeast growth assay showed that concentrations of NaCl between 2.5 to 50 mM stimulated the growth of yeast expressing HKT1 containing the E464Q substitution, but not the growth of yeast expressing HKT1. Kinetic analysis confirmed that the E464Q mutation lowered the affinity of HKT1 for Na+ but did not affect its affinity for K+. A second mutation in the same region F463L was created that also lowered the affinity of the transporter for Na+. The importance of these highly conserved amino acid residues is highlighted by the fact that they have remained conserved through evolution. The results of this mutational analysis suggest that this domain in HKT1 plays a role in the binding and transport of Na+.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
NaCl at 2.5 to 50 mM stimulated yeast growth expressing E464Q HKT1.
- Taxon
- Triticum aestivum
Triticum aestivume1 - Material
- E464Qe0
- Treatment
- NaCl between 2.5 to 50 mMe0
- Gene
- HKT1e0
- Outcome
- growth of yeast expressing HKT1 containing the E464Q substitutione0
- Direction
- stimulatede0
- Comparator
- yeast expressing HKT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“concentrations of NaCl between 2.5 to 50 mM stimulated the growth of yeast expressing HKT1 containing the E464Q substitution, but not the growth of yeast expressing HKT1.”
e1“Triticum aestivum roots”
t0“HKT1”
Claim · model-written summary
E464Q lowered HKT1 sodium affinity.
- Taxon
- Triticum aestivumTriticum aestivume1
- Treatment
- E464Q mutatione0
- Gene
- HKT1e0
- Outcome
- affinity of HKT1 for Na+e0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the E464Q mutation lowered the affinity of HKT1 for Na+ but did not affect its affinity for K+.”
e1“Triticum aestivum roots”
t0“HKT1”
Claim · model-written summary
E464Q did not affect HKT1 potassium affinity.
- Taxon
- Triticum aestivumTriticum aestivume1
- Treatment
- E464Q mutatione0
- Gene
- HKT1e0
- Outcome
- affinity for K+e0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the E464Q mutation lowered the affinity of HKT1 for Na+ but did not affect its affinity for K+.”
e1“Triticum aestivum roots”
t0“HKT1”
Claim · model-written summary
F463L lowered HKT1 sodium affinity.
- Taxon
- Triticum aestivumTriticum aestivume1
- Treatment
- F463Le0
- Gene
- HKT1t0
- Outcome
- affinity of the transporter for Na+e0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F463L was created that also lowered the affinity of the transporter for Na+.”
e1“Triticum aestivum roots”
t0“HKT1”
Claim · model-written summary
The conserved HKT1 domain was suggested to mediate sodium binding and transport.
- Taxon
- Triticum aestivumTriticum aestivume1
- Gene
- HKT1e0
- Outcome
- binding and transport of Na+e0
- Qualifier
- this domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this domain in HKT1 plays a role in the binding and transport of Na+.”
e1“Triticum aestivum roots”
t0“HKT1”
The same questions in other papers
sodium chloride · growth · Triticum aestivum (wheat)Results differ
23 papers in the same direction as this paper · 113 in the opposite direction
- positive
- negative
- negative
133 papers more in this group
See all 137 papers →loss of function · physiology: ion balance · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.