Abstract
Xanthomonas oryzae pv. oryzicola (Xoc) causes a destructive bacterial leaf streak disease in rice. Some of the gene products annotated as hypothetical proteins in the genome of Xoc may contribute to its virulence in rice. A mutant, Mxoc1679, screened from our previous Tn5-tagged mutant library for Xoc strain RS105, showed reduced virulence in rice. In this mutant, a gene named as Xoryp_08180 was disrupted by Tn5 insertion. Xoryp_08180 encodes a 1 306-aa hypothetical protein which is highly conserved in Xanthomonas spp. Non-polar mutation of Xoryp_08180 in RS105 strain led to a significant reduction in bacterial virulence and growth in rice, a delayed hypersensitive response (HR) in non-host tobacco, and a decrease in extracellular protease activity. The deficiencies above were restored to wild-type level in the complementary strain by expressing Xoryp_08180 in trans. In addition, the expression of Xoryp_08180 was repressed in hrpG and hrpX mutants in planta but not in a nutrient-rich condition. These results suggested that Xoryp_08180 is a virulence factor required for extracellular protease production, HR induction and full virulence of Xoc.
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Claims 7
Claim · model-written summary
Mxoc1679 showed reduced virulence in rice.
- Taxon
- rice
Oryza sativae0 - Material
- Mxoc1679e0
- Interactor
- Xoce0pathogen
- Outcome
- virulence in ricee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutant, Mxoc1679, screened from our previous Tn5-tagged mutant library for Xoc strain RS105, showed reduced virulence in rice.”
t0“Xanthomonas oryzae pv. oryzicola”
Claim · model-written summary
Xoryp_08180 encodes a conserved 1 306-aa hypothetical protein.
- Taxon
- riceOryza sativat0
- Gene
- Xoryp_08180e0
- Outcome
- 1 306-aa hypothetical proteine0
- Qualifier
- highly conserved in Xanthomonas spp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xoryp_08180 encodes a 1 306-aa hypothetical protein which is highly conserved in Xanthomonas spp.”
t0“Rice”
Claim · model-written summary
Mutation of Xoryp_08180 significantly reduced bacterial virulence and growth in rice.
- Taxon
- riceOryza sativae0
- Treatment
- Non-polar mutation of Xoryp_08180e0
- Interactor
- RS105 straine0pathogen
- Gene
- Xoryp_08180e0
- Outcome
- bacterial virulence and growth in ricee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Non-polar mutation of Xoryp_08180 in RS105 strain led to a significant reduction in bacterial virulence and growth in rice”
Claim · model-written summary
The mutation delayed hypersensitive response in non-host tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Non-polar mutation of Xoryp_08180e1
- Interactor
- Xoce2pathogen
- Outcome
- hypersensitive response (HR)e0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a delayed hypersensitive response (HR) in non-host tobacco”
e1“Non-polar mutation of Xoryp_08180 in RS105 strain”
e2“Xanthomonas oryzae pv. oryzicola (Xoc) causes a destructive bacterial leaf streak disease in rice.”
Claim · model-written summary
The mutation decreased extracellular protease activity.
- Taxon
- riceOryza sativat0
- Treatment
- Non-polar mutation of Xoryp_08180e1
- Interactor
- Xoce2pathogen
- Gene
- Xoryp_08180e1
- Outcome
- extracellular protease activitye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a decrease in extracellular protease activity.”
t0“Rice”
e1“Non-polar mutation of Xoryp_08180 in RS105 strain”
e2“Xanthomonas oryzae pv. oryzicola (Xoc) causes a destructive bacterial leaf streak disease in rice.”
Claim · model-written summary
Complementation restored the reported deficiencies to wild-type level.
- Taxon
- riceOryza sativat0
- Treatment
- expressing Xoryp_08180 in transe0
- Interactor
- Xoce1pathogen
- Gene
- Xoryp_08180e0
- Outcome
- deficiencies abovee0
- Direction
- restorede0
- Comparator
- wild-type levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deficiencies above were restored to wild-type level in the complementary strain by expressing Xoryp_08180 in trans.”
t0“Rice”
e1“Xanthomonas oryzae pv. oryzicola (Xoc) causes a destructive bacterial leaf streak disease in rice.”
Claim · model-written summary
Xoryp_08180 expression was repressed in hrpG and hrpX mutants in planta but not in nutrient-rich conditions.
- Taxon
- riceOryza sativat0
- Material
- hrpG and hrpX mutantse0
- Interactor
- Xoce1pathogen
- Gene
- Xoryp_08180e0
- Outcome
- expression of Xoryp_08180e0
- Direction
- repressede0
- Qualifier
- in planta but not in a nutrient-rich conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of Xoryp_08180 was repressed in hrpG and hrpX mutants in planta but not in a nutrient-rich condition.”
t0“Rice”
e1“Xanthomonas oryzae pv. oryzicola (Xoc) causes a destructive bacterial leaf streak disease in rice.”
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